Rabbit IgG polyclonal antibody for Cytochrome P450 11B1, mitochondrial(CYP11B1) detection. Tested with WB in Human.
Anti-CYP11B1 Antibody Info At A Glance
|Product Name||Anti-CYP11B1 Antibody
See all CYP11B1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for CYP11B1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. CYP11B1 information: Molecular Weight: 57573 MW; Subcellular Localization: Mitochondrion membrane.|
|Cite This Product||Anti-CYP11B1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1699)|
|Similar Products From
|Anti-CYP11B1 Antibody may replace the following items: sc 47647|sc 47649|sc 374096|sc 47652|sc 377248|sc 377401.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human CYP11B1(436-450aa RNFYHVPFGFGMRQC).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For CYP11B1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||1584|
|Species Of This Entry||Human|
|Protein Name||Cytochrome P450 11B1, mitochondrial|
|Superfamily||cytochrome P450 family|
|Alternative Names||CYP11B1|CPN1; CYPXIB1; cytochrome P450 11B1, mitochondrial; cytochrome p450 XIB1; cytochrome P450, family 11, subfamily B, polypeptide 1; cytochrome P450, subfamily XIB (steroid 11-beta-hydroxylase), polypeptide 1; Cytochrome P450C11; Cytochrome P-450c11; EC 1.14.15; EC 126.96.36.199; FHICYP11B; FLJ36771; P450C11DKFZp686B05283; S11BH; Steroid 11-beta-hydroxylase; steroid 11-beta-monooxygenase|
|Post Tranlational Modifications||Hydroxylation; Cleavage; Phosphorylation; Reduction; Sumoylation; Acetylation; Oxidation; Dephosphorylation|
|Subcellular Localization||Mitochondrion inner membrane; Peripheral membrane protein.|
|Gene Location||8q24.3, on Chromasome 8, gene sequence: NC_000008.11 (142872357..142879825, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CYP11B1 (Source: Uniprot.org, NCBI)
|Protein Function||Has steroid 11-beta-hydroxylase activity. In addition to this activity, the 18 or 19-hydroxylation of steroids and the aromatization of androstendione to estrone have also been ascribed to cytochrome P450 XIB.|
|Research Areas||Lipid and Metabolism|
|Related Diseases||Hypertensive Disease; Hyperplasia; Congenital Adrenal Hyperplasia; Adrenal Hyperplasia; Hyperaldosteronism; Conn Syndrome; Adenoma; Neoplasms; Tumors Of Adrenal Cortex; Chronic Active Hepatitis; 11-beta-hydroxylase Deficiency; Heart Failure; Essential Hypertension; Adrenal Cortical Adenoma; Cushing Syndrome; Fibrosis; Virilism; Adrenal Gland Neoplasms; Malignant Neoplasm Of Adrenal Cortex; Malignant Neoplasms|
|Related Pathways||Secretion; Aldosterone Secretion; Excretion; Cortisol Secretion; Gene Conversion; Localization; Transport; Pathogenesis; Electron Transport; Water Homeostasis; Cell Proliferation; Reverse Transcription; Cholesterol Transport; Hormone Secretion; Cell Death; Steroid Hormone Secretion; Spermatogenesis; Response To Stress; Ovulation; Regeneration|
|Background||CYP11B1(Cytochrome p450, family 11, subfamily B, polypeptide 1), also called Steroid 11-beta-hydroxylase or P450C11, is a steroid hydroxylase found in the zona fasciculata. The CYP11B1 gene functions primarily in mitochondria in the zona fasciculata of the adrenal cortex to convert 11-deoxycortisol to cortisol and 11-deoxycorticosterone to corticosterone. CYP11B1 is a member of the cytochrome P450 superfamily of enzymes. It is mapped on 8q24.3. The CYP11B1 gene contains 9 exons and spans 6.5 kb. Using RT-PCR, Kayes-Wandover and White detected CYP11B1 mRNA in human cardiac tissue samples from left and right atria, aorta, apex, intraventricular septum, and atrioventricular node, as well as whole adult and fetal heart. Ventricles did not express CYP11B1. In patients with 11-beta-hydroxylase deficiency leading to hypertension and congenital adrenal hyperplasia, Pascoe et al. identified mutations in the CYP11B1 gene.|
|Scientific References||PMID: 2592361 by Mornet E., et al. Characterization of two genes encoding human steroid 11 beta- hydroxylase (P-450(11) beta).|
PMID: 2401360 by Kawamoto T., et al. Cloning of cDNA and genomic DNA for human cytochrome P-45011 beta.
Our Boster Quality Guarantee for Anti-CYP11B1 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti CYP11B1 (PA1699) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Lane 5: MM453 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 58KD
Observed bind size: 58KD
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Note: sample size takes longer to process, average 5-7 days.
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