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Product Info Summary
| SKU: | PB9455 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-VDAC/Porin/VDAC1 Antibody Picoband®
SKU/Catalog Number
PB9455
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VDAC/Porin/VDAC1 Antibody Picoband® catalog # PB9455. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VDAC/Porin/VDAC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9455)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human VDAC/Porin, different from the related mouse and rat sequences by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9455 is reactive to VDAC1 in Human, Mouse, Rat
Observed Molecular Weight
35 kDa
Calculated molecular weight
30.8 kDa
Background of VDAC1
The voltage-dependent anion channel (VDAC) of the outer mitochondrial membrane is a small, abundant outer membrane pore-forming protein found in the outer membranes of all eukaryotic mitochondria. The VDAC protein is thought to form the major pathway for movement of adenine nucleotides through the outer membrane and to be the mitochondrial binding site for hexokinase and glycerol kinase. At low transmembrane voltage, VDAC is open for anions such as phosphate, chloride, and adenine nucleotides. At higher transmembrane voltage, VDAC functions as a selective channel for cations and uncharged molecules. These features make VDAC likely to play a role in mitochondrial energy metabolism. Huizing et al. studied by Northern and Western blot analyses the human tissue distribution of mitochondrial transmembrane metabolite carriers. They found that VDAC1 mRNA has a ubiquitous distribution, with most pronounced expression in heart, liver, and skeletal muscle, whereas the VDAC2 isoform appears to be expressed only in the heart.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9455 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human Caco-2 whole cell, human A549 whole cell, human 293T whole cell, human HepG2 whole cell, rat heart tissue, rat kidney tissue, mouse heart tissue, mouse kidney tissue
IHC: mouse cardiac muscle tissue, rat cardiac muscle tissue, human intestinal cancer tissue
Validation Images & Assay Conditions
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IHC analysis of VDAC using anti-VDAC antibody (PB9455).
VDAC was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VDAC Antibody (PB9455) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Western blot analysis of VDAC using anti-VDAC antibody (PB9455).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VDAC antigen affinity purified polyclonal antibody (Catalog # PB9455) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VDAC at approximately 35 kDa. The expected band size for VDAC is at 31 kDa.
Click image to see more details
IHC analysis of VDAC using anti-VDAC antibody (PB9455).
VDAC was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VDAC Antibody (PB9455) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of VDAC using anti-VDAC antibody (PB9455).
VDAC was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VDAC Antibody (PB9455) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-VDAC/Porin/VDAC1 Antibody Picoband® (PB9455)
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Customer Q&As
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16 Customer Q&As for Anti-VDAC/Porin/VDAC1 Antibody Picoband®
Question
Is a blocking peptide available for product anti-VDAC/Porin/VDAC1 antibody (PB9455)?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
We do provide the blocking peptide for product anti-VDAC/Porin/VDAC1 antibody (PB9455). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-14
Question
My question regards using your anti-VDAC/Porin/VDAC1 antibody for pyruvate metabolic process studies. Has this antibody been tested with western blotting on cardiac muscle tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
I appreciate your inquiry. This PB9455 anti-VDAC/Porin/VDAC1 antibody is validated on rat liver tissue, tissue lysate, mouse liver tissue, smmc whole cell lysate, cardiac muscle tissue, intestinal cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-10
Question
Will PB9455 anti-VDAC/Porin/VDAC1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
It shows on the product datasheet, PB9455 anti-VDAC/Porin/VDAC1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-31
Question
We are currently using anti-VDAC/Porin/VDAC1 antibody PB9455 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
The anti-VDAC/Porin/VDAC1 antibody (PB9455) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-06
Question
I see that the anti-VDAC/Porin/VDAC1 antibody PB9455 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-03
Question
Is this PB9455 anti-VDAC/Porin/VDAC1 antibody reactive to the isotypes of VDAC1?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
The immunogen of PB9455 anti-VDAC/Porin/VDAC1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human VDAC/Porin (154-181aa QMNFETAKSRVTQSNFAVGYKTDEFQLH), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-08
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-VDAC/Porin/VDAC1 antibody PB9455. Let me know if you need anything else.
A. Patel
Verified customer
Asked: 2019-06-10
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-10
Question
We have tried in the past anti-VDAC/Porin/VDAC1 antibody for WB on cervix carcinoma erythroleukemia a few months ago. I am using mouse, and We want to use the antibody for IHC next. I was wanting to use examining cervix carcinoma erythroleukemia as well as b-cell lymphoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
I have checked the website and datasheets of our anti-VDAC/Porin/VDAC1 antibody and it seems that PB9455 has been tested on mouse in both WB and IHC. Thus PB9455 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-03
Question
My team were content with the WB result of your anti-VDAC/Porin/VDAC1 antibody. However we have been able to see positive staining in skeletal muscle mitochondrion outer membrane using this antibody. Is that expected? Could you tell me where is VDAC1 supposed to be expressed?
A. Mangal
Verified customer
Asked: 2019-03-14
Answer
Based on literature, skeletal muscle does express VDAC1. Generally VDAC1 expresses in mitochondrion outer membrane. Regarding which tissues have VDAC1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 7685903
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Skeletal muscle, Pubmed ID: 1657034
Boster Scientific Support
Answered: 2019-03-14
Question
Would anti-VDAC/Porin/VDAC1 antibody PB9455 work for IHC with brain?
Verified Customer
Verified customer
Asked: 2019-02-11
Answer
According to the expression profile of brain, VDAC1 is highly expressed in brain. So, it is likely that anti-VDAC/Porin/VDAC1 antibody PB9455 will work for IHC with brain.
Boster Scientific Support
Answered: 2019-02-11
Question
Do you have a BSA free version of anti-VDAC/Porin/VDAC1 antibody PB9455 available?
Verified Customer
Verified customer
Asked: 2019-01-08
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-VDAC/Porin/VDAC1 antibody PB9455 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-08
Question
I was wanting to use your anti-VDAC/Porin/VDAC1 antibody for IHC for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
D. Gonzalez
Verified customer
Asked: 2017-08-31
Answer
As indicated on the product datasheet, PB9455 anti-VDAC/Porin/VDAC1 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-31
Question
My question regarding product PB9455, anti-VDAC/Porin/VDAC1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-08-04
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9455 anti-VDAC/Porin/VDAC1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-04
Question
We need to test anti-VDAC/Porin/VDAC1 antibody PB9455 on human brain for research purposes, then I may be interested in using anti-VDAC/Porin/VDAC1 antibody PB9455 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Evans
Verified customer
Asked: 2013-08-28
Answer
The products we sell, including anti-VDAC/Porin/VDAC1 antibody PB9455, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-08-28
Question
We have been able to see staining in rat cajal-retzius cell fetal brain cortex. Do you have any suggestions? Is anti-VDAC/Porin/VDAC1 antibody supposed to stain cajal-retzius cell fetal brain cortex positively?
M. Collins
Verified customer
Asked: 2013-06-04
Answer
From literature cajal-retzius cell fetal brain cortex does express VDAC1. From Uniprot.org, VDAC1 is expressed in biceps brachii, pituitary, thymus, lung skin, lymphocyte, skeletal muscle, b-cell, b-cell lymphoma, brain, cajal-retzius cell fetal brain cortex, cervix carcinoma, platelet, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have VDAC1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 7685903
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Skeletal muscle, Pubmed ID: 1657034
Boster Scientific Support
Answered: 2013-06-04
Question
See attached the WB image, lot number and protocol we used for brain using anti-VDAC/Porin/VDAC1 antibody PB9455. Please let me know if you require anything else.
S. Bhatt
Verified customer
Asked: 2013-06-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-06-03
