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- Table of Contents
Tnfrsf25 antibody (A03227-3) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of NRK cells using anti-DR3/Tnfrsf25 antibody (A03227-3).
Overlay histogram showing NRK cells stained with A03227-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DR3/Tnfrsf25 Antibody (A03227-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing NRK cells stained with A03227-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DR3/Tnfrsf25 Antibody (A03227-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Designing a Western blot for TNFRSF25? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the TNFRSF25 Western Blot GuideBundle Save
Anti-DR3/Tnfrsf25 Antibody Picoband®
Tnfrsf25 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
Tnfrsf25 antibody was positively detected by WB in:
mouse SP2/0 whole cell, mouse RAW2647 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Tnfrsf25 antibody was positively detected by FC in:
NRK cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Rat
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-DR3/Tnfrsf25 Antibody Picoband®
View all TNFRSF25 Antibodies
View all TNFRSF25 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat DR3/Tnfrsf25 recombinant protein (Position: E43-E393).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Background of Tnfrsf25
TNFRSF25 (Tumor Necrosis Factor Receptor Superfamily Member 25), also known as LARD, APO3, DR3 or TNFR25, is a protein that in humans is encoded by the TNFRSF25 gene. Members of the mammalian tumor necrosis factor receptor (TNFR) family are cell-surface proteins that interact with a corresponding TNF-related ligand family. By fluorescence in situ hybridization, Marsters et al. (1996) mapped the Apo3 gene to 1p36.3. Marsters et al. (1996) showed that ectopic expression of Apo3 in mammalian cells triggered apoptosis and activated the transcription factor NF-kappa-B. They suggested that, like TNFR1, Apo3 may regulate distinct signaling pathways in different cellular contexts.
Cite This Product
Anti-DR3/Tnfrsf25 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03227-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Rat
100%
High Homology
Mouse
88%
Moderate Homology
Rabbit
69%
Low homology
Human
67%
Low homology
Dog
64%
Low homology
Monkey
64%
Low homology
Sheep
63%
Low homology
Cow
63%
Low homology
Zebrafish
27%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Tnfrsf25 antibody
Flow Cytometry (FC) protocol for Tnfrsf25 antibody
Publications
Application:
Species:
Therapeutic effect of daphnetin on the autoimmune arthritis through demethylation of proapoptotic genes in synovial cells
Published: 2014-10-14
Journal: Journal of Translational Medicine
Application: FC
Impact Factor: 3.99
Species: Rat
PMID: 25311560
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Product Promise
We promise Anti-DR3/Tnfrsf25 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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A03227-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 17, 2026