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- Table of Contents
COL6A1 antibody (M02226) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IHC analysis of Collagen Type VI/COL6A1 using anti-Collagen Type VI/COL6A1 antibody (M02226).
Collagen Type VI/COL6A1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-Collagen Type VI/COL6A1 Antibody (M02226) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Collagen Type VI/COL6A1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-Collagen Type VI/COL6A1 Antibody (M02226) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Collagen VI COL6A1 Rabbit Monoclonal Antibody
COL6A1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
COL6A1 antibody was positively detected by WB in:
human PC-3 whole cell, rat ovary tissue, mouse ovary tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
COL6A1 antibody was positively detected by IHC in:
mouse kidney tissue, mouse lung tissue, mouse spleen tissue, mouse liver tissue, rat kidney tissue, rat lung tissue, rat spleen tissue, rat liver tissue, human placenta tissue, human lung cancer tissue, human lymphoma tissue, human ovarian cancer tissue, human breast cancer tissue, human esophageal cancer tissue, human liver cancer tissue, human colon cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-Collagen VI COL6A1 Rabbit Monoclonal Antibody
View all COL6A1 Antibodies
View all COL6A1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HGG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Collagen VI
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
147 kDa
Calculated Molecular Weight
108.5 kDa
Cite This Product
Anti-Collagen VI COL6A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02226)
Protocols
Western Blot protocol for COL6A1 antibody
Immunohistochemistry (IHC) protocol for COL6A1 antibody
Publications
Application:
Species:
Bone marrow mesenchymal stem cells ameliorated kidney fibrosis by attenuating TLR4/NF-κB in diabetic rats
Published: 2020-09-11
Journal: LIFE SCIENCES
Application: IHC
Impact Factor: 3.65
Species: Rat
PMID: 32926926
Review
Have you used Anti-Collagen VI COL6A1 Rabbit Monoclonal Antibody?
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1 Review For Anti-Collagen VI COL6A1 Rabbit Monoclonal Antibody
Western blot analysis was performed using the COL6A1 antibody to detect COL6A1 protein expression in the mouse hippocampus.
| SKU | M02226 |
|---|---|
| Application | Western Blot |
| Sample | Mouse hippocampus tissue |
| Sample Processing Description | The mouse hippocampus was lysed in RIPA buffer supplemented with a protease inhibitor cocktail. After protein quantification, samples were mixed with 5× protein loading buffer and denatured by heating at 100°C for 10 minutes. Five microliters of each protein sample were loaded per lane onto SDS-PAGE. |
| Primary Antibody | Anti-Collagen VI COL6A1 Rabbit Monoclonal Antibody |
| Primary Incubation | overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | 1 hour in room temperature |
| Detection | Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:ChemiDoc MP |
| Results Summary | Western blot analysis was performed using the COL6A1 antibody to detect COL6A1 protein expression in the mouse hippocampus. Although minor non-specific bands were observed, they did not affect the trend analysis, indicating that the antibody is suitable for detecting the target protein in this tissue. |
Changyang Yu, LUTCM
Verified customer
Submitted 2025-11-06
Product Promise
We promise Anti-Collagen VI COL6A1 Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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M02226
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026