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Product Info Summary
| SKU: | M02090 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-Biglycan Monoclonal Antibody
SKU/Catalog Number
M02090
BM5625 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Biglycan Monoclonal Antibody catalog # M02090. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Biglycan Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02090)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEIB-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Biglycan May be involved in collagen fiber assembly.
Reactive Species
M02090 is reactive to BGN in Human, Mouse, Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
41.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02090 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:50
FC 1:100
Positive Control
WB: C2C12 cell
Validation Images & Assay Conditions
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Western blot analysis of Biglycan expression in C2C12 cell lysate.
Specific Publications For Anti-Biglycan Monoclonal Antibody (M02090)
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Customer Q&As
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8 Customer Q&As for Anti-Biglycan Monoclonal Antibody
Question
I am interested in to test anti-Biglycan Monoclonal antibody M02090 on rat brain for research purposes, then I may be interested in using anti-Biglycan Monoclonal antibody M02090 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
The products we sell, including anti-Biglycan Monoclonal antibody M02090, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-16
Question
What sample was tested in WB and IHC applications with M02090?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of Biglycan expression in C2C12 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-Biglycan Monoclonal antibody M02090 for human tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
The anti-Biglycan Monoclonal antibody (M02090) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-22
Question
I see that the anti-Biglycan Monoclonal antibody M02090 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-17
Question
I was wanting to use your anti-Biglycan Monoclonal antibody for IP for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
As indicated on the product datasheet, M02090 anti-Biglycan Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-09
Question
Will anti-Biglycan Monoclonal antibody M02090 work on canine WB with bone?
L. Mangal
Verified customer
Asked: 2017-04-10
Answer
Our lab technicians have not validated anti-Biglycan Monoclonal antibody M02090 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Biglycan Monoclonal antibody M02090 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine bone in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-04-10
Question
Will anti-Biglycan Monoclonal antibody M02090 work for IP with brain?
O. Dhar
Verified customer
Asked: 2015-05-20
Answer
According to the expression profile of brain, BGN is highly expressed in brain. So, it is likely that anti-Biglycan Monoclonal antibody M02090 will work for IP with brain.
Boster Scientific Support
Answered: 2015-05-20
Question
Is a blocking peptide available for product anti-Biglycan Monoclonal antibody (M02090)?
P. Singh
Verified customer
Asked: 2015-01-21
Answer
We do provide the blocking peptide for product anti-Biglycan Monoclonal antibody (M02090). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-01-21
