Anti-EGFR (ErbB 1) Monoclonal Antibody

Effects of PCE on the expression changes of Mig-6 and EGFR in fetal long-bone hypertrophic chondrocytes. ( a ) ISH of Mig-6 in hypertrophic chondrocytes. ( b ) Immunostaining of Mig-6 in hypertrophic chondrocytes. ( c ) Immunostaining of EGFR in hypertrophic chondrocytes. ( d ) Serum corticosterone (CORT) concentration of fetal rats (ng/ml). ( e ) Quantification of Mig-6 ISH (fluorescence intensity). ( f ) Quantification of Mig-6 immunostaining (optical density). ( g ) Quantification of EGFR immunostaining (optical density). n =5 per group obtained from different litters. Three random fields/section for quantitative. Data are shown as the mean±S.D. * P <0.05, ** P <0.01 versus control (ANOVA)
Index in PubMed under a CC BY license. PMID: 29072695

Effects of corticosterone (250–1250 nM) with/without siRNA (Mig-6, EGFR) for 48 h on rats primary chondrocytes terminal differentiation and apoptosis. ( a and b ) mRNA expression of mitogen-inducible gene 6 (Mig-6) and EGFR after corticosterone and Mig-6 siRNA treatment, ( c ) Protein expression of Mig-6, EGFR, phosphorylated EGFR (P-EGFR), c-Jun N-terminal kinase (JNK) and Phosphorylated JNK (P-JNK) detected by western blotting after corticosterone and Mig-6 siRNA treatment. ( d – h ) Quantification of Mig-6, EGFR, P-EGFR, JNK and P-JNK (relative grayscale). ( i – k ) mRNA expression of runt-related transcription factor 2 (Runx2), collagen type X (Col-X) and matrix metalloproteinases-13 (MMP-13) after corticosterone and Mig-6 siRNA treatment. ( l and m ) Apoptotic analysis detected by Annexin V/PI after corticosterone and Mig-6 siRNA treatment. ( n ) Protein expression of EGFR, JNK and P-JNK detected by western blotting after EGFR siRNA treatment. ( o ) Quantification of Mig-6, EGFR, P-EGFR, JNK and P-JNK (Relative grayscale). ( p ) mRNA expression of Runx2, Col-X and MMP-13 after EGFR siRNA treatment. ( q ) Apoptotic analysis detected by Annexin V/PI after EGFR siRNA treatment. Data are shown as the mean±S.D. of results from three experiments. * P <0.05, ** P <0.01 versus control; # P <0.05, ## P <0.01 versus CORT treatment group. (A-M, ANOVA; O-R, t test)
Index in PubMed under a CC BY license. PMID: 29072695

The proposed schematic model of the present study. Col-X, collagen type X; EGFR, epidermal growth factor receptor; JNK, c-Jun N-terminal kinase; Mig-6, mitogen-inducible gene 6; MMP-13, matrix metallopeptidase 13; Runx2, runt-related transcription factor 2
Index in PubMed under a CC BY license. PMID: 29072695

Western blot analysis of EGFR using anti-EGFR antibody (M00023-2).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549- WT whole cell lysates,
Lane 2: human A549-EGFR KO whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR antigen affinity purified monoclonal antibody (M00023-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EGFR at approximately 175 kDa. The expected band size for EGFR is at 134 kDa.

Western blot analysis of EGFR using anti-EGFR antibody (M00023-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human U-87MG whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR antigen affinity purified monoclonal antibody (Catalog # M00023-2) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR at approximately 175 kDa. The expected band size for EGFR is at 134 kDa.

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using the Antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded Mouse intestine, using the Antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded human stomach cancer, using EGFR (ErbB 1) Antibody.

Immunofluorescent analysis using the Antibody at 1:50 dilution.

Immunofluorescent analysis using the Antibody at 1:50 dilution.

Immunofluorescent analysis using the Antibody at 1:150 dilution.

Immunofluorescent analysis using the Antibody at 1:500 dilution.

Western blot analysis of EGFR using anti-EGFR antibody (M00023-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1-2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR antigen affinity purified monoclonal antibody (Catalog # M00023-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for EGFR at approximately 175 kDa. The expected band size for EGFR is at 134 kDa.