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Product Info Summary
| SKU: | A01696 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-COT MAP3K8 Antibody
See all MAP3K8/Tpl2/COT products
SKU/Catalog Number
A01696
Size
100μl
Form
Liquid
Description
Boster Bio Anti-COT MAP3K8 Antibody catalog # A01696. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-COT MAP3K8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01696)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Isotype
IgG
Immunogen
Synthesized peptide derived from human Cot around the non-phosphorylation site of T290.
Reactive Species
A01696 is reactive to MAP3K8 in Human, Mouse, Rat
Applications
A01696 is guaranteed for WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using COT Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For MAP3K8 (Source: Uniprot.Org, NCBI)
Uniprot ID
P41279
Gene ID
1326
Gene Name
MAP3K8
Full Name
Mitogen-activated protein kinase kinase kinase 8
Weight
52.925kDa
Superfamily
protein kinase superfamily
Alternative Names
Cancer Osaka thyroid oncogene; c-COT; cot (cancer Osaka thyroid) oncogene; COT; COTMEKK8; EC 2.7.11.25; EST; ESTF; ESTFLJ10486; Ewing sarcoma transformant; MAP3K8; mitogen-activated protein kinase kinase kinase 8; Proto-oncogene c-Cot; proto-oncogene serine/threoine protein kinase; Serine/threonine-protein kinase cot; TPL2; Tpl-2; Tumor progression locus 2; tumor progression locus-2 MAP3K8 AURA2, COT, EST, ESTF, MEKK8, TPL2, Tpl-2, c-COT mitogen-activated protein kinase kinase kinase 8 mitogen-activated protein kinase kinase kinase 8|Ewing sarcoma transformant|augmented in rheumatoid arthritis 2|cot (cancer Osaka thyroid) oncogene|proto-oncogene c-Cot|proto-oncogene serine/threoine protein kinase|tumor progression locus 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MAP3K8, check out the MAP3K8 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MAP3K8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-COT MAP3K8 Antibody (A01696)
No publications found for A01696
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
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7 Customer Q&As for Anti-COT MAP3K8 Antibody
Question
Will A01696 anti-COT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
As indicated on the product datasheet, A01696 anti-COT antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-31
Question
My question regarding product A01696, anti-COT antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01696 anti-COT antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-07
Question
Does anti-COT antibody A01696 work on bovine WB with t-cell?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
Our lab technicians have not tested anti-COT antibody A01696 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-COT antibody A01696 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-05
Question
Will anti-COT antibody A01696 work for WB with cervix carcinoma?
V. Wu
Verified customer
Asked: 2019-07-01
Answer
According to the expression profile of cervix carcinoma, MAP3K8 is highly expressed in cervix carcinoma. So, it is likely that anti-COT antibody A01696 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2019-07-01
Question
I was wanting to use your anti-COT antibody for WB for rat cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma identification?
F. Roberts
Verified customer
Asked: 2017-11-20
Answer
As indicated on the product datasheet, A01696 anti-COT antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-20
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-COT antibody A01696. Let me know if you need anything else.
R. Johnson
Verified customer
Asked: 2016-02-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-02-08
Question
Is a blocking peptide available for product anti-COT antibody (A01696)?
M. Wu
Verified customer
Asked: 2015-03-04
Answer
We do provide the blocking peptide for product anti-COT antibody (A01696). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-03-04
