Anti-Cytochrome C/CYCS Antibody

Boster Bio Anti-Cytochrome C/CYCS Antibody catalog # PA1118. Tested in Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. Cited in 19 publication(s).

Product Info Summary

SKU: PA1118
Size: 100μg/vial
Reactive Species: Human, Monkey, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB

Product Name

Anti-Cytochrome C/CYCS Antibody

See all Cytochrome c products

SKU/Catalog Number

PA1118

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cytochrome C/CYCS Antibody catalog # PA1118. Tested in Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cytochrome C/CYCS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1118)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Cytochrome C (91-105 aa ERADLIAYLKKATNE), identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1118 is reactive to CYCS in Human, Monkey, Mouse, Rat

Applications

PA1118 is guaranteed for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Rat, Mouse
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For CYCS (Source: Uniprot.Org, NCBI)

Uniprot ID

P99999

Gene ID

54205

Gene Name

CYCS

Full Name

Cytochrome c

Weight

11.749kDa

Superfamily

cytochrome c family

Alternative Names

CYCHCS; CYCS; Cytochrome c; cytochrome c, somatic; THC4 CYCS CYC, HCS, THC4 cytochrome c, somatic cytochrome c

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CYCS, check out the CYCS Infographic

CYCS infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CYCS: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1118 has been cited in 19 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhang T,Chen Y,Cai J,Pan M,Sun Q,Zhang J,Sun C.SOCS2 Inhibits Mitochondrial Fatty Acid Oxidation via Suppressing LepR/JAK2/AMPK Signaling Pathway in Mouse Adipocytes.Oxid Med Cell Longev.2020 Jul 13;2020:3742542.doi:10.1155/2020/3742542.PMID:32733634;PMCI
Species: Mouse
PA1118 usage in article: APP:IF, SAMPLE:ADIPOCYTES, DILUTION:1:100

Neuroprotective Effect of Tea Polyphenols on Oxyhemoglobin Induced Subarachnoid Hemorrhage in Mice

Chinese herbal medicine Yougui Pill reduces exogenous glucocorticoid-induced apoptosis in anterior pituitary cells

Catalpol inhibits apoptosis in hydrogen peroxide-induced cardiac myocytes through a mitochondrial-dependent caspase pathway

Methacryloxylethyl Cetyl Ammonium Chloride Induces DNA Damage and Apoptosis in Human Dental Pulp Cells via Generation of Oxidative Stress

N-Acetyl Cysteine Depletes Reactive Oxygen Species and Prevents Dental Monomer-Induced Intrinsic Mitochondrial Apoptosis In Vitro in Human Dental Pulp Cells

HOXB1 Is a Tumor Suppressor Gene Regulated by miR-3175 in Glioma

FABP4 reversed the regulation of leptin on mitochondrial fatty acid oxidation in mice adipocytes

Lin W, Tongyi S. Tumour Biol. 2014 Aug;35(8):8065-75. Doi: 10.1007/S13277-014-2064-0. Epub 2014 May 19. Role Of Bax/Bcl-2 Family Members In Green Tea Polyphenol Induced Necroptosis Of P53-Deficient Hep3B Cells.

Zhang W, Liu N, Wang X, Jin X, Du H, Peng G, Xue J. Hum Exp Toxicol. 2015 Feb;34(2):179-90. Doi: 10.1177/0960327114533358. Epub 2014 May 16. Benzo(A)Pyrene-7,8-Diol-9,10-Epoxide Induced P53-Independent Necrosis Via The Mitochondria-Associated Path...

Li Wz, Li Wp, Huang Dk, Kan Hw, Wang X, Wu Wy, Yin Yy, Yao Yy. Behav Brain Res. 2012 Feb 1;227(1):142-9. Doi: 10.1016/J.Bbr.2011.10.038. Epub 2011 Oct 29. Dexamethasone And A????????-?????? Accelerate Learning And Memory Impairments Due To Elevate...

Huang C, Lin Y, Su H, Ye D. Neurochem Res. 2015 Jan;40(1):27-35. Doi: 10.1007/S11064-014-1461-5. Epub 2014 Oct 25. Forsythiaside Protects Against Hydrogen Peroxide-Induced Oxidative Stress And Apoptosis In Pc12 Cell.

Ban Jb, Fan Xw, Huang Q, Li Bf, Chen C, Zhang Hc, Xu Sq. Plos One. 2014 May 16;9(5):E97607. Doi: 10.1371/Journal.Pone.0097607. Ecollection 2014. Mono-(2-Ethylhexyl) Phthalate Induces Injury In Human Umbilical Vein Endothelial Cells.

Li X, Wang L, Wang Z. Phys Med. 2015 Jun;31(4):352-9. Doi: 10.1016/J.Ejmp.2015.02.004. Epub 2015 Feb 19. Radioprotective Activity Of Neutral Polysaccharides Isolated From The Fruiting Bodies Of Hohenbuehelia Serotina.

Wei T, Tian W, Yan H, Shao G, Xie G. Cell Mol Neurobiol. 2014 Nov;34(8):1165-73. Doi: 10.1007/S10571-014-0091-4. Epub 2014 Aug 22. Protective Effects Of Phillyrin On H2O 2-Induced Oxidative Stress And Apoptosis In Pc12 Cells.

Ding H, Wang H, Zhao Y, Sun D, Zhai X. Cell Mol Neurobiol. 2015 Jul;35(5):623-32. Doi: 10.1007/S10571-015-0156-Z. Epub 2015 Jan 18. Protective Effects Of Baicalin On A??????????????-Induced Learning And Memory Deficit, Oxidative Stress, And Apopto...

Xu S, Liu J, Zhang Y, Wang C, Wang J, Yang Y, Huo J, Sun W. Neural Regen Res. 2012 Jun 15;7(17):1318-24. Doi: 10.3969/J.Issn.1673-5374.2012.17.006. Apoptosis-Related Protein Expression In Rabbits With Blast Brain Injury Following Early Hyperbaric ...

Song D, Yue W, Li Z, Li J, Zhao J, Zhang N. Onco Targets Ther. 2014 Sep 30;7:1801-10. Doi: 10.2147/Ott.S52426. Ecollection 2014. Study Of The Mechanism Of Sonodynamic Therapy In A Rat Glioma Model.

Jiang H, Li J, Zhou T, Wang C, Zhang H, Wang H. Int J Mol Med. 2014 May;33(5):1298-304. Doi: 10.3892/Ijmm.2014.1684. Epub 2014 Mar 6. Colistin-Induced Apoptosis In Pc12 Cells: Involvement Of The Mitochondrial Apoptotic And Death Receptor Pathways.

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16 Customer Q&As for Anti-Cytochrome C/CYCS Antibody

Question

My question regarding product PA1118, anti-Cytochrome C/CYCS antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

A. Carter

Verified customer

Asked: 2020-01-14

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1118 anti-Cytochrome C/CYCS antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-14

Question

Our lab used your anti-Cytochrome C/CYCS antibody for IHC-P on bone marrow last year. I am using mouse, and We are going to use the antibody for WB next. you antibody examining bone marrow as well as amygdala in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-11-26

Answer

I have checked the website and datasheets of our anti-Cytochrome C/CYCS antibody and it appears that PA1118 has been validated on mouse in both IHC-P and WB. Thus PA1118 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-11-26

Question

Does anti-Cytochrome C/CYCS antibody PA1118 work for ICC with kidney?

E. Dhar

Verified customer

Asked: 2019-11-25

Answer

According to the expression profile of kidney, CYCS is highly expressed in kidney. So, it is likely that anti-Cytochrome C/CYCS antibody PA1118 will work for ICC with kidney.

Boster Scientific Support

Answered: 2019-11-25

Question

Is this PA1118 anti-Cytochrome C/CYCS antibody reactive to the isotypes of CYCS?

Verified Customer

Verified customer

Asked: 2019-10-18

Answer

The immunogen of PA1118 anti-Cytochrome C/CYCS antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Cytochrome C(91-105 aa ERADLIAYLKKATNE), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-10-18

Question

We need using your anti-Cytochrome C/CYCS antibody for mitochondrial electron transport studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-08-30

Answer

Thanks for your inquiry. This PA1118 anti-Cytochrome C/CYCS antibody is validated on rat lung tissue, tissue lysate, brain tissue, liver tissue, hela whole cell lysate, hepa whole cell lysate. It is guaranteed to work for IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-08-30

Question

My team were happy with the WB result of your anti-Cytochrome C/CYCS antibody. However we have been able to see positive staining in skin, rc testis urinary bladder mitochondrion intermembrane space. using this antibody. Is that expected? Could you tell me where is CYCS supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-08-13

Answer

From what I have seen in literature, skin, rc testis urinary bladder does express CYCS. Generally CYCS expresses in mitochondrion intermembrane space. Regarding which tissues have CYCS expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Bone marrow, Brain, Kidney, Lung, Skeletal muscle, Skin, Testis, and Urinary bladder, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 13933734, 14063298, 9515723
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-08-13

Question

Do you have a BSA free version of anti-Cytochrome C/CYCS antibody PA1118 available?

Verified Customer

Verified customer

Asked: 2018-12-03

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cytochrome C/CYCS antibody PA1118 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-12-03

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-Cytochrome C/CYCS antibody PA1118. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-09-14

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-09-14

Question

Is a blocking peptide available for product anti-Cytochrome C/CYCS antibody (PA1118)?

T. Collins

Verified customer

Asked: 2018-09-04

Answer

We do provide the blocking peptide for product anti-Cytochrome C/CYCS antibody (PA1118). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-09-04

Question

I was wanting to use your anti-Cytochrome C/CYCS antibody for ICC for mouse kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse kidney identification?

Verified Customer

Verified customer

Asked: 2018-06-19

Answer

It shows on the product datasheet, PA1118 anti-Cytochrome C/CYCS antibody has been tested for IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse kidney in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-06-19

Question

We have been able to see staining in mouse liver. Are there any suggestions? Is anti-Cytochrome C/CYCS antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2018-01-09

Answer

Based on literature liver does express CYCS. Based on Uniprot.org, CYCS is expressed in heart, cerebellum, amygdala, bone marrow, brain, kidney, lung, skeletal muscle, skin, rc testis urinary bladder, erythroleukemia, liver, among other tissues. Regarding which tissues have CYCS expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Bone marrow, Brain, Kidney, Lung, Skeletal muscle, Skin, Testis, and Urinary bladder, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 13933734, 14063298, 9515723
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2018-01-09

Question

We are currently using anti-Cytochrome C/CYCS antibody PA1118 for human tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

P. Parker

Verified customer

Asked: 2017-04-24

Answer

The anti-Cytochrome C/CYCS antibody (PA1118) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-04-24

Question

Please see the WB image, lot number and protocol we used for kidney using anti-Cytochrome C/CYCS antibody PA1118. Please let me know if you require anything else.

K. Carter

Verified customer

Asked: 2016-10-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-10-13

Question

Would PA1118 anti-Cytochrome C/CYCS antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

N. Li

Verified customer

Asked: 2016-03-21

Answer

It shows on the product datasheet, PA1118 anti-Cytochrome C/CYCS antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-03-21

Question

you antibody to test anti-Cytochrome C/CYCS antibody PA1118 on mouse kidney for research purposes, then I may be interested in using anti-Cytochrome C/CYCS antibody PA1118 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

A. Zhao

Verified customer

Asked: 2015-10-23

Answer

The products we sell, including anti-Cytochrome C/CYCS antibody PA1118, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-10-23

Question

I see that the anti-Cytochrome C/CYCS antibody PA1118 works with ICC, what is the protocol used to produce the result images on the product page?

L. Wu

Verified customer

Asked: 2013-04-24

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-04-24

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PA1118
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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