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Product Info Summary
| SKU: | M03487 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-CYB5R3 Mouse Monoclonal Antibody [Clone ID: OTI1E8]
SKU/Catalog Number
M03487
Size
100 µl
Description
Boster Bio CYB5R3 mouse monoclonal antibody, clone OTI1E8 (formerly 1E8). Catalog# M03487. Tested in IF, IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-CYB5R3 Mouse Monoclonal Antibody [Clone ID: OTI1E8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03487)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1E8
Isotype
IgG1
Immunogen
Full length human recombinant protein of human CYV5R3 (NP_000389) produced in HEK293T cell.
Reactive Species
M03487 is reactive to CYB5R3 in Human
Observed Molecular Weight
34 kDa
Calculated molecular weight
34.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03487 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500~2000
IHC: 1:150
IF: 1:100
Positive Control
WB: human HepG2 whole cell, human Hela whole cell, human 293T whole cell
Validation Images & Assay Conditions
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Western blot analysis of CYB5R3 using anti-CYB5R3 antibody (M03487).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CYB5R3 antigen affinity purified monoclonal antibody (Catalog # M03487) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CYB5R3 at approximately 34 kDa. The expected band size for CYB5R3 is at 34 kDa.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CYB5R3 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CYB5R3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Anti-CYB5R3 mouse monoclonal antibody (M03487) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYB5R3.
Specific Publications For Anti-CYB5R3 Mouse Monoclonal Antibody [Clone ID: OTI1E8] (M03487)
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