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Product Info Summary
| SKU: | PA1314 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Decorin/DCN Antibody Picoband®
SKU/Catalog Number
PA1314
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Decorin/DCN Antibody catalog # PA1314. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Decorin/DCN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1314)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Decorin, identical to the related mouse sequence, and different from the related rat sequence by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1314 is reactive to DCN in Human, Mouse, Rat
Observed Molecular Weight
40-50 kDa, 80-140 kDa
Calculated molecular weight
39.7 kDa
Background of DCN
Decorin is a proteoglycan on average 90-140 kilodaltons (kD) in size. The human decorin gene spans more than 38 kb and contains 8 exons and very large introns, 2 of which are 5.4 and more than 13.2 kb. And the human decorin gene is mapped to 12q21.3. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS). Decorin is a small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. Decorin and biglycan are though to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. And it also may play a role in epithelial/mesenchymal interactions during organ development and shaping. Decorin has been shown to have anti-tumorigenic properties in an experimental murine tumor model and is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for the decorin gene. Mutations in the decorin gene are associated with congenital stromal corneal dystrophy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1314 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human MCF-7 whole cell, human Hela whole cell, human HepG2 whole cell, human SW620 whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse SP2/0 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Decorin using anti-Decorin antibody (PA1314).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human SW620 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse SP2/0 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Decorin antigen affinity purified polyclonal antibody (Catalog # PA1314) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for Decorin at approximately 40-50KD and 80-140KD. The expected band sizes for Decorin are at 40-50KD and 80-140KD.
Specific Publications For Anti-Decorin/DCN Antibody Picoband® (PA1314)
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Customer Q&As
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7 Customer Q&As for Anti-Decorin/DCN Antibody Picoband®
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Decorin/DCN antibody PA1314. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-20
Question
I have attached the WB image, lot number and protocol we used for lung using anti-Decorin/DCN antibody PA1314. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-06
Question
Is a blocking peptide available for product anti-Decorin/DCN antibody (PA1314)?
Verified Customer
Verified customer
Asked: 2019-01-16
Answer
We do provide the blocking peptide for product anti-Decorin/DCN antibody (PA1314). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-01-16
Question
I see that the anti-Decorin/DCN antibody PA1314 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-09-17
Question
Will anti-Decorin/DCN antibody PA1314 work on horse WB with decidua?
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
Our lab technicians have not validated anti-Decorin/DCN antibody PA1314 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Decorin/DCN antibody PA1314 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse decidua in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-29
Question
I was wanting to use your anti-Decorin/DCN antibody for WB for mouse lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse lung identification?
C. Krishna
Verified customer
Asked: 2016-04-21
Answer
It shows on the product datasheet, PA1314 anti-Decorin/DCN antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-04-21
Question
Is this PA1314 anti-Decorin/DCN antibody reactive to the isotypes of DCN?
T. Bhatt
Verified customer
Asked: 2013-03-26
Answer
The immunogen of PA1314 anti-Decorin/DCN antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Decorin(343-359aa TFRCVYVRSAIQLGNYK), identical to the related mouse sequence, and different from the related rat sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-03-26
