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- Table of Contents
EGFR antibody (M00023-2) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Anti-EGFR (ErbB 1) Monoclonal Antibody
EGFR antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
EGFR antibody was positively detected by WB in:
human Hela whole cell, human A431 whole cell, human A549 whole cell, human U-87MG whole cell, rat liver tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
EGFR antibody was positively detected by IHC in:
Rat cerebral cortex tissue, Rat stomach tissue, Human thyroid cancer tissue, Human glioblastoma tissue, Mouse intestine tissue, human stomach cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-EGFR (ErbB 1) Monoclonal Antibody
View all EGFR Antibodies
View all EGFR Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEF-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human EGFR (ErbB 1)
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
175 kDa
Calculated Molecular Weight
134.3 kDa
Cite This Product
Anti-EGFR (ErbB 1) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00023-2)
Protocols
Western Blot protocol for EGFR antibody
Immunohistochemistry (IHC) protocol for EGFR antibody
Publications
Application:
Species:
Fabrication of sac-like hydrogel membranes for replicating curved tissue barriers on chips
Published: 2023-11-20
Journal: LAB ON A CHIP
Impact Factor: 6.10
PMID: 38018218
GO nanosheets inhibit the proliferation of hPDLCs by covering the membrane to block the EGFR-AKT signaling pathway
Published: 2023-10-25
Journal: Colloid and Interface Science Communications
Application: WB
Impact Factor: 4.50
Species: Mouse
Loss of EGFR contributes to high-fat diet-induced nonalcoholic fatty liver disease
Published: 2023-05-12
Journal: FEBS LETTERS
Application: WB
Impact Factor: 3.50
Species: Mouse
PMID: 37171232
Explore the mechanism and substance basis of Mahuang FuziXixin Decoction for the treatment of lung cancer based on network pharmacology and molecular docking
Published: 2022-11-10
Journal: COMPUTERS IN BIOLOGY AND MEDICINE
Application: WB
Impact Factor: 6.70
Species: Human
PMID: 36399857
Glucocorticoid mediates prenatal caffeine exposure-induced endochondral ossification retardation and its molecular mechanism in female fetal rats
Published: 2017-10-26
Journal: Cell Death & Disease
Application: WB
Impact Factor: 5.97
Species: Rat
PMID: 29072695
A water-soluble polysaccharide from the roots of Polygala tenuifolia suppresses ovarian tumor growth and angiogenesis in vivo
Published: 2017-09-15
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: IHC
Impact Factor: 3.67
Species: Mouse
PMID: 28923567
Review
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1 Review For Anti-EGFR (ErbB 1) Monoclonal Antibody
WB analysis using Anti-EGFR antibody (M00023-2) in mouse hippocampal tissue showed a clear band at the expected molecular weight with low background, demonstrating good specificity and reliable performance.
| SKU | M00023-2 |
|---|---|
| Application | Western Blot |
| Sample | mouse hippocampal tissue |
| Sample Processing Description | The left hippocampus was dissected from normal mouse brains, and total protein was extracted. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | EGFR (ErbB 1) Monoclonal Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated goat anti-rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in RT |
| Detection | Substrate: ECL substrate; Image system: ChemiDoc MP |
| Results Summary | EGFR protein is a key molecular switch located on the cell membrane that receives extracellular signals and drives cell proliferation and differentiation through kinase activity. It serves as a central regulator of organ development, tissue homeostasis, and regenerative repair. In this study, hippocampal tissues from two normal mouse brains were used to evaluate the performance of the BDNF antibody. The results showed a clear and correctly positioned target band, indicating that the antibody is functional and works properly. |
Yajun Qiao, Qinghai University
Verified customer
Submitted 2026-05-08
Product Promise
We promise Anti-EGFR (ErbB 1) Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00023-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026