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- Table of Contents
42 Citations 6 Q&As
45 Citations
27 Citations 1 Q&As
21 Citations
1 Citations
2 Citations
Facts about G1/S-specific cyclin-D1.
Cyclin D-CDK4 complexes are important integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity.
Human | |
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Gene Name: | CCND1 |
Uniprot: | P24385 |
Entrez: | 595 |
Belongs to: |
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cyclin family |
B-cell lymphoma 1 protein; BCL-1 oncogene; Bcl-1; BCL1D11S287E; CCND1; cyclin D1 (PRAD1: parathyroid adenomatosis 1); Cyclin D1; Cyl-1; G1/S-specific cyclin D1; G1/S-specific cyclin-D1; PRAD1 oncogene; PRAD1; PRAD1B-cell CLL/lymphoma 1; U21B31
Mass (kDA):
33.729 kDA
Human | |
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Location: | 11q13.3 |
Sequence: | 11; NC_000011.10 (69641156..69654474) |
Nucleus. Cytoplasm. Nucleus membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.
This article describes the CCND1 marker and the lncRNA in situ hybridization (ISH) procedure. This article will help you to choose the best method for your research needs. The CCND1 molecule can be used in many biological research techniques, such as RNA interference or genomic hybridization. Continue reading to find out more.
The CCND1 gene is present in human pancreatic tumors and is expressed by over 300 different cell types. This gene has several catalog numbers, including ab16663, cat. # 9308 and # 2211. # 2211. Anti-caspase-3 can be cat. # 9664; c-Myc is cat. # 2947.
One of the most widely used cancer diagnostic tests is the ISH procedure. The CCND1 gene encodes cyclin D1, a protein that promotes cell cycle progression. It has been associated with poor prognosis and ET resistance. It is not yet clear if this marker will be a good predictor in BCa.
There are several ways to detect CCND1 Amplification. Bright field ISH and CISH are preferred for CCND1 amplification detection. These are quicker and less costly than traditional methods.
This procedure can also help to determine if the tumour has amplification of CCND1. In a recent study, researchers found that CCND1 amplification was 9% to 57% in 6400 patient samples. 1135 (18%) amplified. This result is consistent in the CCND1 amplification described elsewhere. ISH analysis measures frequency of amplification in addition to CCND1 amplification.
Two blinded investigators assessed risk of bias by using a Covidence-designed form for quality assessment. Six domains of bias were evaluated using the Quality in Prognosis Studies Tool. These included outcome measurement, prognostic factor measurement and statistical analysis. However, some studies don't report this information. The RoB for all six domains also is low. This could bias the results in favor CCND1 survival.
ISH is a procedure where a complementary probe is used to detect a specific RNA or DNA sequence. It can be used on whole tissue, a cell or a sample. This procedure differs from immunohistochemistry, which uses specific antibodies to identify cell types or regions. ISH requires a high-sensitivity diagnostic kit, such is the one manufactured by Boster Bio-Engineering Company of Wuhan in China.
The company uses RNAscope(r), a technology that produces high-quality lncRNA (in situ hybridization) assays. These assays require only a few weeks to create and provide excellent sensitivity. ISH allows researchers to test a wide variety of lncRNAs, including those that regulate the expression of protein-coding genes. This technology has been widely used in the world of research to study and analyze lncRNAs.
The company's TCGA pan-cancer atlas is comprised of 33 different cancer types, and contains more than 9,560 cancer samples from each system. These samples were used in the generation of transcriptome data. They were also annotated with GENCODE Version 33. The resulting dataset included 10,656 different lncRNAs in all cancer types. There were also at least 20 normal samples that were close by. This data was used for differential screening.
Long non-codingRNAs are a new class of non-protein-coding RNA molecules. Their lengths extend beyond 200 bp. They are home to a large variety of non-coding transcripts that are highly related to cellular processes. Additionally, long noncodingRNAs are involved in multiple cellular processes including tumor development. Because of these, it is essential to characterize long noncoding RNAs and their function in tumorigenesis.
PMID: 1826542 by Motokura T., et al. A novel cyclin encoded by a bcl1-linked candidate oncogene.
PMID: 1833066 by Lew D.J., et al. Isolation of three novel human cyclins by rescue of G1 cyclin (Cln) function in yeast.
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