Anti-VDAC/Porin/VDAC1 Antibody Picoband™

Boster Bio Anti-VDAC/Porin/VDAC1 Antibody Picoband™ catalog # PB9455. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: PB9455
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-VDAC/Porin/VDAC1 Antibody Picoband™

See all VDAC1 products

SKU/Catalog Number

PB9455

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-VDAC/Porin/VDAC1 Antibody Picoband™ catalog # PB9455. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-VDAC/Porin/VDAC1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9455)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human VDAC/Porin (154-181aa QMNFETAKSRVTQSNFAVGYKTDEFQLH), different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9455 is reactive to VDAC1 in Human, Mouse, Rat

Applications

PB9455 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For VDAC1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P21796

Gene ID

7416

Gene Name

VDAC1

Full Name

Voltage-dependent anion-selective channel protein 1

Weight

30.773kDa

Superfamily

eukaryotic mitochondrial porin family

Alternative Names

hVDAC1; MGC111064; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Porin 31HL; Porin 31HM; PORIN; PORIN-31-HL; VDAC; VDAC-1; voltage-dependent anion channel 1; voltage-dependent anion-selective channel protein 1 VDAC1 PORIN, VDAC-1 voltage dependent anion channel 1 voltage-dependent anion-selective channel protein 1|outer mitochondrial membrane protein porin 1|plasmalemmal porin|porin 31HL|porin 31HM|sperm binding protein 1a

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on VDAC1, check out the VDAC1 Infographic

VDAC1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for VDAC1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9455 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Song H,Telugu BP,Thompson LP.Sexual dimorphism of mitochondrial function in the hypoxic guinea pig placenta.Biol Reprod.2019 Jan 1;100(1):208-216.doi:10.1093/biolre/ioy167.PMID:30085007;PMCID:PMC6335207.
Species: Pigs
PB9455 usage in article: APP:WB, SAMPLE:MITOCHONDRIA, DILUTION:1:2000

Wu L,Guo X,Wong SY,Lu P,Hartson SD,Medeiros DM,Wang W,Clarke SL,Lucas EA,Smith BJ,Chowanadisai W,Lin D. Deficiency of β-carotene oxygenase 2 induces mitochondrial fragmentation and activates the STING-IRF3 pathway in the mouse hypothalamus. J Nutr Biochem
Species: Mouse
PB9455 usage in article: APP:WB, SAMPLE:HYPOTHALAMIC PROTEINS AND MITOCHONDRIAL FRACTIONS, DILUTION:NA

Sexual dimorphism of mitochondrial function in the hypoxic guinea pig placenta Hong Song 1, Bhanu P Telugu 2 3, Loren P Thompson 1 Biol Reprod. 2019 Jan 1;100(1):208-216. doi: 10.1093/biolre/ioy167.

MicroRNA-27b Regulates Mitochondria Biogenesis in Myocytes

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16 Customer Q&As for Anti-VDAC/Porin/VDAC1 Antibody Picoband™

Question

Is a blocking peptide available for product anti-VDAC/Porin/VDAC1 antibody (PB9455)?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

We do provide the blocking peptide for product anti-VDAC/Porin/VDAC1 antibody (PB9455). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-14

Question

My question regards using your anti-VDAC/Porin/VDAC1 antibody for pyruvate metabolic process studies. Has this antibody been tested with western blotting on cardiac muscle tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

I appreciate your inquiry. This PB9455 anti-VDAC/Porin/VDAC1 antibody is validated on rat liver tissue, tissue lysate, mouse liver tissue, smmc whole cell lysate, cardiac muscle tissue, intestinal cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-02-10

Question

Will PB9455 anti-VDAC/Porin/VDAC1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

It shows on the product datasheet, PB9455 anti-VDAC/Porin/VDAC1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-31

Question

We are currently using anti-VDAC/Porin/VDAC1 antibody PB9455 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

The anti-VDAC/Porin/VDAC1 antibody (PB9455) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-06

Question

I see that the anti-VDAC/Porin/VDAC1 antibody PB9455 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-03

Question

Is this PB9455 anti-VDAC/Porin/VDAC1 antibody reactive to the isotypes of VDAC1?

Verified Customer

Verified customer

Asked: 2019-08-08

Answer

The immunogen of PB9455 anti-VDAC/Porin/VDAC1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human VDAC/Porin (154-181aa QMNFETAKSRVTQSNFAVGYKTDEFQLH), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-08

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-VDAC/Porin/VDAC1 antibody PB9455. Let me know if you need anything else.

A. Patel

Verified customer

Asked: 2019-06-10

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-10

Question

We have tried in the past anti-VDAC/Porin/VDAC1 antibody for WB on cervix carcinoma erythroleukemia a few months ago. I am using mouse, and We want to use the antibody for IHC next. I was wanting to use examining cervix carcinoma erythroleukemia as well as b-cell lymphoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

I have checked the website and datasheets of our anti-VDAC/Porin/VDAC1 antibody and it seems that PB9455 has been tested on mouse in both WB and IHC. Thus PB9455 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-06-03

Question

My team were content with the WB result of your anti-VDAC/Porin/VDAC1 antibody. However we have been able to see positive staining in skeletal muscle mitochondrion outer membrane using this antibody. Is that expected? Could you tell me where is VDAC1 supposed to be expressed?

A. Mangal

Verified customer

Asked: 2019-03-14

Answer

Based on literature, skeletal muscle does express VDAC1. Generally VDAC1 expresses in mitochondrion outer membrane. Regarding which tissues have VDAC1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 7685903
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Skeletal muscle, Pubmed ID: 1657034

Boster Scientific Support

Answered: 2019-03-14

Question

Would anti-VDAC/Porin/VDAC1 antibody PB9455 work for IHC with brain?

Verified Customer

Verified customer

Asked: 2019-02-11

Answer

According to the expression profile of brain, VDAC1 is highly expressed in brain. So, it is likely that anti-VDAC/Porin/VDAC1 antibody PB9455 will work for IHC with brain.

Boster Scientific Support

Answered: 2019-02-11

Question

Do you have a BSA free version of anti-VDAC/Porin/VDAC1 antibody PB9455 available?

Verified Customer

Verified customer

Asked: 2019-01-08

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-VDAC/Porin/VDAC1 antibody PB9455 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-01-08

Question

I was wanting to use your anti-VDAC/Porin/VDAC1 antibody for IHC for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?

D. Gonzalez

Verified customer

Asked: 2017-08-31

Answer

As indicated on the product datasheet, PB9455 anti-VDAC/Porin/VDAC1 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-08-31

Question

My question regarding product PB9455, anti-VDAC/Porin/VDAC1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-08-04

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9455 anti-VDAC/Porin/VDAC1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-08-04

Question

We need to test anti-VDAC/Porin/VDAC1 antibody PB9455 on human brain for research purposes, then I may be interested in using anti-VDAC/Porin/VDAC1 antibody PB9455 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

A. Evans

Verified customer

Asked: 2013-08-28

Answer

The products we sell, including anti-VDAC/Porin/VDAC1 antibody PB9455, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-08-28

Question

We have been able to see staining in rat cajal-retzius cell fetal brain cortex. Do you have any suggestions? Is anti-VDAC/Porin/VDAC1 antibody supposed to stain cajal-retzius cell fetal brain cortex positively?

M. Collins

Verified customer

Asked: 2013-06-04

Answer

From literature cajal-retzius cell fetal brain cortex does express VDAC1. From Uniprot.org, VDAC1 is expressed in biceps brachii, pituitary, thymus, lung skin, lymphocyte, skeletal muscle, b-cell, b-cell lymphoma, brain, cajal-retzius cell fetal brain cortex, cervix carcinoma, platelet, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have VDAC1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 7685903
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Skeletal muscle, Pubmed ID: 1657034

Boster Scientific Support

Answered: 2013-06-04

Question

See attached the WB image, lot number and protocol we used for brain using anti-VDAC/Porin/VDAC1 antibody PB9455. Please let me know if you require anything else.

S. Bhatt

Verified customer

Asked: 2013-06-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-06-03

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PB9455
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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