Human FLRT3 ELISA Kit PicoKine™

Human FLRT3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FLRT3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK1974
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Human FLRT3 ELISA Kit PicoKine™

See all FLRT3 products

SKU/Catalog Number

EK1974

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human FLRT3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FLRT3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human FLRT3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1974)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: K29-S649

Sensitivity

<50 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1974 is reactive to FLRT3 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1974 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1974-CAP Anti-Human FLRT3 Pre-coated 96-well strip microplate 1
EK1974-ST Human FLRT3 Standard 2 vials, 10 ng/tube
EK1974-DA Human FLRT3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0400.1320.2120.3350.7191.1611.8682.462

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect FLRT3, Dilution ratio of 1:1, concentration in serum is around 300pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human FLRT3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2061152431822812364423
Standard deviation12.1549.54220.2215.0571.69203.46
CV (%)5.9%4.3%5.1%6.6%5.8%4.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of FLRT3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12062282122032129.654.5%
Sample 21152110411471147113719.441.7%
Sample 343183995441336354090305.107.4%
*number of samples for each test n=16.

Gene/Protein Information For FLRT3 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9NZU0

Gene ID

23767

Gene Name

FLRT3

Full Name

Leucine-rich repeat transmembrane protein FLRT3

Weight

73.004kDa

Alternative Names

fibronectin leucine rich transmembrane protein 3; Fibronectin-like domain-containing leucine-rich transmembrane protein 3; FLRT3; HH21; KIAA1469; leucine-rich repeat transmembrane protein FLRT3 FLRT3 HH21 fibronectin leucine rich transmembrane protein 3 leucine-rich repeat transmembrane protein FLRT3|fibronectin-like domain-containing leucine-rich transmembrane protein 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FLRT3, check out the FLRT3 Infographic

FLRT3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FLRT3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1974

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5 Customer Q&As for Human FLRT3 ELISA Kit PicoKine™

Question

Q: can I use citrate plasma as samples in Human FLRT3 Picokine® ELISA Kit (Catalog # EK1974)?

Verified Customer

Verified customer

Asked: 2019-11-18

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of FLRT3 causing disruption of its protein structure. FLRT3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the FLRT3 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-11-18

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-07-05

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-07-05

Question

Q: if the enzyme conjugated FLRT3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the FLRT3 antigen?

Verified Customer

Verified customer

Asked: 2018-05-17

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-05-17

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2017-07-23

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2017-07-23

Question

Q: Can FLRT3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

T. Lewis

Verified customer

Asked: 2016-02-08

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2016-02-08

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