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Product Info Summary
| SKU: | A01696 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-COT MAP3K8 Antibody
SKU/Catalog Number
A01696
Size
100μl
Form
Liquid
Description
Boster Bio Anti-COT MAP3K8 Antibody catalog # A01696. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-COT MAP3K8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01696)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human COT. AA range:256-305
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01696 is reactive to MAP3K8 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
52.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01696 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using COT Polyclonal antibody.
Specific Publications For Anti-COT MAP3K8 Antibody (A01696)
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7 Customer Q&As for Anti-COT MAP3K8 Antibody
Question
Will A01696 anti-COT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
As indicated on the product datasheet, A01696 anti-COT antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-31
Question
My question regarding product A01696, anti-COT antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01696 anti-COT antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-07
Question
Does anti-COT antibody A01696 work on bovine WB with t-cell?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
Our lab technicians have not tested anti-COT antibody A01696 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-COT antibody A01696 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-05
Question
Will anti-COT antibody A01696 work for WB with cervix carcinoma?
V. Wu
Verified customer
Asked: 2019-07-01
Answer
According to the expression profile of cervix carcinoma, MAP3K8 is highly expressed in cervix carcinoma. So, it is likely that anti-COT antibody A01696 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2019-07-01
Question
I was wanting to use your anti-COT antibody for WB for rat cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma identification?
F. Roberts
Verified customer
Asked: 2017-11-20
Answer
As indicated on the product datasheet, A01696 anti-COT antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-20
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-COT antibody A01696. Let me know if you need anything else.
R. Johnson
Verified customer
Asked: 2016-02-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-02-08
Question
Is a blocking peptide available for product anti-COT antibody (A01696)?
M. Wu
Verified customer
Asked: 2015-03-04
Answer
We do provide the blocking peptide for product anti-COT antibody (A01696). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-03-04
