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Product Info Summary
| SKU: | A00135T203 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-eIF4E (T203) Antibody
SKU/Catalog Number
A00135T203
Size
100ul
Form
Liquid
Description
Boster Bio Anti-eIF4E (T203) Antibody catalog # A00135T203. Tested in WB,IHC,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF4E (T203) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00135T203)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 171-210 of Human eIF4E.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00135T203 is reactive to EIF4E in Human, Mouse, Rat
Calculated molecular weight
25.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00135T203 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IP: 1:10-1:100
Validation Images & Assay Conditions
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Western blot (WB) analysis of eIF4E (T203) pAb at 1:1000 dilution Lane1:Hela whole cell lysate(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:The Spleen tissue lysate of Rat(40ug) Lane4:3T3-L1 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)
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Immunohistochemistry (IHC) analyzes of eIF4E (T203) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
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Immunoprecipitation of HEK293T cell lysate using eIF4E (T203) polyclonal antibody (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3).Lane 1 is 30% input.The western blot was probed using eIF4E (T203) #BS3432.“↑”（supernatant）;“↓(deposition）
Specific Publications For Anti-eIF4E (T203) Antibody (A00135T203)
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