Anti-Cyclin D1/CCND1 Antibody

Boster Bio Anti-Cyclin D1/CCND1 Antibody catalog # PA1245-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 42 publication(s).

Product Info Summary

SKU: PA1245-1
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Cyclin D1/CCND1 Antibody

See all Ccnd1 products

SKU/Catalog Number

PA1245-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cyclin D1/CCND1 Antibody catalog # PA1245-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cyclin D1/CCND1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1245-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Cyclin D1 (219-238aa SPNNFLSCYRTTHFLSRVIK), identical to the related rat sequence, and different from the related human sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1245-1 is reactive to Ccnd1 in Human, Mouse, Rat

Applications

PA1245-1 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For Ccnd1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P25322

Gene ID

12443

Gene Name

Ccnd1

Full Name

G1/S-specific cyclin-D1

Weight

33.429kDa

Superfamily

cyclin family

Alternative Names

B-cell lymphoma 1 protein; BCL-1 oncogene; Bcl-1; BCL1D11S287E; CCND1; cyclin D1 (PRAD1: parathyroid adenomatosis 1); Cyclin D1; Cyl-1; G1/S-specific cyclin D1; G1/S-specific cyclin-D1; PRAD1 oncogene; PRAD1; PRAD1B-cell CLL/lymphoma 1; U21B31 Ccnd1|AI327039, CycD1, Cyl-, Cyl-1, PR, PRAD1, bcl-, bcl-1, cD1|cyclin D1|G1/S-specific cyclin-D1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ccnd1, check out the Ccnd1 Infographic

Ccnd1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ccnd1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1245-1 has been cited in 42 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Liu S,Sun Y,Zhao R,Wang Y,Zhang W,Pang W. Isoleucine increases muscle mass through promoting myogenesis and intramyocellular fat deposition. Food Funct.2021 Jan 7;12(1):144-153.doi: 10.1039/d0fo02156c.Epub 2020 Dec 8. PMID:33289736.
Species: Mouse
PA1245-1 usage in article: APP:WB, SAMPLE:MUSCLE TISSUE AND C2C12 CELLS, DILUTION:1:500

Nan W,Li G,Si H,Lou Y,Wang D,Guo R,Zhang H.All-trans-retinoic acid inhibits mink hair follicle growth via inhibiting proliferation and inducing apoptosis of dermal papilla cells through TGF-β2/Smad2/3 pathway. Acta Histochem. 2020 Oct;122(7):151603.doi: 1
Species: American mink
PA1245-1 usage in article: APP:WB, SAMPLE:HAIR FOLLICLES AND DPCS, DILUTION:1:300

Wang S,Zhang C,Zhang X.Downregulation of long non‑coding RNA ANRIL promotes proliferation and migration in hypoxic human pulmonary artery smooth muscle cells.Mol Med Rep.2020 Feb;21(2):589-596.doi:10.3892/mmr.2019.10887.Epub 2019 Dec 17.PMID:31974617; PMC
Species: Human
PA1245-1 usage in article: APP:WB, SAMPLE:HPASMCS, DILUTION:1:200

Wang C,Shao S,Deng L,Wang S,Zhang Y. LncRNA SNHG12 regulates the radiosensitivity of cervical cancer through the miR-148a/CDK1 pathway. Cancer Cell Int.2020 Dec 1;20(1):554.doi:10.1186/s12935-020-01654-5.PMID:33292254;PMCID:PMC7708190.
Species: Human
PA1245-1 usage in article: APP:WB, SAMPLE:SIHA AND HELA CELLS, DILUTION:1:2000

Effects of Buddleja officinalis granules on apoptosis factors Bax, Caspase-3, Fas, and FasL in lacrimal gland cells of castrated male rabbits Genyan Qin, et al. J Ophthalmol. 2019 Feb 5;2019:5916243. doi: 10.1155/2019/5916243. eCollection 2019.

Effects of xanthohumol-rich hop extract on the differentiation of preadipocytes

Overexpression of MYCT1 inhibits proliferation and induces apoptosis in human acute myeloid leukemia HL-60 and KG-1a cells in vitro and in vivo

Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3-E1 cells

LRIG2 promotes the proliferation and cell cycle progression of glioblastoma cells in vitro and in vivo through enhancing PDGFR? signaling

Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian Granular Cells of Mice

Effects of low-intensity ultrasound combined with low-dose carboplatin in an orthotopic hamster model of tongue cancer: A preclinical study

Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis

Fucoidan inhibits lymphangiogenesis by downregulating the expression of VEGFR3 and PROX1 in human lymphatic endothelial cells

Sirt1 AS lncRNA interacts with its mRNA to inhibit muscle formation by attenuating function of miR-34a

The expression of AEG-1 and Cyclin D1 in human bladder urothelial carcinoma and their clinicopathological significance

Overexpression of miR-30a in lung adenocarcinoma A549 cell line inhibits migration and invasion via targeting EYA2

CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment

Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats

Silencing of carboxypeptidase E inhibits cell proliferation, tumorigenicity, and metastasis of osteosarcoma cells

He H, Chen K, Wang F, Zhao L, Wan X, Wang L, Mo Z. Int J Mol Med. 2015 Jun;35(6):1587-95. Doi: 10.3892/Ijmm.2015.2160. Epub 2015 Mar 31. Mir-204-5P Promotes The Adipogenic Differentiation Of Human Adipose-Derived Mesenchymal Stem Cells By Modulati...

Li L, Zhao F, Lu J, Li T, Yang H, Wu C, Liu Y. Plos One. 2014 Apr 23;9(4):E95912. Doi: 10.1371/Journal.Pone.0095912. Ecollection 2014. Notch-1 Signaling Promotes The Malignant Features Of Human Breast Cancer Through Nf-??b Activation.

Liu C, Su T, Li F, Li L, Qin X, Pan W, Feng F, Chen F, Liao D, Chen L. Acta Biochim Biophys Sin (Shanghai). 2010 Jun 15;42(6):396-402. Pi3K/Akt Signaling Transduction Pathway Is Involved In Rat Vascular Smooth Muscle Cell Proliferation Induced By ...

Feng Z, Chen J, Wei H, Gao P, Shi J, Zhang J, Zhao F. Exp Mol Pathol. 2011 Oct;91(2):569-77. Doi: 10.1016/J.Yexmp.2011.06.004. Epub 2011 Jul 12. The Risk Factor Of Gallbladder Cancer: Hyperplasia Of Mucous Epithelium Caused By Gallstones Associate...

Li B, Gao Mh, Chu Xm, Teng L, Lv Cy, Yang P, Yin Qf. Eur J Pharmacol. 2015 Feb 15;749:107-14. Doi: 10.1016/J.Ejphar.2015.01.009. Epub 2015 Jan 21. The Synergistic Antitumor Effects Of All-Trans Retinoic Acid And C-Phycocyanin On The Lung Cancer A5...

Nie D, Huang K, Yin S, Li Y, Xie S, Ma L, Wang X, Wu Y, Xiao J. Leuk Lymphoma. 2012 Dec;53(12):2487-95. Doi: 10.3109/10428194.2012.698273. Epub 2012 Jun 18. Synergistic/Additive Interaction Of Valproic Acid With Bortezomib On Proliferation And Apo...

Wu Y, Tao Y, Chen Y, Xu W. Plos One. 2012;7(11):E48917. Doi: 10.1371/Journal.Pone.0048917. Epub 2012 Nov 7. Rhoc Regulates The Proliferation Of Gastric Cancer Cells Through Interaction With Iqgap1.

Cai H, Wang Z, Zhang Hq, Wang Fr, Yu Cx, Zhang Fx, Gao L, Zhang J, Zhao Jj. Acta Pharmacol Sin. 2014 Jan;35(1):65-73. Doi: 10.1038/Aps.2013.133. Epub 2013 Nov 18. Diosgenin Relieves Goiter Via The Inhibition Of Thyrocyte Proliferation In A Mouse M...

Yang L, Su T, Lv D, Xie F, Liu W, Cao J, Sheikh Ia, Qin X, Li L, Chen L. Acta Biochim Biophys Sin (Shanghai). 2014 Feb;46(2):100-11. Doi: 10.1093/Abbs/Gmt140. Epub 2013 Dec 29. Erk1/2 Mediates Lung Adenocarcinoma Cell Proliferation And Autophagy I...

Du Mj, Chen Xd, Zhou Xl, Wan Yj, Lan B, Zhang Cz, Cao Y. Plos One. 2014 Jun 6;9(6):E99052. Doi: 10.1371/Journal.Pone.0099052. Ecollection 2014. Estrogen Induces Vav1 Expression In Human Breast Cancer Cells.

Huang B, Lu Ys, Li X, Zhu Zc, Li K, Liu Jw, Zheng J, Hu Zl. Int J Clin Exp Pathol. 2014 Apr 15;7(5):2179-84. Ecollection 2014. Androglobin Knockdown Inhibits Growth Of Glioma Cell Lines.

Liu H, Yan Zq, Li B, Yin Sy, Sun Q, Kou Jj, Ye D, Ferns K, Liu Hy, Liu Sl. J Ovarian Res. 2014 Sep 5;7:87. Doi: 10.1186/S13048-014-0087-1. Reduced Expression Of Sox7 In Ovarian Cancer: A Novel Tumor Suppressor Through The Wnt/??-Catenin Signaling ...

Liu J, Ma J, Wu Z, Li W, Zhang D, Han L, Wang F, Reindl Km, Wu E, Ma Q. Bmc Cancer. 2014 Sep 20;14:686. Doi: 10.1186/1471-2407-14-686. Arginine Deiminase Augments The Chemosensitivity Of Argininosuccinate Synthetase-Deficient Pancreatic Cancer Cel...

Zhu Z, Li K, Xu D, Liu Y, Tang H, Xie Q, Xie L, Liu J, Wang H, Gong Y, Hu Z, Zheng J. J Neurooncol. 2013 Mar;112(1):17-25. Doi: 10.1007/S11060-012-1032-Z. Epub 2013 Jan 16. Zfx Regulates Glioma Cell Proliferation And Survival In Vitro And In Vivo.

Liu Y, Zhu Z, Xiong Z, Zheng J, Hu Z, Qiu J. Int J Clin Exp Pathol. 2014 Aug 15;7(9):5750-61. Ecollection 2014. Knockdown Of Protein Tyrosine Phosphatase Receptor U Inhibits Growth And Motility Of Gastric Cancer Cells.

Zhu Z, Liu Y, Li K, Liu J, Wang H, Sun B, Xiong Z, Jiang H, Zheng J, Hu Z. Carcinogenesis. 2014 Aug;35(8):1901-10. Doi: 10.1093/Carcin/Bgu123. Epub 2014 May 29. Protein Tyrosine Phosphatase Receptor U (Ptpru) Is Required For Glioma Growth And Moti...

Deng L, Lu Y, Zhao X, Sun Y, Shi Y, Fan H, Liu C, Zhou J, Nie Y, Wu K, Fan D, Guo X. Biochem Biophys Res Commun. 2013 Oct 18;440(2):322-9. Doi: 10.1016/J.Bbrc.2013.09.079. Epub 2013 Sep 25. Ran Gtpase Protein Promotes Human Pancreatic Cancer Proli...

Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H, Liu M. Plos One. 2013 Oct 23;8(10):E77829. Doi: 10.1371/Journal.Pone.0077829. Ecollection 2013. Down-Regulation Of Mir-129-5P Inhibits Growth And Induces Apoptosis In Laryngeal Squa...

Zhang H, Zhao B, Huang C, Meng Xm, Bian Eb, Li J. Plos One. 2014 May 2;9(5):E95520. Doi: 10.1371/Journal.Pone.0095520. Ecollection 2014. Melittin Restores Pten Expression By Down-Regulating Hdac2 In Human Hepatocelluar Carcinoma Hepg2 Cells.

Han W, Liu Gn. Life Sci. 2010 Feb 13;86(7-8):234-43. Doi: 10.1016/J.Lfs.2009.12.005. Epub 2009 Dec 16. Egr-1 Decoy Odns Inhibit Vascular Smooth Muscle Cell Proliferation And Neointimal Hyperplasia Of Balloon-Injured Arteries In Rat.

Ren J, Huang Q, Xu Y, Yang M, Yang J, Hu K. Anticancer Drugs. 2015 Jul;26(6):599-611. Doi: 10.1097/Cad.0000000000000224. Isoflavone Lupiwighteone Induces Cytotoxic, Apoptotic, And Antiangiogenic Activities In Du-145 Prostate Cancer Cells.

Li K, Zhu Zc, Liu Yj, Liu Jw, Wang Ht, Xiong Zq, Shen X, Hu Zl, Zheng J. Int J Clin Exp Pathol. 2013 Sep 15;6(11):2460-7. Ecollection 2013. Zfx Knockdown Inhibits Growth And Migration Of Non-Small Cell Lung Carcinoma Cell Line H1299.

Li B, Xu Yj, Chu Xm, Gao Mh, Wang Xh, Nie Sm, Yang F, Lv Cy. Immunol Lett. 2013 Nov-Dec;156(1-2):68-81. Doi: 10.1016/J.Imlet.2013.09.025. Epub 2013 Sep 29. Molecular Mechanism Of Inhibitory Effects Of Cd59 Gene On Atherosclerosis In Apoe (-/-) Mice.

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6 Customer Q&As for Anti-Cyclin D1/CCND1 Antibody

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Cyclin D1/CCND1 antibody PA1245-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-24

Question

I see that the anti-Cyclin D1/CCND1 antibody PA1245-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-19

Question

I am interested in to test anti-Cyclin D1/CCND1 antibody PA1245-1 on rat liver for research purposes, then I may be interested in using anti-Cyclin D1/CCND1 antibody PA1245-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

The products we sell, including anti-Cyclin D1/CCND1 antibody PA1245-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-26

Question

Would anti-Cyclin D1/CCND1 antibody PA1245-1 work on bovine WB with placenta?

Verified Customer

Verified customer

Asked: 2019-03-07

Answer

Our lab technicians have not tested anti-Cyclin D1/CCND1 antibody PA1245-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Cyclin D1/CCND1 antibody PA1245-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine placenta in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-03-07

Question

Is this PA1245-1 anti-Cyclin D1/CCND1 antibody reactive to the isotypes of CCND1?

C. Kulkarni

Verified customer

Asked: 2017-11-10

Answer

The immunogen of PA1245-1 anti-Cyclin D1/CCND1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse Cyclin D1(219-238aa SPNNFLSCYRTTHFLSRVIK), identical to the related rat sequence, and different from the related human sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-11-10

Question

I was wanting to use your anti-Cyclin D1/CCND1 antibody for WB for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?

O. Moore

Verified customer

Asked: 2015-06-25

Answer

You can see on the product datasheet, PA1245-1 anti-Cyclin D1/CCND1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-06-25

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