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Product Info Summary
| SKU: | A02320-1 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD151 Antibody Picoband®
SKU/Catalog Number
A02320-1
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD151 Antibody Picoband® catalog # A02320-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD151 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02320-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human CD151 recombinant protein (Position: A113-A175). Human CD151 shares 88.7% amino acid (aa) sequence identity with both mouse and rat CD151.
Cross-reactivity
No cross reactivity with other proteins
Reactive Species
A02320-1 is reactive to CD151 in Human, Mouse, Rat
Observed Molecular Weight
28 kDa
Calculated molecular weight
28.3 kDa
Background of CD151
CD151 molecule (Raph blood group), also known as CD151 (Cluster of Differentiation 151), is a human gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells. Multiple alternatively spliced transcript variants that encode the same protein have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02320-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Positive Control
WB: human A549 whole cell, human HepG2 whole cell, rat PC-12 whole cell, rat RH35 whole cell, mouse RAW264.7 whole cell
Validation Images & Assay Conditions
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Western blot analysis of CD151 using anti-CD151 antibody (A02320-1).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: rat RH35 whole cell lysates,
Lane 5: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD151 antigen affinity purified polyclonal antibody (A02320-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD151 at approximately 28 kDa. The expected band size for CD151 is at 28 kDa.
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IHC analysis of CD151 using anti-CD151 antibody (A02320-1).
CD151 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD151 Antibody (A02320-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-CD151 Antibody Picoband® (A02320-1)
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Customer Q&As
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6 Customer Q&As for Anti-CD151 Antibody Picoband®
Question
Would anti-CD151 antibody A02320-1 work on feline WB with liver?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
Our lab technicians have not tested anti-CD151 antibody A02320-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-CD151 antibody A02320-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-30
Question
Will anti-CD151 antibody A02320-1 work for WB with descending thoracic aorta?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
According to the expression profile of descending thoracic aorta, CD151 is highly expressed in descending thoracic aorta. So, it is likely that anti-CD151 antibody A02320-1 will work for WB with descending thoracic aorta.
Boster Scientific Support
Answered: 2020-01-03
Question
We are currently using anti-CD151 antibody A02320-1 for mouse tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
The anti-CD151 antibody (A02320-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-15
Question
Is there a BSA free version of anti-CD151 antibody A02320-1 available?
Verified Customer
Verified customer
Asked: 2019-01-25
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD151 antibody A02320-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-25
Question
I was wanting to use your anti-CD151 antibody for WB for human descending thoracic aorta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human descending thoracic aorta identification?
C. Bhatt
Verified customer
Asked: 2016-01-29
Answer
It shows on the product datasheet, A02320-1 anti-CD151 antibody has been tested for Flow Cytometry, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human descending thoracic aorta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-01-29
Question
I see that the anti-CD151 antibody A02320-1 works with WB, what is the protocol used to produce the result images on the product page?
H. Singh
Verified customer
Asked: 2013-01-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-01-07
