Anti-EGFR Antibody Picoband™

EGFR antibody

Boster Bio Anti-EGFR Antibody Picoband™ catalog # PB9016. Tested in IF, ICC, WB applications. This antibody reacts with Human, Rat. Cited in 8 publication(s). Independently reviewed in 1 review(s).

Product Info Summary

SKU: PB9016
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: IF, ICC, WB

Product Name

Anti-EGFR Antibody Picoband™

View all EGFR Antibodies

SKU/Catalog Number

PB9016

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-EGFR Antibody Picoband™ catalog # PB9016. Tested in IF, ICC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EGFR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9016)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human EGFR recombinant protein (Position: L25-K346). Human EGFR shares 89% amino acid (aa) sequence identity with mouse EGFR.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9016 is reactive to EGFR in Human, Rat

Applications

PB9016 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

200 kDa

Calculated molecular weight

134.277kDa

Background of EGFR

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For EGFR (Source: Uniprot.org, NCBI)

Gene Name

EGFR

Full Name

Epidermal growth factor receptor

Weight

134.277kDa

Superfamily

protein kinase superfamily

Alternative Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 EGFR ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA epidermal growth factor receptor epidermal growth factor receptor|avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor tyrosine kinase domain|erb-b2 receptor tyrosine kinase 1|proto-oncogene c-ErbB-1|receptor tyrosine-protein kinase erbB-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EGFR, check out the EGFR Infographic

EGFR infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EGFR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9016 has been cited in 8 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Study of the correlation between the expression of nuclear factor kappa B and proliferation regulatory proteins and chronic superficial gastritis,Hu Hui,Ma Zhijian,Ren Shouzhong,Xie Yiqiang, Vojnosanitetski pregled 2020 OnLine-First,00,SP - 135 EP-135
Species: Rat
PB9016 usage in article: APP:IHC, SAMPLE:STOMACH TISSUE, DILUTION:NA

Glucocorticoid mediates prenatal caffeine exposure-induced endochondral ossification retardation and its molecular mechanism in female fetal rats

Anti-tumor activity of erlotinib in the BxPC-3 pancreatic cancer cell line

YC-1 exerts inhibitory effects on MDA-MB-468 breast cancer cells by targeting EGFR in vitro and in vivo under normoxic condition

Gu Y, Zhang S, Wu Q, Xu S, Cui Y, Yang Z, Zhao X, Sun B. J Exp Clin Cancer Res. 2010 Jan 22;29:6. Doi: 10.1186/1756-9966-29-6. Differential Expression Of Decorin, Egfr And Cyclin D1 During Mammary Gland Carcinogenesis In Ta2 Mice With Spontaneous ...

Wang Sb, Cheng Yn, Cui Sx, Zhong Jl, Ward Sg, Sun Lr, Chen Mh, Kokudo N, Tang W, Qu Xj. Clin Exp Metastasis. 2009;26(5):469-77. Doi: 10.1007/S10585-009-9246-Y. Epub 2009 Mar 5. Des-Gamma-Carboxy Prothrombin Stimulates Human Vascular Endothelial Ce...

Yue P, Gao Zh, Xue X, Cui Sx, Zhao Cr, Yuan Y, Yin Z, Inagaki Y, Kokudo N, Tang W, Qu Xj. Eur J Cancer. 2011 May;47(7):1115-24. Doi: 10.1016/J.Ejca.2011.01.017. Epub 2011 Feb 23. Des-??-Carboxyl Prothrombin Induces Matrix Metalloproteinase Activit...

Ge C, Yu M, Petitte Jn, Zhang C. Biol Reprod. 2009 Mar;80(3):528-36. Doi: 10.1095/Biolreprod.108.072728. Epub 2008 Nov 12. Epidermal Growth Factor-Induced Proliferation Of Chicken Primordial Germ Cells: Involvement Of Calcium/Protein Kinase C And ...

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1 Reviews For Anti-EGFR Antibody Picoband™

0

I would highly recommend this Boster antibody...--Elena Deryugina, CELL AND MOLECULAR BIOLOGY, THE SCRIPPS RESEARCH INSTITUTE, ASSISTANT PROFESSOR

Excellent

Source: Biocompare.com

Applications Western Blot
Sample Recombinant N-Terminal domain of human EGFR (aa 1-621) tested at 100 ng/lane
Detection SuperSignal West Pico Chemiluminescent Substrate

"Rabbit antibody PB9016 from Boster was probed for western blot detection of the recombinant N-terminal domain of human EGFR (100 ng/lane). The antibody nicely detected the protein band with the expected mol.wt. of recombinant protein. The antibody appears to be specific since non-specific bands were not detected in parallel lanes with cell lysates lacking EGFR. I would highly recommend this Boster antibody for detection of EGFR with the intact N-terminal domain."

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Order DetailsPrice
PB9016

100μg

$370
PB9016-10ug

10μg sample (liquid)

$99
PB9016-Biotin

100 μg Biotin conjugated

$570
PB9016-Cy3

100 μg Cy3 conjugated

$570
PB9016-Dylight488

100 μg Dylight488 conjugated

$570
PB9016-Dylight550

100 μg Dylight550 conjugated

$570
PB9016-Dylight594

100 μg Dylight594 conjugated

$570
PB9016-FITC

100 μg FITC conjugated

$570
PB9016-HRP

100 μg HRP conjugated

$570
PB9016-APC

100 μg APC conjugated

$670
PB9016-PE

100 μg PE conjugated

$670
PB9016-carrier-free

Carrier Free

$370

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