Anti-COT MAP3K8 Antibody

Boster Bio Anti-COT MAP3K8 Antibody catalog # A01696. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A01696
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-COT MAP3K8 Antibody

See all MAP3K8/Tpl2/COT products

SKU/Catalog Number

A01696

Size

100μl

Form

Liquid

Description

Boster Bio Anti-COT MAP3K8 Antibody catalog # A01696. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-COT MAP3K8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01696)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from human Cot around the non-phosphorylation site of T290.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01696 is reactive to MAP3K8 in Human, Mouse, Rat

Applications

A01696 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For MAP3K8 (Source: Uniprot.Org, NCBI)

Uniprot ID

P41279

Gene ID

1326

Gene Name

MAP3K8

Full Name

Mitogen-activated protein kinase kinase kinase 8

Weight

52.925kDa

Superfamily

protein kinase superfamily

Alternative Names

Cancer Osaka thyroid oncogene; c-COT; cot (cancer Osaka thyroid) oncogene; COT; COTMEKK8; EC 2.7.11.25; EST; ESTF; ESTFLJ10486; Ewing sarcoma transformant; MAP3K8; mitogen-activated protein kinase kinase kinase 8; Proto-oncogene c-Cot; proto-oncogene serine/threoine protein kinase; Serine/threonine-protein kinase cot; TPL2; Tpl-2; Tumor progression locus 2; tumor progression locus-2 MAP3K8 AURA2, COT, EST, ESTF, MEKK8, TPL2, Tpl-2, c-COT mitogen-activated protein kinase kinase kinase 8 mitogen-activated protein kinase kinase kinase 8|Ewing sarcoma transformant|augmented in rheumatoid arthritis 2|cot (cancer Osaka thyroid) oncogene|proto-oncogene c-Cot|proto-oncogene serine/threoine protein kinase|tumor progression locus 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MAP3K8, check out the MAP3K8 Infographic

MAP3K8 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MAP3K8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Anti-COT MAP3K8 Antibody

Question

Will A01696 anti-COT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

As indicated on the product datasheet, A01696 anti-COT antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-31

Question

My question regarding product A01696, anti-COT antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01696 anti-COT antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-07

Question

Does anti-COT antibody A01696 work on bovine WB with t-cell?

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

Our lab technicians have not tested anti-COT antibody A01696 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-COT antibody A01696 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine t-cell in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-05

Question

Will anti-COT antibody A01696 work for WB with cervix carcinoma?

V. Wu

Verified customer

Asked: 2019-07-01

Answer

According to the expression profile of cervix carcinoma, MAP3K8 is highly expressed in cervix carcinoma. So, it is likely that anti-COT antibody A01696 will work for WB with cervix carcinoma.

Boster Scientific Support

Answered: 2019-07-01

Question

I was wanting to use your anti-COT antibody for WB for rat cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma identification?

F. Roberts

Verified customer

Asked: 2017-11-20

Answer

As indicated on the product datasheet, A01696 anti-COT antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-11-20

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-COT antibody A01696. Let me know if you need anything else.

R. Johnson

Verified customer

Asked: 2016-02-08

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-02-08

Question

Is a blocking peptide available for product anti-COT antibody (A01696)?

M. Wu

Verified customer

Asked: 2015-03-04

Answer

We do provide the blocking peptide for product anti-COT antibody (A01696). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-03-04

Size

Total: $299

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A01696
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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