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Product Info Summary
|Reactive Species:||Human, Rat|
|Application:||IF, ICC, WB|
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Anti-EGFR Antibody Picoband™
Boster Bio Anti-EGFR Antibody Picoband™ catalog # PB9016. Tested in IF, ICC, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EGFR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9016)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived human EGFR recombinant protein (Position: L25-K346). Human EGFR shares 89% amino acid (aa) sequence identity with mouse EGFR.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PB9016 is reactive to EGFR in Human, Rat
PB9016 is guaranteed for IF, ICC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of EGFR using anti-EGFR antibody (PB9016).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA Whole Cell Lysate,
Lane 2: A549 Whole Cell Lysate,
Lane 3: A431 Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR antigen affinity purified polyclonal antibody (Catalog # PB9016) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR at approximately 200KD. The expected band size for EGFR is at 134KD.
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Figure 2. IF analysis of EGFR using anti-EGFR antibody (PB9016).
EGFR was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-EGFR Antibody (PB9016) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Gene/Protein Information For EGFR (Source: Uniprot.Org, NCBI)
Epidermal growth factor receptor
protein kinase superfamily
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 22.214.171.124; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 EGFR ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA epidermal growth factor receptor epidermal growth factor receptor|avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor tyrosine kinase domain|erb-b2 receptor tyrosine kinase 1|proto-oncogene c-ErbB-1|receptor tyrosine-protein kinase erbB-1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on EGFR, check out the EGFR Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for EGFR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PB9016 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Study of the correlation between the expression of nuclear factor kappa B and proliferation regulatory proteins and chronic superficial gastritis,Hu Hui,Ma Zhijian,Ren Shouzhong,Xie Yiqiang, Vojnosanitetski pregled 2020 OnLine-First,00,SP - 135 EP-135
PB9016 usage in article: APP:IHC, SAMPLE:STOMACH TISSUE, DILUTION:NA
Glucocorticoid mediates prenatal caffeine exposure-induced endochondral ossification retardation and its molecular mechanism in female fetal rats
Anti-tumor activity of erlotinib in the BxPC-3 pancreatic cancer cell line
YC-1 exerts inhibitory effects on MDA-MB-468 breast cancer cells by targeting EGFR in vitro and in vivo under normoxic condition
Gu Y, Zhang S, Wu Q, Xu S, Cui Y, Yang Z, Zhao X, Sun B. J Exp Clin Cancer Res. 2010 Jan 22;29:6. Doi: 10.1186/1756-9966-29-6. Differential Expression Of Decorin, Egfr And Cyclin D1 During Mammary Gland Carcinogenesis In Ta2 Mice With Spontaneous ...
Wang Sb, Cheng Yn, Cui Sx, Zhong Jl, Ward Sg, Sun Lr, Chen Mh, Kokudo N, Tang W, Qu Xj. Clin Exp Metastasis. 2009;26(5):469-77. Doi: 10.1007/S10585-009-9246-Y. Epub 2009 Mar 5. Des-Gamma-Carboxy Prothrombin Stimulates Human Vascular Endothelial Ce...
Yue P, Gao Zh, Xue X, Cui Sx, Zhao Cr, Yuan Y, Yin Z, Inagaki Y, Kokudo N, Tang W, Qu Xj. Eur J Cancer. 2011 May;47(7):1115-24. Doi: 10.1016/J.Ejca.2011.01.017. Epub 2011 Feb 23. Des-??-Carboxyl Prothrombin Induces Matrix Metalloproteinase Activit...
Ge C, Yu M, Petitte Jn, Zhang C. Biol Reprod. 2009 Mar;80(3):528-36. Doi: 10.1095/Biolreprod.108.072728. Epub 2008 Nov 12. Epidermal Growth Factor-Induced Proliferation Of Chicken Primordial Germ Cells: Involvement Of Calcium/Protein Kinase C And ...
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