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Product Info Summary
| SKU: | M02416-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cytokeratin 7 KRT7 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02416-2
BM4622 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 7 KRT7 Rabbit Monoclonal Antibody catalog # M02416-2. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 7 KRT7 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02416-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GEC-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytokeratin 7
Reactive Species
M02416-2 is reactive to KRT7 in Human, Mouse, Rat
Observed Molecular Weight
40-50 kDa
Calculated molecular weight
51.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02416-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: human SIHA whole cell, human RT4 whole cell, human A549 whole cell, human Hela whole cell, rat lung tissue, rat kidney tissue, mouse lung tissue, mouse kidney tissue
IHC: human uterus tissue
ICC/IF: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of Cytokeratin 7/KRT7 using anti-Cytokeratin 7/KRT7 antibody (M02416-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SIHA whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 7/KRT7 antigen affinity purified monoclonal antibody (M02416-2) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Cytokeratin 7/KRT7 at approximately 40-50 kDa. The expected band size for Cytokeratin 7/KRT7 is at 51 kDa.
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Immunohistochemical analysis of paraffin-embedded human uterus, using Cytokeratin 7 Antibody.
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Immunofluorescent analysis of HeLa cells, using Cytokeratin 7 Antibody.
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Western blot analysis of Cytokeratin 7/KRT7 using anti-Cytokeratin 7/KRT7 antibody (M02416-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1-4: control group-human HTR8 whole cell lysates,
Lane 5-9: model group-human HTR8 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 7/KRT7 antigen affinity purified polyclonal antibody (M02416-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate with ChemiDoc MP system. A specific band was detected for Cytokeratin 7/KRT7 at approximately 51 kDa. The expected band size for Cytokeratin 7/KRT7 is at 40-50 kDa.
Specific Publications For Anti-Cytokeratin 7 KRT7 Rabbit Monoclonal Antibody (M02416-2)
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Customer Reviews
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1 Reviews For Anti-Cytokeratin 7 KRT7 Rabbit Monoclonal Antibody
The Anti-Cytokeratin antibody (M02416-2) produced clear and specific target bands in WB analysis of untreated and drug-treated HTR8 cells, demonstrating reliable and consistent detection without non-specific signals.
Excellent
| SKU | M02416-2 |
|---|---|
| Application | Western Blot |
| Sample | human HTR8 cells |
| Sample Processing Description | Cells were lysed with RIPA lysis buffer, mixed with β-mercaptoethanol, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE. |
| Other Reagents | blocking buffer |
| Primary Antibody | Cytokeratin 7 KRT7 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | 1 hour in room temperature |
| Detection | Substrate: ECL reagent, Imaging system:ChemiDoc MP |
| Results Summary | The figure shows WB results of Cytokeratin in untreated and drug-treated HTR8 cells, with clear and distinct bands at the expected position, demonstrating reliable and specific detection. |
Xiaoqin Yu， NIHWR
Verified customer
Submitted 2025-11-11
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