Anti-Decorin/DCN Antibody Picoband®

Western blot analysis of Decorin using anti-Decorin antibody (PB9174).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human SW620 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse HEPA1-6 whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Decorin antigen affinity purified polyclonal antibody (PB9174) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Decorin at approximately 70 kDa. The expected band size for Decorin is at 40 kDa.

IHC analysis of Decorin using anti-Decorin antibody (PB9174).
Decorin was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Decorin Antibody (PB9174) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

The AKT inhibitor MK2206 abolished the effects induced by overexpression of decorin. ( A ) The tube formation test; the photographs were taken at a magnification of 100×. ( B ) The cell wound healing test; the photographs were taken at a magnification of 100×. ( C ) The apoptosis assay. ( D ) CCK8 assessment. ( E , F ) The expression of VEGF, Bcl2, and Bax and the phosphorylation of AKT and AP 1. All data are presented as the mean ± SEM. *p < 0.05; **p < 0.01. # p < 0.05, ## p < 0.01, compared to Con. & p < 0.05, && p < 0.01, compared to HG + GFP (HG). $ p < 0.05, $$ p < 0.01, compared to HG + DCN.
Index in PubMed under a CC BY license. PMID: 28290552

The IGF1R antibody (αIGF1R) blocked the effects induced by overexpression of decorin. ( A , B ) The tube formation test; the photographs were taken at a magnification of 100×. ( C , D ) The cell wound healing test; the photographs were taken at a magnification of 100×. ( E , F ) The apoptosis assay. ( G ) CCK8 assessment. ( H , I ) The expression of VEGF, Bcl2, and Bax and the phosphorylation of AKT and AP 1. All data are presented as the mean ± SEM. *p < 0.05; **p < 0.01. # p < 0.05, ## p < 0.01, compared to Con. & p < 0.05, && p < 0.01, compared to HG + GFP. $ p < 0.05, $$ p < 0.01, compared to HG + DCN.
Index in PubMed under a CC BY license. PMID: 28290552

Overexpression of decorin activated the IGF1R-AKT-AP-1 pathway. ( A , B ) The phosphorylation of AKT was decreased by HG treatment in a time-dependent manner. ( C – E ) The expression of IGF1R, Bcl2, and Bax and the phosphorylation of AKT. ( F , G ) The phosphorylation of AP-1. All data are presented as the mean ± SEM. *p < 0.05; **p < 0.01. # p < 0.05, compared to Con. & p < 0.05, && p < 0.01, compared to HG + GFP.
Index in PubMed under a CC BY license. PMID: 28290552

Overexpression of decorin ameliorated the angiogenesis impaired by high glucose (HG). ( A – C ) The expression of decorin and VEGF. ( D , E ) The tube formation test; the photographs were taken at a magnification of 100×. ( F , G ) The cell wound healing test; the photographs were taken in a magnification of 100×. ( H , I ) The apoptosis rate analyzed by an Annexin V–FITC kit through flow cytometry. ( J ) The proliferative ability assessed with a CCK8 assay. All data are presented as the mean ± SEM. *p < 0.05; **p < 0.01.
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Overexpression of decorin increased angiogenesis in the diabetic hearts. ( A – C ) The expression of decorin and VEGF. ( D ) The VEGF concentration in the plasma. ( E ) The capillary density using immunochemistry with a CD31 antibody. The photographs were taken at a magnification of 100× and zoomed at a magnification of 200×. The arrows show the capillary stained with the CD31 antibody. ( F ) The number of vessels counted from the immunochemistry staining. All data are presented as the mean ± SEM. *p < 0.05; **p < 0.01.
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Overexpression of decorin improved cardiac function in diabetic cardiomyopathy. ( A ) The oral glucose tolerance test (OGTT). ( B ) The left ventricular ejection fraction (EF) evaluated by echocardiography. ( C ) The fraction shortening (FS) evaluated by echocardiography. ( D , E ) The left ventricular wall thickness (including the interventricular septum (IVS) and the posterior wall (PW)) and the internal dimension of the left ventricle (LVID). ( F – H ) The hemodynamic function was evaluated by the cardiac catheter system, including the left ventricular end-diastolic pressure (LVEDP), as well as the Dp/dt maximum and minimum. All data are presented as the mean ± SEM. *p < 0.05; **p < 0.01.
Index in PubMed under a CC BY license. PMID: 28290552