Product Info Summary
SKU: | M00150-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SHP2/PTPN11 Antibody Picoband™ (monoclonal, 2E6)
View all SHP-2/PTPN11 Antibodies
SKU/Catalog Number
M00150-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SHP2/PTPN11 Antibody Picoband™ (monoclonal, 2E6) catalog # M00150-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SHP2/PTPN11 Antibody Picoband™ (monoclonal, 2E6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00150-2)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
Clone: 2E6
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SHP2, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00150-2 is reactive to PTPN11 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
46659 MW
Background of SHP-2/PTPN11
PTPN11 (Tyrosine-protein phosphatase non-receptor type 11), also known as protein-tyrosine phosphatase 1D (PTP-1D), protein-tyrosine phosphatase 2C (PTP-2C), TYROSINE PHOSPHATASE SHP2 (SHP2), BPTP3, SH-PTP2, SHP-2, SH-PTP3, is an enzyme that in humans is encoded by the PTPN11 gene. PTPN11 is a member of the protein tyrosine phosphatase (PTP) family. The open reading frame consists of 1,779 nucleotides potentially encoding a protein of 593 amino acids with a predicted molecular mass of 68 kD. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00150-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
Validation Images & Assay Conditions
![m00150 2 ptpn11 primary antibodies wb testing 1 m00150 2 ptpn11 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/m/0/m00150-2-ptpn11-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (M00150-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human CCRF-CEM whole cell lysates.
Lane 4: human K562 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: human CACO-2 whole cell lysates,
Lane 7: human Hela whole cell lysates,
Lane 8: rat brain tissue lysates,
Lane 9: rat C6 whole cell lysates,
Lane 10: mouse brain tissue lysates,
Lane 11: mouse Neuro-2a whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SHP2/PTPN11 antigen affinity purified monoclonal antibody (Catalog # M00150-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SHP2/PTPN11 at approximately 70KD. The expected band size for SHP2/PTPN11 is at 70KD.
![m00150 2 ptpn11 primary antibodies ihc testing 2 m00150 2 ptpn11 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/m/0/m00150-2-ptpn11-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of SHP2/PTPN11 using anti SHP2/PTPN11 antibody (M00150-2).
SHP2/PTPN11 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SHP2/PTPN11 Antibody (M00150-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
![m00150 2 ptpn11 primary antibodies ihc testing 3 m00150 2 ptpn11 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/m/0/m00150-2-ptpn11-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of SHP2/PTPN11 using anti SHP2/PTPN11 antibody (M00150-2).
SHP2/PTPN11 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SHP2/PTPN11 Antibody (M00150-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
![m00150 2 ptpn11 primary antibodies ihc testing 4 m00150 2 ptpn11 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/m/0/m00150-2-ptpn11-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of SHP2/PTPN11 using anti SHP2/PTPN11 antibody (M00150-2).
SHP2/PTPN11 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SHP2/PTPN11 Antibody (M00150-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
![m00150 2 ptpn11 primary antibodies ihc testing 5 m00150 2 ptpn11 primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/m/0/m00150-2-ptpn11-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of SHP2/PTPN11 using anti SHP2/PTPN11 antibody (M00150-2).
SHP2/PTPN11 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-SHP2/PTPN11 Antibody (M00150-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
![m00150 2 ptpn11 primary antibodies if testing 6 m00150 2 ptpn11 primary antibodies if testing 6](https://www.bosterbio.com/media/catalog/product/m/0/m00150-2-ptpn11-primary-antibodies-if-testing-6.jpg)
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Figure 6. IF analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (M00150-2).
SHP2/PTPN11 was detected in immunocytochemical section of U251 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-SHP2/PTPN11 Antibody (M00150-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![m00150 2 ptpn11 primary antibodies fcm testing 7 m00150 2 ptpn11 primary antibodies fcm testing 7](https://www.bosterbio.com/media/catalog/product/m/0/m00150-2-ptpn11-primary-antibodies-fcm-testing-7.png)
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Figure 7. Flow Cytometry analysis of A549 cells using anti-SHP2/PTPN11 antibody (M00150-2).
Overlay histogram showing A549 cells stained with M00150-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-SHP2/PTPN11 Antibody (M00150-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PTPN11 (Source: Uniprot.org, NCBI)
Gene Name
PTPN11
Full Name
Tyrosine-protein phosphatase non-receptor type 11
Weight
46659 MW
Superfamily
protein-tyrosine phosphatase family
Alternative Names
BPTP3; EC 3.1.3.48; MGC14433; protein tyrosine phosphatase, non-receptor type 11; protein tyrosine phosphatase-2; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; PTP1D; PTP-1D; PTP2C; PTP-2C; PTP2CNS1; PTPN11; SHP2; SHP-2; SHP2CFC; SHPTP2; SH-PTP2; SH-PTP2Noonan syndrome 1; SH-PTP3; tyrosine-protein phosphatase non-receptor type 11 PTPN11 BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2 protein tyrosine phosphatase non-receptor type 11 tyrosine-protein phosphatase non-receptor type 11|SH2 domain-containing protein tyrosine phosphatase 2|protein-tyrosine phosphatase 1D|protein-tyrosine phosphatase 2C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTPN11, check out the PTPN11 Infographic
![PTPN11 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTPN11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SHP2/PTPN11 Antibody Picoband™ (monoclonal, 2E6) (M00150-2)
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