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Product Info Summary
| SKU: | A02226 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Bovine, Human |
| Host: | Rabbit |
| Application: | ELISA, IP, IHC, WB |
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Product info
Product Name
Anti-Collagen Type VI COL6A1 Antibody
View all COL6A1-COL6A3 Antibodies
SKU/Catalog Number
A02226
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Collagen Type VI COL6A1 Antibody (Catalog # A02226). Tested in Dot blot, IHC applications. This antibody reacts with Human, Bovine.
Storage & Handling
Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening. (Ship on wet ice.)
Cite This Product
Anti-Collagen Type VI COL6A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02226)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Collagen Type VI from human and bovine placenta
Reactive Species
A02226 is reactive to COL6A1-COL6A3 in Bovine, Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
108.5 kDa
Background of COL6A1-COL6A3
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Boster extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02226 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
IHC: 1:50 - 1:200
IP: 1:100
WB: 1:500 - 1:5,000
Anti-Collagen Type VI has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.
Validation Images & Assay Conditions
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Boster anti collagen VI antibody (600-401-108 Lot 26009, 1:400 45 min RT) showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA
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Dot Blot results using Rabbit Anti-Collagen VI Antibody. Sample: A) Human Collagen VI , B) Bovine/Human Collagen VI . Load: 1) 1000ng, 2) 333.33ng, 3) 111.11ng, 4) 37.03ng, 5) 12.35ng. Primary Antibody: Rabbit Anti-Collagen VI antibody at 5µg/mL at RT for 1hr. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated 1:40,000 at RT for 30mins. Blocking: BlockOut® Universal Blocking Buffer at RT for 30mins.
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Western blot results using Rabbit Anti-Collagen Type VI Antibody. Lane: 1) Human Collagen Type VI reduced and boiled, 3) Human Collagen Type VI reduced and not boiled,5) Opal prestain molecular weight marker , 7) Human Collagen Type VI not reduced and boiled, 9) Human Collagen Type VI not reduced and not boiled. Lanes 2, 4, 6, 8 blank. Load: 10µg. Primary Antibody: Rabbit Anti-Collagen Type VI Antibody 1:500 overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit IgG HRP conjugated 1:40,000 at RT for 30mins. Blocking: BlockOut® Universal Blocking Buffer at RT for 1hr.
Specific Publications For Anti-Collagen Type VI COL6A1 Antibody (A02226)
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