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- Table of Contents
MUC2 antibody (M01212-1) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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IHC analysis of MUC2 using anti-MUC2 antibody (M01212-1).
MUC2 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MUC2 Antibody (M01212-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
MUC2 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MUC2 Antibody (M01212-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-MUC2 Rabbit Monoclonal Antibody
MUC2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
MUC2 antibody was positively detected by WB in:
human Caco-2 whole cell, rat lung tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MUC2 antibody was positively detected by IHC in:
human colon tissue, human colon cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-MUC2 Rabbit Monoclonal Antibody
View all MUC2 Antibodies
View all MUC2 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAEO-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MUC2
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
110 kDa
Calculated Molecular Weight
540.3 kDa
Cite This Product
Anti-MUC2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01212-1)
Protocols
Western Blot protocol for MUC2 antibody
Immunohistochemistry (IHC) protocol for MUC2 antibody
Publications
Application:
Species:
Hederagenin Alleviates Ulcerative Colitis via Suppressing the Activation of EGFR/p38 MAPK Signaling
Published: 2026-05-13
Journal: JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
Application: WB
Impact Factor: 3.50
Species: Mouse,Rat
PMID: 42128880
Fecal microbiota transplantation from healthy piglets ameliorates intestinal inflammation in mice by modulating recipient metabolism
Published: 2026-01-12
Journal: BMC MICROBIOLOGY
Application: IHC
Impact Factor: 4.20
Species: Mouse
PMID: 41526816
TRPV4 agonist GSK1016790A increases mucus production and alleviates diabetic intestinal injury by activating the Ca2+/CAMKII/GSK3β pathway
Published: 2025-12-16
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 4.70
Species: Mouse
PMID: 41412260
Arabinoxylan from barley bran alleviates ulcerative colitis in mice through enhancement of mucosal barrier function and modulation of gut microbiota
Published: 2025-06-04
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB
Impact Factor: 8.50
Species: Mouse
PMID: 40480573
Dietary polyamines promote intestinal adaptation in an experimental model of short bowel syndrome
Published: 2024-02-26
Journal: Scientific Reports
Application: IHC
Impact Factor: 3.80
Species: Rat
PMID: 38409241
A mechanistic study of Anwei decoction intervention in a rat model of gastric intestinal metaplasia through the endoplasmic reticulum stress–autophagy pathway
Published: 2024-02-01
Journal: TISSUE & CELL
Application: WB
Impact Factor: 2.70
Species: Rat
PMID: 38330771
Evaluation of two laboratory model methods for diarrheal irritable bowel syndrome
Published: 2023-01-12
Journal: MOLECULAR MEDICINE
Application: IF
Impact Factor: 5.70
Species: Rat
PMID: 36635623
Liangxue Tongyu Prescription Alleviates Brain Damage in Acute Intracerebral Hemorrhage Rats by Regulating Intestinal Mucosal Barrier Function
Published: 2022-12-17
Journal: Evidence-based Complementary and Alternative Medicine
Application: WB
Impact Factor: 2.65
Species: Rat
PMID: 36573082
Prevention and Alleviation of Dextran Sulfate Sodium Salt-Induced Inflammatory Bowel Disease in Mice With Bacillus subtilis-Fermented Milk via Inhibition of the Inflammatory Responses and Regulation of the Intestinal Flora
Published: 2021-01-07
Journal: Frontiers in Microbiology
Application: WB
Impact Factor: 5.64
Species: Mouse
PMID: 33519783
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Product Promise
We promise Anti-MUC2 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01212-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026