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- Table of Contents
CCND1 antibody (M00149-1) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 27 Publications
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Immunofluorescent analysis of MCF-7 cells, using Cyclin D1 Antibody.
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Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody
CCND1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
CCND1 antibody was positively detected by WB in:
human MCF-7 whole cell, human LNCAP whole cell, human HepG2 whole cell, human A431 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CCND1 antibody was positively detected by IHC in:
human bladder tissue, Human pituitary tumor tissue, Human squamous cell carcinoma tissue, Human ovarian cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
Human
Tested
Tested
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody
View all CCND1 Antibodies
View all CCND1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DAD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cyclin D1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
34 kDa
Calculated Molecular Weight
33.7 kDa
Cite This Product
Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00149-1)
Protocols
Western Blot protocol for CCND1 antibody
Immunohistochemistry (IHC) protocol for CCND1 antibody
Publications
Application:
Species:
ERK promotes miR34a/ISOC1 signaling to enhance all trans retinoic acid-induced myeloid differentiation in acute promyelocytic leukemia
Published: 2026-06-20
Journal: JOURNAL OF BIOLOGICAL CHEMISTRY
Application: WB
Impact Factor: 4.10
Species: Human
PMID: 42323085
A Specific tRNA Half, 3'tiRNA-GlyGCC, Regulates Hypoxic Pulmonary Artery Smooth Muscle Cell Proliferation via Myrf-Mediated Endoplasmic Reticulum Stress
Published: 2026-05-29
Journal: CELL PROLIFERATION
Application: WB
Impact Factor: 7.60
Species: Mouse
PMID: 42215285
Syncytin-a deficiency compromises murine sperm function by suppressing PRL/PGE2 and PI3K/AKT/mTOR pathway
Published: 2026-04-10
Journal: iScience
Application: WB
Impact Factor: 4.10
Species: Mouse
PMID: 42095086
Super enhancer-driven LINC01013 mediates hypoxia-induced mitochondrial dysfunction by HSPA9 to determine pulmonary arterial smooth muscle cell fate
Published: 2026-01-06
Journal: CELLULAR AND MOLECULAR LIFE SCIENCES
Application: WB
Impact Factor: 6.20
Species: Human
PMID: 41491061
Predictive and personalized approaches for idiopathic pulmonary fibrosis: a Wnt-related gene set scoring framework integrating single-cell sequencing, spatial transcriptomics, and machine learning for diagnosis and prognosis
Published: 2025-03-13
Journal: FUNCTIONAL & INTEGRATIVE GENOMICS
Application: IF
Impact Factor: 3.10
Species: Human
PMID: 40080215
B-doped nano-hydroxyapatite facilitates proliferation and differentiation of osteoblasts
Published: 2025-01-18
Journal: Journal of Orthopaedic Surgery and Research
Application: WB
Impact Factor: 2.80
Species: Human
PMID: 39825395
MAT1A activation of glycolysis to promote NSCLC progression depends on stabilizing CCND1
Published: 2024-10-22
Journal: Cell Death & Disease
Application: IHC
Impact Factor: 8.10
Species: Mouse
PMID: 39438468
Andrographolide inhibits the proliferation and migration of vascular smooth muscle cells via PI3K/AKT signaling pathway and amino acid metabolism to prevent intimal hyperplasia
Published: 2023-09-30
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 5.00
Species: Rat
PMID: 37783303
Naringenin suppresses epithelial ovarian cancer by inhibiting proliferation and modulating gut microbiota
Published: 2022-08-19
Journal: PHYTOMEDICINE
Application: IHC,WB
Impact Factor: 6.66
Species: Human,Mouse
PMID: 36029647
Magnolol alleviates hypoxia-induced pulmonary vascular remodeling through inhibition of phenotypic transformation in pulmonary arterial smooth muscle cells
Published: 2022-05-06
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB
Impact Factor: 7.42
Species: Rat
PMID: 35658230
Nuciferine Inhibits TMEM16A in Dietary Adjuvant Therapy for Lung Cancer
Published: 2022-03-17
Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Application: WB
Impact Factor: 5.90
Species: Mouse
PMID: 35298888
SIRT6 Promotes the Progression of Prostate Cancer via Regulating the Wnt/β-Catenin Signaling Pathway
Published: 2022-02-25
Journal: Journal of Oncology
Application: WB
Impact Factor: 4.50
Species: Human
PMID: 35251169
Sdy-1 Executes Antitumor Activity in HepG2 and HeLa Cancer Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway
Published: 2022-02-05
Journal: Marine Drugs
Application: WB
Impact Factor: 6.09
Species: Human
PMID: 35200654
Ubiquitinated AIF is a major mediator of hypoxia-induced mitochondrial dysfunction and pulmonary artery smooth muscle cell proliferation
Published: 2022-01-28
Journal: Cell and Bioscience
Application: WB
Impact Factor: 9.58
Species: Rat
PMID: 35090552
piRNA-63076 contributes to pulmonary arterial smooth muscle cell proliferation through acyl-CoA dehydrogenase
Published: 2020-03-30
Journal: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Application: WB
Impact Factor: 4.49
Species: Rat
PMID: 32227582
Antitumor Effect of miR-1294/Pyruvate Kinase M2 Signaling Cascade in Osteosarcoma Cells
Published: 2020-02-21
Journal: OncoTargets and Therapy
Application: WB,IHC
Impact Factor: 3.34
Species: Human
PMID: 32110059
Downregulation of long non‑coding RNA ANRIL promotes proliferation and migration in hypoxic human pulmonary artery smooth muscle cells
Published: 2019-12-17
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 1.85
Species: Human
PMID: 31974617
Macelignan inhibits the inflammatory response of microglia and regulates neuronal survival
Published: 2019-12-10
Journal: JOURNAL OF NEUROIMMUNOLOGY
Application: IF
Impact Factor: 2.83
Species: Human
PMID: 31838278
ZEB1 regulates the activation of hepatic stellate cells through Wnt/β-catenin signaling pathway
Published: 2019-11-08
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 3.17
Species: Human
PMID: 31712061
Interaction of Follicle-Stimulating Hormone and Stem Cell Factor to Promote Primordial Follicle Assembly in the Chicken
Published: 2019-02-19
Journal: Frontiers in Endocrinology
Application: IHC,WB
Impact Factor: 3.63
Species: Chicken
PMID: 30837955
CD38 affects the biological behavior and energy metabolism of nasopharyngeal carcinoma cells
Published: 2018-12-03
Journal: INTERNATIONAL JOURNAL OF ONCOLOGY
Application: WB
Impact Factor: 3.33
Species: Human
PMID: 30535454
RSPO2 enhances cell invasion and migration via the WNT/β-catenin pathway in human gastric cancer
Published: 2018-10-26
Journal: JOURNAL OF CELLULAR BIOCHEMISTRY
Application: IF
Impact Factor: 2.96
Species: Human
PMID: 30362605
Sohlh1 suppresses glioblastoma cell proliferation, migration, and invasion by inhibition of Wnt/β-catenin signaling
Published: 2017-12-14
Journal: MOLECULAR CARCINOGENESIS
Application: WB
Impact Factor: 4.19
Species: Mouse
PMID: 29240260
Evodiamine promotes differentiation and inhibits proliferation of C2C12 muscle cells
Published: 2017-12-13
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: WB
Impact Factor: 2.34
Species: Mouse
PMID: 29286060
RASSF1A promotes apoptosis and suppresses the proliferation of ovarian cancer cells
Published: 2014-02-25
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: WB
Impact Factor: 1.88
Species: Human
PMID: 24573512
Molecular mechanism of inhibitory effects of C-phycocyanin combined with all-trans-retinoic acid on the growth of HeLa cells in vitro
Published: 2014-02-23
Journal: TUMOR BIOLOGY
Application: IF
Impact Factor: 2.84
Species: Human
PMID: 24563337
Apoptotic effects of psiRNA-STAT3 on 4T1 breast cancer cells in vitro.
Published: 2014-01-01
Journal: Asian Pacific Journal of Cancer Prevention
Application: IHC
Impact Factor: 1.50
Species: Mouse
PMID: 25169471
Review
Have you used Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody?
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1 Review For Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody
This antibody exhibits high efficiency and specificity and is suitable for Western blot detection of CCND1 protein in rat colon tissue, with only minor nonspecific bands observed.
| SKU | M00149-1 |
|---|---|
| Application | Western Blot |
| Sample | rat colon tissue |
| Sample Processing Description | RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE. |
| Other Reagents | Blocking buffer |
| Primary Antibody | Cyclin D1 CCND1 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) |
| Secondary Incubation | 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | The figure shows the Western blot results of the target protein CCND1 and the internal control Actin in rat colon tissue across the following groups: normal, disease model, low/middle/high dose traditional Chinese medicine treatment, and Western medicine treatment. The target bands are clear and distinct, and the experimental results are satisfactory. |
Shiyu Zhang, LUTCM
Verified customer
Submitted 2026-01-06
Product Promise
We promise Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00149-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026