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Product Info Summary
| SKU: | M16262 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-DHRS4L2 Mouse Monoclonal Antibody [Clone ID: OTI4A10]
SKU/Catalog Number
M16262
Size
100 µl
Description
Boster Bio DHRS4L2 mouse monoclonal antibody,clone OTI4A10. Catalog# M16262. Tested in IHC, WB. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-DHRS4L2 Mouse Monoclonal Antibody [Clone ID: OTI4A10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M16262)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI4A10
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human DHRS4L2 (NP_932349) produced in E.coli.
Reactive Species
M16262 is reactive to DHRS4L2 in Human, Mouse
Calculated molecular weight
24.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M16262 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500~2000
IHC 1:250
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DHRS4L2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHRS4L2 (1:2000).
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Western blot analysis of extracts (35ug) from Hepg2 cell line and mouse kidney tissue lysate by using anti-DHRS4L2 monoclonal antibody (1:500).
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Immunohistochemical staining of paraffin-embedded Human placenta tissue within the normal limits using anti-DHRS4L2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-DHRS4L2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
Specific Publications For Anti-DHRS4L2 Mouse Monoclonal Antibody [Clone ID: OTI4A10] (M16262)
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