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- Table of Contents
KRT1 antibody (A01639) performs well in ELISA, Flow Cytometry, IF, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Flow Cytometry analysis of Jurkat cells using anti-Cytokeratin 1/KRT1 antibody (A01639).
Overlay histogram showing Jurkat cells stained with A01639 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytokeratin 1/KRT1 Antibody (A01639, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Jurkat cells stained with A01639 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytokeratin 1/KRT1 Antibody (A01639, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Cytokeratin 1/KRT1 Antibody Picoband®
KRT1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IF, IHC, WB |
Tested Applications
KRT1 antibody was positively detected by WB in:
human A375 whole cell, human Hela whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
KRT1 antibody was positively detected by IHC in:
human skin cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
KRT1 antibody was positively detected by IF in:
human skin cancer tissue
KRT1 antibody was positively detected by FC in:
Jurkat cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-Cytokeratin 1/KRT1 Antibody Picoband®
View all KRT1 Antibodies
View all KRT1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cytokeratin 1/KRT1 recombinant protein (Position: L215-Q431).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
66 kDa
Calculated Molecular Weight
66.0 kDa
Background of KRT1
Keratin 1 is a Type II intermediate filament (IFs) of the intracytoplasmatic cytoskeleton. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Cite This Product
Anti-Cytokeratin 1/KRT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01639)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Dog
88%
Moderate Homology
Rabbit
84%
Moderate Homology
Cow
83%
Moderate Homology
Sheep
82%
Moderate Homology
Mouse
81%
Moderate Homology
Zebrafish
59%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for KRT1 antibody
Immunohistochemistry (IHC) protocol for KRT1 antibody
Immunofluorescence (IF) protocol for KRT1 antibody
Flow Cytometry (FC) protocol for KRT1 antibody
Publications
Application:
Species:
LncRNA ANRIL inhibits TGF-β1-induced proliferation, migration, and fibrosis of Tenon’s capsule fibroblasts from glaucoma patients by reducing IL-22-RA1 stability and expression
Published: 2026-07-22
Journal: INTERNATIONAL OPHTHALMOLOGY
Application: IF
Impact Factor: 1.70
Species: Human
PMID: 42484723
Zeolitic imidazolate frameworks exert bioenergetic modulation via the cAMP/PKA/CREB signaling pathway to accelerate periodontal regeneration
Published: 2025-11-27
Journal: CHEMICAL ENGINEERING JOURNAL
Application: IF
Impact Factor: 13.20
Species: Human
Cyclic tensile forces enhance the angiogenic properties of HUVECs by promoting the activities of human periodontal ligament cells
Published: 2020-04-18
Journal: JOURNAL OF PERIODONTOLOGY
Application: IHC
Impact Factor: 3.74
Species: Human
PMID: 32304102
Improved biphasic calcium phosphate combined with periodontal ligament stem cells may serve as a promising method for periodontal regeneration
Published: 2018-12-15
Journal: American Journal of Translational Research
Application: IHC
Impact Factor: 3.06
Species: Dog
PMID: 30662648
Ventral hernia repair in rat using nanofibrous polylactic acid/polypropylene meshes
Published: 2018-07-12
Journal: Nanomedicine
Application: IF
Impact Factor: 5.01
Species: Rat
PMID: 29998792
High-temperature Requirement Protein A1 Regulates Odontoblastic Differentiation of Dental Pulp Cells via the Transforming Growth Factor Beta 1/Smad Signaling Pathway
Published: 2018-03-24
Journal: JOURNAL OF ENDODONTICS
Application: ICC
Impact Factor: 2.89
Species: Human
PMID: 29580722
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Product Promise
We promise Anti-Cytokeratin 1/KRT1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01639
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026