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Product Info Summary
| SKU: | A11133 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ZBED1 Antibody
SKU/Catalog Number
A11133
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZBED1 Antibody (Catalog # A11133). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
ZBED1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZBED1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11133)
Host
Rabbit
Contents
ZBED1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZBED1 antibody was raised against a 15 amino acid synthetic peptide from near the center of human ZBED1. The immunogen is located within amino acids 180 - 230 of ZBED1.
Cross-reactivity
This antibody is predicted to have no cross-reactivity to ZBED2 or ZBED3.
Reactive Species
A11133 is reactive to ZBED1 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
78.2 kDa
Background of ZBED1
ZBED1, also known as DREF, is the human homolog of the Drosophila protein DREF, a transcriptional regulatory factor required for expression of genes involved in DNA replication and cell proliferation. RNA interference (RNAi) experiments targeting ZBED1 RNA resulted in the inhibition of S phase entry and reduction of histone H1 mRNA in HeLa cells, suggesting that ZBED1 also plays a role in cell proliferation in human cells. At least 22 ribosomal proteins (RPs) possess ZBED1 binding sites in their promoters; anti-ZBED1 RNAi also resulted in decreased RP gene expression. Immunoprecipitation analyses have shown that ZBED1 self-associates in vivo via a carboxyl-terminal hATC domain, and this self-association is required for nuclear localization and DNA binding.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11133 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZBED1 antibody can be used for detection of ZBED1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZBED1 in A549 cell lysate with ZBED1 antibody at 1 μg/mL.
Specific Publications For Anti-ZBED1 Antibody (A11133)
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