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Product Info Summary
| SKU: | M00556-4 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DRP1/DNM1L (Phospho-S616) Antibody (Monoclonal, 34D03)
SKU/Catalog Number
M00556-4
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-DRP1/DNM1L (Phospho-S616) Antibody (Monoclonal, 34D03) catalog # M00556-4. Tested in WB, IHC, IF, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DRP1/DNM1L (Phospho-S616) Antibody (Monoclonal, 34D03) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00556-4)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
34D03
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser616 of human DRP1.
Reactive Species
M00556-4 is reactive to DNM1L in Human, Mouse, Rat
Observed Molecular Weight
75 kDa
Calculated molecular weight
81.9 kDa
Background of DNM1L
Dynamin-1-like protein is a GTPase that regulates mitochondrial fission. In humans, dynamin-1-like protein, which is typically referred to as dynamin-related protein 1 (Drp1), is encoded by the DNM1L gene. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00556-4 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-200
Immunofluorescence, 1:50-200
Immunocytochemistry/Immunofluorescence, 1:50-200
Flow Cytometry (Fixed), 1:50-200
Positive Control
WB: human Hela whole cell, human A549 whole cell, human HepG2 whole cell, human Caco-2 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
IHC: mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of P-DRP1 using anti-P-DRP1 antibody (M00556-4).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P-DRP1 antigen affinity purified monoclonal antibody (M00556-4) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P-DRP1 at approximately 75 kDa. The expected band size for P-DRP1 is at 82 kDa.
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IHC analysis of P-DRP1 using anti-P-DRP1 antibody (M00556-4).
P-DRP1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-P-DRP1 Antibody (M00556-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-DRP1/DNM1L (Phospho-S616) Antibody (Monoclonal, 34D03) (M00556-4)
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