Product Info Summary
SKU: | M00799 |
---|---|
Size: | 0.1 ml |
Reactive Species: | Human, Mouse |
Host: | Mouse |
Application: | IP, ICC, WB |
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Product info
Product Name
Anti-CSK Purified Monoclonal Antibody
SKU/Catalog Number
M00799
Size
0.1 ml
Form
Liquid
Description
Boster Bio Anti-CSK Purified Monoclonal Antibody (Catalog# M00799). Tested in WB, IP, ICC application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-CSK Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00799)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
CSK-04
Isotype
Mouse IgG1
Immunogen
Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450. The mouse monoclonal antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa intracellular non-receptor protein tyrosine kinase.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00799 is reactive to CSK in Human, Mouse
Applications
M00799 is guaranteed for IP, ICC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
50.704kDa
Background of CSK
C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them it lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases, that are localized in lipid rafts, is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line.
Western blotting: The recommended dilution of the antibody CSK-04 is 1:250-1:300; weak non-specific band of cca 60 kDa is detected in human cell lines in lower antibody dilutions.
Immunoprecipitation: The immunosorbent (CSK-04 antibody covalently bound to beads) immunoprecipitates Csk from cell lysates.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Immunocytochemistry staining of CSK using Anti-CSK Purified Monoclonal Antibody (M00799).
Immunocytochemistry staining of Csk in human HeLa cell line using anti-Csk (CSK-04; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).Protein Target Info & Infographic
Gene/Protein Information For CSK (Source: Uniprot.org, NCBI)
Gene Name
CSK
Full Name
Tyrosine-protein kinase CSK
Weight
50.704kDa
Superfamily
protein kinase superfamily
Alternative Names
C-Src kinase; c-src tyrosine kinase; EC 2.7.10; EC 2.7.10.2; MGC117393; Protein-tyrosine kinase CYL; tyrosine-protein kinase CSK CSK C-terminal Src kinase tyrosine-protein kinase CSK|C-Src kinase|CSK, non-receptor tyrosine kinase|c-src tyrosine kinase|protein-tyrosine kinase CYL
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CSK, check out the CSK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CSK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CSK Purified Monoclonal Antibody (M00799)
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No publications found for M00799
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Recommended Resources
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