Rabbit IgG polyclonal antibody for Cytoglobin(CYGB) detection. Tested with WB in Human;Rat.
Anti-Cytoglobin/CYGB Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-Cytoglobin/CYGB Antibody
See all Cytoglobin primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for CYTOGLOBIN/CYGB detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. CYTOGLOBIN/CYGB information: Molecular Weight: 21405 MW; Subcellular Localization: Cytoplasm ; Tissue Specificity: Ubiquitously expressed. Highest expression in heart, stomach, bladder and small intestine.|
|Cite This Product||Anti-Cytoglobin/CYGB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1657)|
|Similar Products From
|Anti-Cytoglobin/CYGB Antibody may replace the following items: sc 245802|sc 245801.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Cytoglobin(50-68aa FVNFPSAKQYFSQFKHMED), identical to the related rat sequence, different from the related mouse sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For CYGB (Source: Uniprot.org, NCBI)
|NCBI Gene Id||114757|
|Species Of This Entry||Human|
|Alternative Names||Cytoglobin|cytoglobin; HGBHistoglobin; histoglobin; STAPHGb; Stellate cell activation-associated protein|
|Post Tranlational Modifications||Methylation; Phosphorylation; Reduction; Oxidation|
|Gene Location||17q25.1, on Chromasome 17, gene sequence: NC_000017.11 (76527356..76557692, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CYGB (Source: Uniprot.org, NCBI)
|Protein Function||May have a protective function during conditions of oxidative stress. May be involved in intracellular oxygen storage or transfer.|
|Tissue Specificity||Ubiquitously expressed. Highest expression in heart, stomach, bladder and small intestine.|
|Interacting Proteins||AKR7A2; DDI1|
|Related Diseases||Malignant Neoplasms; Neoplasms; Hypoxia; Fibrosis; Carcinoma; Anoxia; Lung Neoplasms; Malignant Neoplasm Of Lung; Malignant Paraganglionic Neoplasm; Liver Cirrhosis; Malignant Neoplasm Of Breast; Mouth Neoplasms; Non-small Cell Lung Carcinoma; Cell Transformation, Neoplastic; Malignant Squamous Cell Neoplasm; Kidney Diseases; Hepatic Fibrosis; Ovarian Neoplasm; Adenocarcinoma; Keratoderma, Palmoplantar, Diffuse|
|Related Pathways||Methylation; Transport; Localization; Cell Proliferation; Oxygen Transport; Cell Adhesion; Cell Cycle; Cell Death; Pathogenesis; Cell Activation; Cell Migration; Gene Silencing; Electron Transport; Electron Transport Chain; Dna Methylation; Cell Cycle Arrest; Translation; Mrna Stabilization; Cell Growth; Fibroblast Activation|
|Background||Cytoglobin(CYGB), also called HGB or STAP, is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress. The cytoglobin gene is mapped on 17q25.1. The CYGB gene contains 4 exons and spans about 9 kb. Cytoglobin has many elements common to vertebrate globins, including invariant histidine residues, and the amino acids that form the heme pocket share similarity with pentacoordinate myoglobin. In contrast to the high oxygen affinities displayed by most hexacoordinate hemoglobins, the characteristics of CYGB indicate that it can facilitate oxygen transport. Because the oxygen affinity of CYGB is more similar to myoglobin than to neuroglobin, and the oxy form of CYGB resists autooxidation, CYGB is proposed to represent a tissue oxygen reservoir by Sawai et al.|
|Scientific References||PMID: 11919282 by Burmester T., et al. Cytoglobin: a novel globin type ubiquitously expressed in vertebrate tissues.|
PMID: 12359339 by Asahina K., et al. Characterization of human stellate cell activation-associated protein and its expression in human liver.
Our Boster Quality Guarantee for Anti-Cytoglobin/CYGB Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti Cytoglobin (PA1657) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Small Intestine Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: Rat Kidney Tissue Lysate at 50ug
Lane 5: SGC Whole Cell Lysate at 40ug
Lane 6: COLO320 Whole Cell Lysate at 40ug
Lane 7: SMMC Whole Cell Lysate at 40ug
Lane 8: PANC Whole Cell Lysate at 40ug
Lane 9: HELA Whole Cell Lysate at 40ug
Predicted bind size: 21KD
Observed bind size: 21KD
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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