Anti-Collagen Type IV COL4A1 Antibody

Product Name

Anti-Collagen Type IV COL4A1 Antibody

View all COL4A1-COL4A6 Antibodies

SKU/Catalog Number

A01411

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Collagen Type IV COL4A1 Antibody (Catalog # A01411). Tested in Dot blot, IHC applications. This antibody reacts with Human, Bovine.

Storage & Handling

Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening. (Ship on wet ice.)

Cite This Product

Anti-Collagen Type IV COL4A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01411)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Collagen Type IV from human and bovine placenta

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01411 is reactive to COL4A1-COL4A6 in Bovine, Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

160.6 kDa

Background of COL4A1-COL4A6

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Boster extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01411 is guaranteed for ELISA, IP, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:50,000
IHC: 1:50 - 1:200
IF Microscopy: User optimized
IP: 1:100
WB: 1:1,000 - 1:10,000
Anti-Collagen Type IV has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.

Validation Images & Assay Conditions

See full target information COL4A1-COL4A6

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