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- Table of Contents
KRT7 antibody (M02416-2) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Immunofluorescent analysis of HeLa cells, using Cytokeratin 7 Antibody.
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Anti-Cytokeratin 7 KRT7 Rabbit Monoclonal Antibody
KRT7 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
KRT7 antibody was positively detected by WB in:
human SIHA whole cell, human RT4 whole cell, human A549 whole cell, human Hela whole cell, rat lung tissue, rat kidney tissue, mouse lung tissue, mouse kidney tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
KRT7 antibody was positively detected by IHC in:
human uterus tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
KRT7 antibody was positively detected by ICC/IF in:
HeLa cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Product Information
Product Name
Anti-Cytokeratin 7 KRT7 Rabbit Monoclonal Antibody
View all KRT7 Antibodies
View all KRT7 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GEC-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytokeratin 7
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
40-50 kDa
Calculated Molecular Weight
51.4 kDa
Cite This Product
Anti-Cytokeratin 7 KRT7 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02416-2)
Protocols
Western Blot protocol for KRT7 antibody
Immunohistochemistry (IHC) protocol for KRT7 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for KRT7 antibody
Publications
Application:
Species:
Pretreatment with Inflammatory Factors Altered the Secretome of Human Amniotic Epithelial Cells
Published: 2024-06-19
Journal: Tissue Engineering Part C-Methods
Application: IF
Impact Factor: 2.70
Species: Human
PMID: 38756098
Heme oxygenase-1 gene modified human placental mesenchymal stem cells promote placental angiogenesis and spiral artery remodeling by improving the balance of angiogenic factors in vitro
Published: 2020-07-22
Journal: PLACENTA
Application: IF
Impact Factor: 3.18
Species: Human
PMID: 32758718
Immunomodulatory effect of human amniotic epithelial cells on restoration of ovarian function in mice with autoimmune ovarian disease
Published: 2019-07-09
Journal: ACTA BIOCHIMICA ET BIOPHYSICA SINICA
Application: IF
Impact Factor: 2.50
Species: Human
PMID: 31287492
Human amniotic epithelial cells inhibit growth of epithelial ovarian cancer cells via TGF‑β1-mediated cell cycle arrest
Published: 2017-09-14
Journal: INTERNATIONAL JOURNAL OF ONCOLOGY
Application: IF
Impact Factor: 3.08
Species: Human
PMID: 29048644
Review
Have you used Anti-Cytokeratin 7 KRT7 Rabbit Monoclonal Antibody?
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1 Review For Anti-Cytokeratin 7 KRT7 Rabbit Monoclonal Antibody
The Anti-Cytokeratin antibody (M02416-2) produced clear and specific target bands in WB analysis of untreated and drug-treated HTR8 cells, demonstrating reliable and consistent detection without non-specific signals.
| SKU | M02416-2 |
|---|---|
| Application | Western Blot |
| Sample | human HTR8 cells |
| Sample Processing Description | Cells were lysed with RIPA lysis buffer, mixed with β-mercaptoethanol, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE. |
| Other Reagents | blocking buffer |
| Primary Antibody | Cytokeratin 7 KRT7 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | 1 hour in room temperature |
| Detection | Substrate: ECL reagent, Imaging system:ChemiDoc MP |
| Results Summary | The figure shows WB results of Cytokeratin in untreated and drug-treated HTR8 cells, with clear and distinct bands at the expected position, demonstrating reliable and specific detection. |
Xiaoqin Yu， NIHWR
Verified customer
Submitted 2025-11-11
Product Promise
We promise Anti-Cytokeratin 7 KRT7 Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M02416-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026