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- Table of Contents
COL2A1 antibody (PA2141) performs well in WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 31 Publications
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Anti-COL2A1 antibody, PA2141, Western blotting
All lanes: Anti COL2A1 (PA2141) at 0.5ug/ml
Lane 1: COLO320 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MCF-7Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 134 KD
Observed bind size: 170KD
All lanes: Anti COL2A1 (PA2141) at 0.5ug/ml
Lane 1: COLO320 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MCF-7Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 134 KD
Observed bind size: 170KD
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Anti-Collagen II/COL2A1 Antibody Picoband®
COL2A1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | WB |
Tested Applications
COL2A1 antibody was positively detected by WB in:
COLO320 Whole Cell, HELA Whole Cell, MCF-7Whole Cell, A549 Whole Cell, JURKAT Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Collagen II/COL2A1 Antibody Picoband®
View all COL2A1 Antibodies
View all COL2A1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen II, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
170 kDa
Calculated Molecular Weight
141.8 kDa
Background of COL2A1
Collagen, type II, alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocvtes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.
Cite This Product
Anti-Collagen II/COL2A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2141)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rat
95%
High Homology
Rabbit
95%
High Homology
Cow
95%
High Homology
Sheep
95%
High Homology
Dog
95%
High Homology
Mouse
90%
High Homology
Zebrafish
80%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for COL2A1 antibody
Publications
Application:
Effects of Angelicae Pubescentis and Loranthi Decotion on repairing knee joint cartilages in rats
PMID: 29233184
Microfluidics-based optimization of neuroleukin-mediated regulation of articular chondrocyte proliferation
PMID: 26573126
Nano-hydroxyapatite/collagen film as a favorable substrate to maintain the phenotype and promote the growth of chondrocytes cultured in vitro
PMID: 29393382
(+)-Cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells by activating vitamin D receptor
PMID: 29218094
Oestrogen and parathyroid hormone alleviate lumbar intervertebral disc degeneration in ovariectomized rats and enhance Wnt/?-catenin pathway activity
PMID: 27279629
Establishment of a rat model for osteoarthritis resulting from anterior cruciate ligament rupture and its significance
PMID: 26668592
Neuroleukin/Autocrine Motility Factor Receptor Pathway Promotes Proliferation of Articular Chondrocytes through Activation of AKT and Smad2/3
PMID: 26459914
Construction of tissue-engineered laryngeal cartilage with a hollow, semi-flared shape using poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) as a scaffold.
PMID: 25780456
Hypoxia Enhances Protective Effect of Placental-Derived Mesenchymal Stem Cells on Damaged Intestinal Epithelial Cells by Promoting Secretion of Insulin-Like Growth Factor-1
PMID: 24473145
Protective effects of baicalin on rabbit articular chondrocytes in vitro
PMID: 28413465
Use of a biological reactor and platelet-rich plasma for the construction of tissue-engineered bone to repair articular cartilage defects
PMID: 27446265
Oral Resveratrol Prevents Osteoarthritis Progression in C57BL/6J Mice Fed a High-Fat Diet
PMID: 27104565
Effect of anterior cervical discectomy and fusion on adjacent segments in rabbits
PMID: 25550944
Qin X, Jin P, Jiang T, Li M, Tan J, Wu H, Zheng L, Zhao J. Med Sci Monit. 2018 Jan 27;24:539-547. A Human Chondrocyte-Derived In Vitro Model of Alcohol-Induced and Steroid-Induced Femoral Head Necrosis.
PMID: 29374435
Chen H, Yang X, Liao Y, Zeng X, Liang P, Kang N, Tan J, Liang Z. J Biomed Mater Res B Appl Biomater. 2011 Feb;96(2):267-75. Doi: 10.1002/Jbm.B.31762. Epub 2010 Nov 17. Mri And Histologic Analysis Of Collagen Type Ii Sponge On Repairing The Cartila...
PMID: 21210506
Li Jw, Guo Xl, He Cl, Tuo Yh, Wang Z, Wen J, Jin D. Chin Med J (Engl). 2011 Oct;124(19):3080-6. In Vitro Chondrogenesis Of The Goat Bone Marrow Mesenchymal Stem Cells Directed By Chondrocytes In Monolayer And 3-Dimetional Indirect Co-Culture System.
PMID: 22040560
You M, Peng G, Li J, Ma P, Wang Z, Shu W, Peng S, Chen Gq. Biomaterials. 2011 Mar;32(9):2305-13. Doi: 10.1016/J.Biomaterials.2010.12.009. Epub 2010 Dec 28. Chondrogenic Differentiation Of Human Bone Marrow Mesenchymal Stem Cells On Polyhydroxyalka...
PMID: 21190731
Li H, Yang Hs, Wu Tj, Zhang Xy, Jiang Wh, Ma Ql, Chen Yx, Xu Y, Li S, Hua Zc. J Cell Physiol. 2010 Jun;223(3):610-22. Doi: 10.1002/Jcp.22052. Proteomic Analysis Of Early-Response To Mechanical Stress In Neonatal Rat Mandibular Condylar Chondrocytes.
PMID: 20127708
Li J, Wang J, Zou Y, Zhang Y, Long D, Lei L, Tan L, Ye R, Wang X, Zhao Z. Biomaterials. 2012 Nov;33(33):8395-405. Doi: 10.1016/J.Biomaterials.2012.08.019. Epub 2012 Aug 24. The Influence Of Delayed Compressive Stress On Tgf-??1-Induced Chondrogeni...
PMID: 22922021
Ye R, Hao J, Song J, Zhao Z, Fang S, Wang Y, Li J. J Cell Biochem. 2014 Jun;115(6):1089-96. Doi: 10.1002/Jcb.24743. Microenvironment Is Involved In Cellular Response To Hydrostatic Pressures During Chondrogenesis Of Mesenchymal Stem Cells.
PMID: 24357502
Zhang W, Zhang F, Shi H, Tan R, Han S, Ye G, Pan S, Sun F, Liu X. Plos One. 2014 Feb 18;9(2):E88794. Doi: 10.1371/Journal.Pone.0088794. Ecollection 2014. Comparisons Of Rabbit Bone Marrow Mesenchymal Stem Cell Isolation And Culture Methods In Vitro.
PMID: 24558428
Han Zc, Zhang Hn, Wang Yz, Lv Cy, Xu Zy. Saudi Med J. 2014 May;35(5):435-41. Effect Of The Human Insulin-Like Growth Factor 1 Gene Transfection To Human Umbilical Cord Blood Mesenchymal Stem Cells.
PMID: 24825802
Qian J, Liang J, Wang Y, Wang H. Exp Ther Med. 2014 Aug;8(2):377-383. Epub 2014 May 29. Effect Of Passive Motion On Articular Cartilage In Rat Osteoarthritis.
PMID: 25009586
Li X, Du M, Liu X, Wu M, Ye H, Lin J, Chen W, Wu G. Int J Mol Med. 2010 Mar;25(3):393-9. Millimeter Wave Treatment Inhibits No-Induced Apoptosis Of Chondrocytes Through The P38Mapk Pathway.
PMID: 20127044
Lin Q, Fu Q, Zhang Y, Wang H, Liu Z, Zhou J, Duan C, Wang Y, Wu K, Wang C. Eur J Heart Fail. 2010 Nov;12(11):1179-85. Doi: 10.1093/Eurjhf/Hfq144. Epub 2010 Sep 3. Tumourigenesis In The Infarcted Rat Heart Is Eliminated Through Differentiation And ...
PMID: 20817694
Cai B, Zhang Ag, Zhang X, Ge Wj, Dai Gd, Tan Xl, Roodrajeetsing G, Cai Jp. Biomed Res Int. 2015;2015:792161. Doi: 10.1155/2015/792161. Epub 2015 Jun 7. Promoting Effects On Proliferation And Chondrogenic Differentiation Of Bone Marrow-Derived Mese...
PMID: 26137494
Ye J, Wu G, Li X, Li Z, Zheng C, Liu X, Ye H. Evid Based Complement Alternat Med. 2015;2015:464161. Doi: 10.1155/2015/464161. Epub 2015 Jan 29. Millimeter Wave Treatment Inhibits Apoptosis Of Chondrocytes Via Regulation Dynamic Equilibrium Of Intr...
PMID: 25705239
Zhang Lf, Ding Wh, Shi Lb, Li K, Haom Yj, Ke Yn, Tang Zs. Clin Exp Pharmacol Physiol. 2010 Apr;37(4):477-81. Doi: 10.1111/J.1440-1681.2009.05336.X. Epub 2009 Nov 23. Effects Of Exogenous Urotensin Ii On Vascular Remodelling After Balloon Injury.
PMID: 19930425
Fu Z, Liu P, Yang D, Wang F, Yuan L, Lin Z, Jiang J. Int J Mol Med. 2012 Oct;30(4):805-10. Doi: 10.3892/Ijmm.2012.1073. Epub 2012 Jul 20. Interleukin-18-Induced Inflammatory Responses In Synoviocytes And Chondrocytes From Osteoarthriti...
PMID: 22825306
Liu L, Liu Q, Lin X, Wei Qj, Zheng L. In Vitro Cell Dev Biol Anim. 2014 Dec;50(10):982-91. Doi: 10.1007/S11626-014-9795-5. Epub 2014 Aug 15. Effect Of Jeztc, A Synthetic Compound, On Proliferation And Phenotype Maintenance Of Rabbit Articular Chon...
PMID: 25124868
Liu Q, Lu Z, Wu H, Zheng L. Tohoku J Exp Med. 2015;235(3):201-13. Doi: 10.1620/Tjem.235.201. Chondroprotective Effects Of Taurine In Primary Cultures Of Human Articular Chondrocytes.
PMID: 25765089
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Product Promise
We promise Anti-Collagen II/COL2A1 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA2141
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026