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Product Info Summary
| SKU: | M00799 |
|---|---|
| Size: | 0.1 ml |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | IP, ICC, WB |
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Product info
Product Name
Anti-CSK Purified Monoclonal Antibody
SKU/Catalog Number
M00799
Size
0.1 ml
Form
Liquid
Description
Boster Bio Anti-CSK Purified Monoclonal Antibody (Catalog# M00799). Tested in WB, IP, ICC application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-CSK Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00799)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
CSK-04
Isotype
Mouse IgG1
Immunogen
Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450. The mouse monoclonal antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa intracellular non-receptor protein tyrosine kinase.
Reactive Species
M00799 is reactive to CSK in Human, Mouse
Observed Molecular Weight
42 kDa
Calculated molecular weight
50.7 kDa
Background of CSK
C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them it lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases, that are localized in lipid rafts, is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00799 is guaranteed for IP, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line.
Western blotting: The recommended dilution of the antibody CSK-04 is 1:250-1:300; weak non-specific band of cca 60 kDa is detected in human cell lines in lower antibody dilutions.
Immunoprecipitation: The immunosorbent (CSK-04 antibody covalently bound to beads) immunoprecipitates Csk from cell lysates.
Validation Images & Assay Conditions
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Immunocytochemistry staining of Csk in human HeLa cell line using anti-Csk (CSK-04; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Specific Publications For Anti-CSK Purified Monoclonal Antibody (M00799)
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