Anti-CSK Purified Monoclonal Antibody

CSK antibody

Boster Bio Anti-CSK Purified Monoclonal Antibody (Catalog# M00799). Tested in WB, IP, ICC application(s). This antibody reacts with Human, Mouse.

Product Info Summary

SKU: M00799
Size: 0.1 ml
Reactive Species: Human, Mouse
Host: Mouse
Application: IP, ICC, WB

Product Name

Anti-CSK Purified Monoclonal Antibody

View all CSK Antibodies

SKU/Catalog Number

M00799

Size

0.1 ml

Form

Liquid

Description

Boster Bio Anti-CSK Purified Monoclonal Antibody (Catalog# M00799). Tested in WB, IP, ICC application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-CSK Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00799)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

CSK-04

Isotype

Mouse IgG1

Immunogen

Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450. The mouse monoclonal antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa intracellular non-receptor protein tyrosine kinase.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00799 is reactive to CSK in Human, Mouse

Applications

M00799 is guaranteed for IP, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

50.704kDa

Background of CSK

C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them it lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases, that are localized in lipid rafts, is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line.
Western blotting: The recommended dilution of the antibody CSK-04 is 1:250-1:300; weak non-specific band of cca 60 kDa is detected in human cell lines in lower antibody dilutions.
Immunoprecipitation: The immunosorbent (CSK-04 antibody covalently bound to beads) immunoprecipitates Csk from cell lysates.

Validation Images & Assay Conditions

Gene/Protein Information For CSK (Source: Uniprot.org, NCBI)

Gene Name

CSK

Full Name

Tyrosine-protein kinase CSK

Weight

50.704kDa

Superfamily

protein kinase superfamily

Alternative Names

C-Src kinase; c-src tyrosine kinase; EC 2.7.10; EC 2.7.10.2; MGC117393; Protein-tyrosine kinase CYL; tyrosine-protein kinase CSK CSK C-terminal Src kinase tyrosine-protein kinase CSK|C-Src kinase|CSK, non-receptor tyrosine kinase|c-src tyrosine kinase|protein-tyrosine kinase CYL

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CSK, check out the CSK Infographic

CSK infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CSK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M00799

0.1 ml

$326

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