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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates.|
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Mouse TREM-1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse TREM-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Trem1 in cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse TREM-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0845)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: A21-S202
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0845 is reactive to Trem1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates.
EK0845 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Trem1
Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide (LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8 (IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1 (MCP1, or SCYA2) and tumor necrosis factor (TNF); MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules (e.g., ITGB1) and costimulatory molecules (e.g., CD40). Trem1 is associated with DAP12 (TYROBP), a molecule frequently associated with activating receptors.
|EK0845-CAP||Anti-Mouse Trem1 Pre-coated 96-well strip microplate||1|
|EK0845-ST||Mouse Trem1 Standard||2 vials, 10 ng/tube|
|EK0845-DA||Mouse Trem1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse TREM-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Trem1, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse TREM-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Trem1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Trem1 (Source: Uniprot.Org, NCBI)
Triggering receptor expressed on myeloid cells 1
CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1 Trem1|triggering receptor expressed on myeloid cells 1|triggering receptor expressed on myeloid cells 1|triggering receptor expressed in monocytes 1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Trem1, check out the Trem1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Trem1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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