Anti-Lipoamide Dehydrogenase/DLD Antibody
Rabbit IgG polyclonal antibody for Dihydrolipoyl dehydrogenase, mitochondrial(DLD) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Anti-Lipoamide Dehydrogenase/DLD Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Applications:||IHC-P, IHC-F, ICC, WB|
|Product Name||Anti-Lipoamide Dehydrogenase/DLD Antibody
See all Dihydrolipoamide Dehydrogenase/DLD primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for dihydrolipoamide dehydrogenase/DLD detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. dihydrolipoamide dehydrogenase/DLD information: Molecular Weight: 54177 MW; Subcellular Localization: Mitochondrion matrix.|
|Cite This Product||Anti-Lipoamide Dehydrogenase/DLD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1463)|
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|Anti-Lipoamide Dehydrogenase/DLD Antibody may replace the following items: sc 55932|sc 376890|sc 271569|sc 135027|sc 365977.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||IHC-P, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human DLD(492-509aa EAFREANLAASFGKSINF), different from the related mouse and rat sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For DLD (Source: Uniprot.org, NCBI)
|NCBI Gene Id||1738|
|Species Of This Entry||Human|
|Protein Name||Dihydrolipoyl dehydrogenase, mitochondrial|
|Superfamily||class-I pyridine nucleotide-disulfide oxidoreductase family|
|Alternative Names||Dihydrolipoamide Dehydrogenase/DLD|2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex); branched chain keto acid dehydrogenase complex; Diaphorase; dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex; Dihydrolipoamide Dehydrogenase; dihydrolipoamide dehydrogenasemitochondrial; DLD; DLDD; DLDH; E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex; E3; EC 1.8.1; EC 18.104.22.168; GCSL; Glycine cleavage system L protein; glycine cleavage system protein L; LAD; Lipoamide Dehydrogenase; lipoamide reductase; lipoyl dehydrogenase; PHE3|
|Post Tranlational Modifications||Oxidation; Cleavage; Phosphorylation; Methylation; Ubiquitination; Demethylation; Nitration; Decarboxylation|
|Subcellular Localization||Mitochondrion matrix. Nucleus. Cell projection, cilium, flagellum. Cytoplasmic vesicle, secretory vesicle, acrosome. Mainly localizes in the mitochondrion. A small fraction localizes to the nucleus, where the 2-oxoglutarate dehydrogenase complex is required for histone succinylation.|
|Gene Location||7q31.1, on Chromasome 7, gene sequence: NC_000007.14 (107891107..107921198)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For DLD (Source: Uniprot.org, NCBI)
|Protein Function||Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex) (PubMed:15712224, PubMed:16442803, PubMed:16770810, PubMed:17404228, PubMed:20160912, PubMed:20385101). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion (PubMed:29211711). A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (PubMed:29211711). In monomeric form may have additional moonlighting function as serine protease (PubMed:17404228). Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction (By similarity).|
|Interacting Proteins||PDHX; PRDX6; YWHAE|
|Related Diseases||Language Development Disorders; Language Disorders; Autistic Disorder; Lung Diseases; Malignant Tumor Of Colon; Neoplasms; Malignant Neoplasms; Hypertensive Disease; Carcinoma; Malnutrition; Acidosis; Colonic Neoplasms; Pulmonary Fibrosis; Developmental Disabilities; Alzheimer's Disease; Lung Diseases, Interstitial; Fibrosis; Diabetes Mellitus|
|Related Pathways||Pathogenesis; Localization; Transport; Cell Growth; Fermentation; Angiogenesis; Regeneration; Acrosome Reaction; Electron Transport; Hypersensitivity; Neurogenesis; Oxidative Phosphorylation; Methylation; Cytokine Production; Cell Death; Sperm Capacitation; Cell Adhesion; Osteoblast Differentiation; Sulfate Reduction; Chondrocyte Hypertrophy|
|Background||DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex(BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.|
|Scientific References||PMID: 3693355 by Otulakowski G., et al. Isolation and sequence determination of cDNA clones for porcine and human lipoamide dehydrogenase. Homology to other disulfide oxidoreductases.|
PMID: 3278312 by Pons G., et al. Cloning and cDNA sequence of the dihydrolipoamide dehydrogenase component human alpha-ketoacid dehydrogenase complexes.
Our Boster Quality Guarantee for Anti-Lipoamide Dehydrogenase/DLD Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Ovary Tissue Lysate
Lane 4: Rat Testis Tissue Lysate
Lane 5: SMMC Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 8: JURKAT Cell Lysate
IHC(P): Human Mammary Cancer Tissue
ICC: MCF-7 Cell
DLD was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti- DLD Antibody (PA1463) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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