Anti-CRISP2 Antibody

Product Name

Anti-CRISP2 Antibody

View all CRISP2 Antibodies

SKU/Catalog Number

A10681

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-CRISP2 Antibody (Catalog # A10681). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

CRISP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-CRISP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10681)

Host

Rabbit

Contents

CRISP2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

CRISP2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human CRISP2. The immunogen is located within the first 50 amino acids of CRISP2.

Reactive Species

A10681 is reactive to CRISP2 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

27.3 kDa

Background of CRISP2

The cysteine-rich secretory proteins (CRISP) family is a group of four proteins that are strongly expressed in the male reproductive tract and have been implicated in having roles in male fertility. CRISP2, also known as TPX1, has been implicated in the adhesion between spermatids and Sertoli cells, and with CRISP1, is thought to be involved in sperm-egg fusion. CRISP2 has been shown to regulate the Ca2+ influx through ryanodine receptors (RYR) and may influence the acrosome reaction or sperm motility. CRISP2 has also been shown to bind to the mitogen-activated protein kinase kinase kinase 11 (MAP3K11) and localizes to the developing acrosome, suggesting this CRISP2-MAP3K11 complex may have a role in acrosome development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10681 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CRISP2 antibody can be used for detection of CRISP2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information CRISP2

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