Anti-MUC2 Antibody

IHC analysis of MUC2 using anti-MUC2 antibody (A01212).
MUC2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC2 Antibody (A01212) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

IHC analysis of MUC2 using anti-MUC2 antibody (A01212).
MUC2 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC2 Antibody (A01212) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

IL-22 is the key cytokine underlying the effects of the human infant Th17-conditioned media. (A) Experimental design for enteroid stimulation. (B) Representative immunofluorescent staining of Ki67 (red), E-cadherin (green), and DAPI (blue) in infant enteroids cultured in control media (left), control media supplemented with infant Th17-conditioned media (middle), or control media plus Th17-conditioned media plus neutralizing anti-IL-22 (right) (Scale bars, 50 μm). (C, E) Fluorescence intensity of Ki67 and MUC2 per enteroid relative to DAPI following exposure to Th17-conditioned media. (technical replicates n=5; N= 4 individuals, statistical difference calculated by one-way ANOVA). Enteroids exposed to infant Th17-conditioned media showed more goblet cell (MUC2) staining as compared to control, and Th17-conditioned media plus neutralizing IL-22 groups (Scale bar, 50 μm). (D) Infant enteroids stimulated by neonatal Th17-conditioned media display increased goblet cell numbers/size as indicated by increased MUC2 intensity per enteroid. MUC2 intensity was normalized to DAPI (technical replicates n=5; N= 4 individuals, statistical differences calculated by t-test **P < 0.01, *** P <0.001, **** P <0.0001) (Scale bar, 50 μm). Enteroid samples used N001, N002, N011 and N012.
Index in PubMed under a CC BY license. PMID: 40375988

IHC analysis of MUC2 using anti-MUC2 antibody (A01212).
MUC2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC2 Antibody (A01212) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

IF analysis of MUC2 using anti-MUC2 antibody (A01212).
MUC2 was detected in paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-MUC2 Antibody (A01212) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of MUC2 using anti-MUC2 antibody (A01212).
MUC2 was detected in paraffin-embedded section of human ileum tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-MUC2 Antibody (A01212) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of MUC2 using anti-MUC2 antibody (A01212).
MUC2 was detected in paraffin-embedded section of human colon organoid tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-MUC2 Antibody (A01212) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of MUC2 using anti-MUC2 antibody (A01212).
MUC2 was detected in paraffin-embedded section of mouse ileum tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-MUC2 Antibody (A01212) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of MUC2 using anti-MUC2 antibody (A01212).
MUC2 was detected in paraffin-embedded section of mouse ileum organoid tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-MUC2 Antibody (A01212) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of MUC2 using anti-MUC2 antibody (A01212).
MUC2 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-MUC2 Antibody (A01212) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.