Click image to see more details
Product Info Summary
| SKU: | A30789 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-ELTD1 Antibody
SKU/Catalog Number
A30789
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ELTD1 Antibody (Catalog# A30789). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ELTD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30789)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ELTD1. Immunogen sequence location: 251 - 300
Reactive Species
A30789 is reactive to ADGRL4 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
77.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30789 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of LOVO cells, using ELTD1 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-ELTD1 Antibody (A30789)
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Customer Reviews
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6 Customer Q&As for Anti-ELTD1 Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for visceral pleura using anti-ELTD1 antibody A30789. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-09
Question
I was wanting to use your anti-ELTD1 antibody for IF for human visceral pleura on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human visceral pleura identification?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
You can see on the product datasheet, A30789 anti-ELTD1 antibody has been validated for IF on human tissues. We have an innovator award program that if you test this antibody and show it works in human visceral pleura in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-09
Question
See attached the WB image, lot number and protocol we used for visceral pleura using anti-ELTD1 antibody A30789. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-17
Question
We are currently using anti-ELTD1 antibody A30789 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
The anti-ELTD1 antibody (A30789) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-17
Question
Is this A30789 anti-ELTD1 antibody reactive to the isotypes of ADGRL4?
Verified Customer
Verified customer
Asked: 2018-06-27
Answer
The immunogen of A30789 anti-ELTD1 antibody is A peptide derived from human ELTD1. Immunogen sequence location: 251 - 300. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-27
Question
Would A30789 anti-ELTD1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-27
Answer
As indicated on the product datasheet, A30789 anti-ELTD1 antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-27
