Anti-TGF beta2 TGFB2 Antibody

Boster Bio Anti-TGF beta2 TGFB2 Antibody catalog # A00892. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A00892
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC

Product Name

Anti-TGF beta2 TGFB2 Antibody

See all TGF-beta 2 products

SKU/Catalog Number

A00892

Size

100μl

Form

Liquid

Description

Boster Bio Anti-TGF beta2 TGFB2 Antibody catalog # A00892. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TGF beta2 TGFB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00892)

Host

Rabbit

Contents

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Isotype

IgG

Immunogen

Synthetic peptide from human protein at AA range: 61-110.

Reactive Species

A00892 is reactive to TGFB2 in Human, Mouse, Rat

Applications

A00892 is guaranteed for IHC Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

IHC-P, 1:50-300

Validation Images & Assay Conditions

Gene/Protein Information For TGFB2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P61812

Gene ID

7042

Gene Name

TGFB2

Full Name

Transforming growth factor beta-2 proprotein

Weight

47.748kDa

Superfamily

TGF-beta family

Alternative Names

BSC-1 cell growth inhibitor; cetermin; Glioblastoma-derived T-cell suppressor factor; G-TSF; MGC116892; polyergin; TGFB2; TGFbeta 2; TGF-beta 2; TGF-beta2; TGF-beta-2; transforming growth factor beta-2; transforming growth factor, beta 2 TGFB2 G-TSF, LDS4, TGF-beta2 transforming growth factor beta 2 transforming growth factor beta-2 proprotein|BSC-1 cell growth inhibitor|cetermin|glioblastoma-derived T-cell suppressor factor|polyergin|prepro-transforming growth factor beta-2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TGFB2, check out the TGFB2 Infographic

TGFB2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TGFB2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00892 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Tao YJ,Chen Q,Wang L,Yang X,Cun Q,Yang WY,Zhong H.Pirfenidone suppresses the abnormal activation of human Müller cells after platelet-derived growth factor-BB stimulation.Int J Ophthalmol.2019 Jul 18;12(7):1075-1082.doi:10.18240/ijo.2019.07.04.PMID:31341795;PMCID:PMC6629804.
Species: Human
A00892 usage in article: APP:WB, SAMPLE:MULLER CELL, DILUTION:NA

[HTML] Pirfenidone suppresses the abnormal activation of human Müller cells after platelet-derived growth factor-BB stimulation Yi-Jin Tao, et al. Int J Ophthalmol. 2019; 12(7): 1075–1082. Published online 2019 Jul 18. doi: 10.18240/ijo.2019.07.04

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14 Customer Q&As for Anti-TGF beta2 TGFB2 Antibody

Question

We are currently using anti-TGF beta2 antibody A00892 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

The anti-TGF beta2 antibody (A00892) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-29

Question

Is there a BSA free version of anti-TGF beta2 antibody A00892 available?

Verified Customer

Verified customer

Asked: 2020-04-27

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TGF beta2 antibody A00892 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-27

Question

I have attached the WB image, lot number and protocol we used for lung using anti-TGF beta2 antibody A00892. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-21

Question

We have seen staining in mouse lung. Any tips? Is anti-TGF beta2 antibody supposed to stain lung positively?

Verified Customer

Verified customer

Asked: 2020-03-04

Answer

From literature lung does express TGFB2. From Uniprot.org, TGFB2 is expressed in lung, thalamus, among other tissues. Regarding which tissues have TGFB2 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 1764261
Thalamus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-03-04

Question

My team were well pleased with the WB result of your anti-TGF beta2 antibody. However we have observed positive staining in thalamus latency-associated peptide: secreted, using this antibody. Is that expected? Could you tell me where is TGFB2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-02-18

Answer

From what I have seen in literature, thalamus does express TGFB2. Generally TGFB2 expresses in latency-associated peptide: secreted,, transforming growth factor beta-2: secreted. Regarding which tissues have TGFB2 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 1764261
Thalamus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-02-18

Question

Does anti-TGF beta2 antibody A00892 work for IHC with lung?

Verified Customer

Verified customer

Asked: 2020-02-17

Answer

According to the expression profile of lung, TGFB2 is highly expressed in lung. So, it is likely that anti-TGF beta2 antibody A00892 will work for IHC with lung.

Boster Scientific Support

Answered: 2020-02-17

Question

Can you help my question with product A00892, anti-TGF beta2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

E. Miller

Verified customer

Asked: 2020-02-11

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00892 anti-TGF beta2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-11

Question

Does A00892 anti-TGF beta2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-04

Answer

It shows on the product datasheet, A00892 anti-TGF beta2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-04

Question

I was wanting to use your anti-TGF beta2 antibody for IHC for mouse lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse lung identification?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

It shows on the product datasheet, A00892 anti-TGF beta2 antibody has been tested for IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-19

Question

Is this A00892 anti-TGF beta2 antibody reactive to the isotypes of TGFB2?

Verified Customer

Verified customer

Asked: 2018-11-07

Answer

The immunogen of A00892 anti-TGF beta2 antibody is Synthetic peptide from human protein at AA range: 61-110. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-11-07

Question

I see that the anti-TGF beta2 antibody A00892 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-10-26

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-10-26

Question

Is a blocking peptide available for product anti-TGF beta2 antibody (A00892)?

Verified Customer

Verified customer

Asked: 2018-01-02

Answer

We do provide the blocking peptide for product anti-TGF beta2 antibody (A00892). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-01-02

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-TGF beta2 antibody A00892. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-11-03

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-11-03

Question

My question regards to test anti-TGF beta2 antibody A00892 on mouse lung for research purposes, then I may be interested in using anti-TGF beta2 antibody A00892 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

K. Kulkarni

Verified customer

Asked: 2016-05-09

Answer

The products we sell, including anti-TGF beta2 antibody A00892, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-05-09

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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A00892
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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