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- Table of Contents
MUC2 antibody (A01212) performs well in IF, IHC and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 11 Publications
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IF analysis of MUC2 using anti-MUC2 antibody (A01212).
MUC2 was detected in paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-MUC2 Antibody (A01212) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MUC2 was detected in paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-MUC2 Antibody (A01212) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-MUC2 Antibody
MUC2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC |
Tested Applications
MUC2 antibody was positively detected by IHC in:
human colon cancer tissue, mouse colon tissue, rat colon tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
MUC2 antibody was positively detected by IF in:
human ileum tissue, human colon organoid tissue, mouse ileum tissue, mouse ileum organoid tissue, human intestine cancer tissue, mouse intestine tissue
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse, Rat
Immunofluorescence
5μg/ml
Human, Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
Human
Tested
Tested
Mouse
Tested
Tested
Rat
Tested
Recommend
Product Information
Product Name
Anti-MUC2 Antibody
View all MUC2 Antibodies
View all MUC2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MUC2, which shares 86.1% amino acid (aa) sequence identity with both mouse and rat MUC2.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Calculated Molecular Weight
540.3 kDa
Background of MUC2
Mucin 2, also known as MUC2, is a protein that in humans is encoded by the MUC2 gene. This gene encodes a member of the mucin protein family. It is mapped to 11p15.5. Mucin 2 is particularly prominent in the gut where it is secreted from goblet cells in the epithelial lining into the lumen of the large intestine. There, mucin 2, along with small amounts of related-mucin proteins, polymerizes into a gel of which 80% by weight is oligosaccharide side-chains that are added as post-translational modifications to the mucin proteins. This gel provides an insoluble mucous barrier that serves to protect the intestinal epithelium. The primary function of the MUC2 gene product is to provide a protective barrier between the epithelial surfaces and the gut lumen. There is decreased expression of MUC2 in colonic cancer and defective polymerization of secreted mucin in ulcerative colitis.
Cite This Product
Anti-MUC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01212)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
97%
High Homology
Cow
86%
Moderate Homology
Dog
86%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for MUC2 antibody
Immunofluorescence (IF) protocol for MUC2 antibody
Publications
Application:
Species:
Bifidobacterium bifidum Alleviate Intestinal Barrier Damage by Regulating the Intestinal Flora and Colonic Mucin O-Glycan Structural Patterns
Published: 2026-01-02
Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Application: IF
Impact Factor: 6.20
Species: Mouse
PMID: 41481539
Defining enteric bacterial pathogenesis using organoids: Citrobacter rodentium uses EspC, an atypical mucinolytic protease, to penetrate mouse colonic mucus
Published: 2025-05-05
Journal: Gut Microbes
Application: IF
Impact Factor: 11.00
Species: Mouse
PMID: 40323239
Crosstalk with infant-derived Th17 cells, as well as exposure to IL-22 promotes maturation of intestinal epithelial cells in an enteroid model
Published: 2025-05-01
Journal: Frontiers in Immunology
Application: IF
Impact Factor: 5.90
Species: Human
PMID: 40375988
Study repair function of mucin-2 on the tight junction protein of uterine epithelial cells under bacterial endotoxins
Published: 2024-11-08
Journal: TOXICON
Application: IF,IHC
Impact Factor: 2.60
Species: Mouse
PMID: 39522658
Altered Secretion, Constitution, and Functional Properties of the Gastrointestinal Mucus in a Rat Model of Sporadic Alzheimer’s Disease
Published: 2023-07-21
Journal: ACS Chemical Neuroscience
Application: Dot Blot
Impact Factor: 5.00
Species: Rat
PMID: 37477640
Central administration of human opiorphin alleviates dextran sodium sulfate-induced colitis in mice through activation of the endogenous opioid system
Published: 2022-09-13
Journal: Frontiers in Pharmacology
Application: IHC
Impact Factor: 5.99
Species: Mouse
PMID: 36176442
Maternal exposure to sodium ρ-perfluorous nonenoxybenzene sulfonate during pregnancy and lactation disrupts intestinal barrier and may cause obstacles to the nutrient transport and metabolism in F0 and F1 generations of mice
Published: 2021-07-01
Journal: SCIENCE OF THE TOTAL ENVIRONMENT
Application: IHC
Impact Factor: 7.96
Species: Mouse
PMID: 34323766
Bifidobacterium breve ATCC15700 pretreatment prevents alcoholic liver disease through modulating gut microbiota in mice exposed to chronic alcohol intake
Published: 2020-06-22
Journal: Journal of Functional Foods
Application: IHC
Impact Factor: 3.70
Species: Mouse
Subchronic exposure of environmentally relevant concentrations of F-53B in mice resulted in gut barrier dysfunction and colonic inflammation in a sex-independent manner
Published: 2019-07-09
Journal: ENVIRONMENTAL POLLUTION
Application: IHC
Impact Factor: 5.71
Species: Mouse
PMID: 31319243
Polysaccharide extracted from Enteromorpha ameliorates Cisplastin-induced small intestine injury in mice
Published: 2018-08-22
Journal: Journal of Functional Foods
Application: IHC
Impact Factor: 3.47
Species: Mouse
A Polysaccharide Isolated from Dictyophora indusiata Promotes Recovery from Antibiotic-Driven Intestinal Dysbiosis and Improves Gut Epithelial Barrier Function in a Mouse Model
Published: 2018-07-31
Journal: Nutrients
Application: IHC
Impact Factor: 4.20
Species: Mouse
PMID: 30065236
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Product Promise
We promise Anti-MUC2 Antibody performs excellently in relevant applications for IF, IHC. If it doesn't perform as promised, we will refund the product. Learn more
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A01212
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026