Product Info Summary
SKU: | M00149-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00149-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody catalog # M00149-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00149-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DAD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cyclin D1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00149-1 is reactive to CCND1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
34 kDa
Calculated molecular weight
33729 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00149-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:100
IP 1:50
Positive Control
WB: human MCF-7 whole cell, human LNCAP whole cell, human HepG2 whole cell, human A431 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue
IHC: human bladder tissue, Human pituitary tumor tissue, Human squamous cell carcinoma tissue, Human ovarian cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Cyclin D1 using anti-Cyclin D1 antibody (M00149-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human LNCAP whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin D1 antigen affinity purified monoclonal antibody (Catalog # M00149-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin D1 at approximately 34 kDa. The expected band size for Cyclin D1 is at 34 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human bladder, using Cyclin D1 Antibody.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:100 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma , using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human ovarian cancer, using the Antibody at 1:500 dilution.
Click image to see more details
Immunofluorescent analysis of MCF-7 cells, using Cyclin D1 Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CCND1 (Source: Uniprot.org, NCBI)
Gene Name
CCND1
Full Name
G1/S-specific cyclin-D1
Weight
33729 MW
Superfamily
cyclin family
Alternative Names
G1/S-specific cyclin-D1;B-cell lymphoma 1 protein;BCL-1;BCL-1 oncogene;PRAD1 oncogene;CCND1;BCL1, PRAD1; CCND1 BCL1, D11S287E, PRAD1, U21B31 cyclin D1 G1/S-specific cyclin-D1|B-cell CLL/lymphoma 1|B-cell lymphoma 1 protein|BCL-1 oncogene|PRAD1 oncogene
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCND1, check out the CCND1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCND1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody (M00149-1)
Hello CJ!
M00149-1 has been cited in 27 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen Q, Deng X,Hu X,Guan S,He M,Wang Y,Wei B,Zhang J,Zhao H,Yao W,Jin F,Liu Y,Chen J,Olapade OI,Wu H,Wei M.Breast Cancer Risk-Associated SNPs in the mTOR Promoter Form De Novo KLF5- and ZEB1-Binding Sites that Influence the Cellular Response to Paclitaxel.Mol Cancer Res.2019 Nov;17(11):2244-2256.doi:10.1158/1541-7786.MCR-18-1072.Epub 2019 Aug 29.PMID:31467112.
Species: Human,Mouse
M00149-1 usage in article: APP:WB, SAMPLE:MCF-7 CELL, DILUTION:1:1000
Xiang W,Jiang T,Hao X, Wang R,Yao X,Sun K,Guo F,Xu T.Primary cilia and autophagy interaction is involved in mechanical stress mediated cartilage development via ERK/mTOR axis.Life Sci.2019 Feb 1;218:308-313.doi:10.1016/j. lfs.2019.01.001.Epub 2019 Jan 3.PMID:30610869.
Species: Rat
M00149-1 usage in article: APP: WB, SAMPLE:CHONDROCYTES, DILUTION:1:100
Zhang H,Su Y,Wang J,Gao Y,Yang F,Li G,Shi Q.Ginsenoside Rb1 promotes the growth of mink hair follicle via PI3K/AKT/GSK-3β signaling pathway.Life Sci.2019 Jul 15;229:210-218.doi:10.1016/j.lfs.2019.05.033.Epub 2019 May 16.PMID:31102746.
Species: American Minks
M00149-1 usage in article: APP:WB, SAMPLE:HAIR FOLLICLES AND DPCS, DILUTION:1:400
Liu S,Sun Y,Zhao R,Wang Y,Zhang W,Pang W. Isoleucine increases muscle mass through promoting myogenesis and intramyocellular fat deposition. Food Funct.2021 Jan 7;12(1):144-153.doi: 10.1039/d0fo02156c.Epub 2020 Dec 8. PMID:33289736.
Species: Mouse
M00149-1 usage in article: APP:WB, SAMPLE:MUSCLE TISSUE AND C2C12 CELLS, DILUTION:1:500
Wang S,Zhang C,Zhang X.Downregulation of long non‑coding RNA ANRIL promotes proliferation and migration in hypoxic human pulmonary artery smooth muscle cells.Mol Med Rep.2020 Feb;21(2):589-596.doi:10.3892/mmr.2019.10887.Epub 2019 Dec 17.PMID:31974617; PMC
Species: Human
M00149-1 usage in article: APP:WB, SAMPLE:HPASMCS, DILUTION:1:200
Wang C,Shao S,Deng L,Wang S,Zhang Y. LncRNA SNHG12 regulates the radiosensitivity of cervical cancer through the miR-148a/CDK1 pathway. Cancer Cell Int.2020 Dec 1;20(1):554.doi:10.1186/s12935-020-01654-5.PMID:33292254;PMCID:PMC7708190.
Species: Human
M00149-1 usage in article: APP:WB, SAMPLE:SIHA AND HELA CELLS, DILUTION:1:2000
Effects of Buddleja officinalis granules on apoptosis factors Bax, Caspase-3, Fas, and FasL in lacrimal gland cells of castrated male rabbits Genyan Qin, et al. J Ophthalmol. 2019 Feb 5;2019:5916243. doi: 10.1155/2019/5916243. eCollection 2019.
Effects of xanthohumol-rich hop extract on the differentiation of preadipocytes
Overexpression of MYCT1 inhibits proliferation and induces apoptosis in human acute myeloid leukemia HL-60 and KG-1a cells in vitro and in vivo
Sirt1 AS lncRNA interacts with its mRNA to inhibit muscle formation by attenuating function of miR-34a
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Customer Q&As
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1 Customer Q&As for Anti-Cyclin D1 CCND1 Rabbit Monoclonal Antibody
Question
Could you ask Abways for the immunogen sequence of M00149-1 (CY5404) Cyclin D1?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
The immunogen sequence of M00149-1 is VDLACTPTDVRDVDI.
Boster Scientific Support
Answered: 2020-04-30