|Product Name||Anti-Cytochrome C/CYCS Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Cytochrome c(CYCS) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-Cytochrome C/CYCS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1118)|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Cytochrome C(91-105 aa ERADLIAYLKKATNE), identical to the related mouse and rat sequences.|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, Rat, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
Images And Assay Conditions
Anti-Cytochrome C antibody, PA1118, IHC(P)
IHC(P): Rat Lung Tissue
Anti-Cytochrome C antibody, PA1118, IHC(F)
IHC(F): Rat Brain Tissue
Anti-Cytochrome C antibody, PA1118, ICC
ICC: A549 Cell
Anti-Cytochrome C antibody, PA1118, Western blotting
All lanes: Anti Cytochrome C (PA1118) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 12KD
Observed bind size: 12KD
Protein Target Info (Source: Uniprot.org)
|Protein Name||Cytochrome c|
|Alternative Names||Cytochrome c;CYCS;CYC;|
|Subcellular Localization||Mitochondrion intermembrane space. Loosely associated with the inner membrane.|
|Molecular Weight||11749 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.|
|Research Areas||Apoptosis, Apoptotic Markers, Cancer, Cancer Metabolism, Cardiovascular, Cell Biology, Cell Death, Cytochromes, Energy Metabolism, Energy Transfer Pathways, Integration Of Energy, Integration Of Energy Metabolism, Invasion/Microenvironment, Lipases, Lipid And Lipoprotein Metabolism, Lipid Metabolism, Lipids / Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Processes, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Oxidative Phosphorylation, Pathways And Processes, Signal Transduction
*You can search these to find other products in these research areas.
|Background||Cytochrome C is located in the mitochondria of all aerobic cells and is involved in the electron transport system. Human cytochrome c has 104 amino acid residues and a molecular weight of 11,458 and is mapped to 7p15.2. Cytochrome c released from mitochondria has been proposed to be an essential component of an apoptotic pathway responsive to DNA damage and other forms of cell stress. And it has a role in different apoptotic signaling cascades.|
Other Recommended Resources
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1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,