Anti-Cyclin D1/CCND1 Antibody

Ccnd1 antibody

Boster Bio Anti-Cyclin D1/CCND1 Antibody catalog # PA1245-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PA1245-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Cyclin D1/CCND1 Antibody

View all Ccnd1 Antibodies

SKU/Catalog Number

PA1245-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cyclin D1/CCND1 Antibody catalog # PA1245-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cyclin D1/CCND1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1245-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Cyclin D1 (219-238aa SPNNFLSCYRTTHFLSRVIK), identical to the related rat sequence, and different from the related human sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1245-1 is reactive to Ccnd1 in Human, Mouse, Rat

Applications

PA1245-1 is guaranteed for WB Boster Guarantee

Background of Ccnd1

Cyclin D1, also known as CCND1, is a human gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1-S phase of the cell cycle. Amplification or overexpression of cyclin D1 plays pivotal roles in the development of a subset of human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer. The cyclin D1 gene is overexpressed in human breast cancers and is required for oncogene-induced tumorigenesis. Brisken et al. (2003) found that prolactin (PRL; 176760) induced IGF2 (147470) mRNA and IGF2 induced cyclin D1 protein expression in mouse mammary epithelial cultures. And they also concluded that IGF2 is a mediator of prolactin-induced alveologenesis and that prolactin, IGF2, and cyclin D1 are components of a developmental pathway in mammary gland.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For Ccnd1 (Source: Uniprot.org, NCBI)

Uniprot ID

P25322

Gene ID

12443

Gene Name

Ccnd1

Full Name

G1/S-specific cyclin-D1

Weight

33.429kDa

Superfamily

cyclin family

Alternative Names

B-cell lymphoma 1 protein; BCL-1 oncogene; Bcl-1; BCL1D11S287E; CCND1; cyclin D1 (PRAD1: parathyroid adenomatosis 1); Cyclin D1; Cyl-1; G1/S-specific cyclin D1; G1/S-specific cyclin-D1; PRAD1 oncogene; PRAD1; PRAD1B-cell CLL/lymphoma 1; U21B31 Ccnd1|AI327039, CycD1, Cyl-, Cyl-1, PR, PRAD1, bcl-, bcl-1, cD1|cyclin D1|G1/S-specific cyclin-D1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Ccnd1, check out the Ccnd1 Infographic

Ccnd1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Ccnd1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

PA1245-1 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Liu S,Sun Y,Zhao R,Wang Y,Zhang W,Pang W. Isoleucine increases muscle mass through promoting myogenesis and intramyocellular fat deposition. Food Funct.2021 Jan 7;12(1):144-153.doi: 10.1039/d0fo02156c.Epub 2020 Dec 8. PMID:33289736.
Species: Mouse
PA1245-1 usage in article: APP:WB, SAMPLE:MUSCLE TISSUE AND C2C12 CELLS, DILUTION:1:500

Nan W,Li G,Si H,Lou Y,Wang D,Guo R,Zhang H.All-trans-retinoic acid inhibits mink hair follicle growth via inhibiting proliferation and inducing apoptosis of dermal papilla cells through TGF-β2/Smad2/3 pathway. Acta Histochem. 2020 Oct;122(7):151603.doi: 1
Species: American mink
PA1245-1 usage in article: APP:WB, SAMPLE:HAIR FOLLICLES AND DPCS, DILUTION:1:300

Wang S,Zhang C,Zhang X.Downregulation of long non‑coding RNA ANRIL promotes proliferation and migration in hypoxic human pulmonary artery smooth muscle cells.Mol Med Rep.2020 Feb;21(2):589-596.doi:10.3892/mmr.2019.10887.Epub 2019 Dec 17.PMID:31974617; PMC
Species: Human
PA1245-1 usage in article: APP:WB, SAMPLE:HPASMCS, DILUTION:1:200

Wang C,Shao S,Deng L,Wang S,Zhang Y. LncRNA SNHG12 regulates the radiosensitivity of cervical cancer through the miR-148a/CDK1 pathway. Cancer Cell Int.2020 Dec 1;20(1):554.doi:10.1186/s12935-020-01654-5.PMID:33292254;PMCID:PMC7708190.
Species: Human
PA1245-1 usage in article: APP:WB, SAMPLE:SIHA AND HELA CELLS, DILUTION:1:2000

Effects of Buddleja officinalis granules on apoptosis factors Bax, Caspase-3, Fas, and FasL in lacrimal gland cells of castrated male rabbits Genyan Qin, et al. J Ophthalmol. 2019 Feb 5;2019:5916243. doi: 10.1155/2019/5916243. eCollection 2019.

Effects of xanthohumol-rich hop extract on the differentiation of preadipocytes

Overexpression of MYCT1 inhibits proliferation and induces apoptosis in human acute myeloid leukemia HL-60 and KG-1a cells in vitro and in vivo

Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3-E1 cells

LRIG2 promotes the proliferation and cell cycle progression of glioblastoma cells in vitro and in vivo through enhancing PDGFR? signaling

Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian Granular Cells of Mice

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6 Customer Q&As for Anti-Cyclin D1/CCND1 Antibody

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Cyclin D1/CCND1 antibody PA1245-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-24

Question

I see that the anti-Cyclin D1/CCND1 antibody PA1245-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-19

Question

I am interested in to test anti-Cyclin D1/CCND1 antibody PA1245-1 on rat liver for research purposes, then I may be interested in using anti-Cyclin D1/CCND1 antibody PA1245-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

The products we sell, including anti-Cyclin D1/CCND1 antibody PA1245-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-26

Question

Would anti-Cyclin D1/CCND1 antibody PA1245-1 work on bovine WB with placenta?

Verified Customer

Verified customer

Asked: 2019-03-07

Answer

Our lab technicians have not tested anti-Cyclin D1/CCND1 antibody PA1245-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Cyclin D1/CCND1 antibody PA1245-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine placenta in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-03-07

Question

Is this PA1245-1 anti-Cyclin D1/CCND1 antibody reactive to the isotypes of CCND1?

C. Kulkarni

Verified customer

Asked: 2017-11-10

Answer

The immunogen of PA1245-1 anti-Cyclin D1/CCND1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse Cyclin D1(219-238aa SPNNFLSCYRTTHFLSRVIK), identical to the related rat sequence, and different from the related human sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-11-10

Question

I was wanting to use your anti-Cyclin D1/CCND1 antibody for WB for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?

O. Moore

Verified customer

Asked: 2015-06-25

Answer

You can see on the product datasheet, PA1245-1 anti-Cyclin D1/CCND1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-06-25

Size

Conjugation

Total: $315

SKU:PA1245-1

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PA1245-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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