Anti-eNOS/NOS3 (Phospho-S1177) Antibody (Monoclonal, 34N82)

Product Name

Anti-eNOS/NOS3 (Phospho-S1177) Antibody (Monoclonal, 34N82)

View all NOS3 Antibodies

SKU/Catalog Number

M01604-3

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-eNOS/NOS3 (Phospho-S1177) Antibody (Monoclonal, 34N82) catalog # M01604-3. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-eNOS/NOS3 (Phospho-S1177) Antibody (Monoclonal, 34N82) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01604-3)

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

34N82

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser1177 of human eNOS.

Reactive Species

M01604-3 is reactive to NOS3 in Human, Mouse, Rat

Calculated molecular weight

133.3 kDa

Background of NOS3

NOS3(Nitric Oxide Synthase 3), also called ENOS, a?nitric oxide synthase?that generates?NO?in?blood vessels?and is involved with regulating?vascular tone?by inhibiting?smooth muscle?contraction and platelet?aggregation. The NOS3 gene is mapped on 7q36.1. Variations in this gene are associated with susceptibility to?coronary spasm. ?Fulton et al.?(1999) concluded the eNOS is an AKT substrate linking signal transduction by AKT to the release of the gaseous second messenger nitric oxide. ?AKT mediates the activation of eNOS, leading to increased nitric oxide production. Inhibition of the PI3K AKT pathway or mutation of the AKT site on eNOS protein at serine-1177 attenuated the serine phosphorylation and prevented the activation of eNOS. RT-PCR analysis showed that expression of?NOS3?in human umbilical vein endothelial cells (HUVECs) and human aortic vascular smooth muscle cells (HAOVSMCs) was inversely proportional to that of NOS3AS.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01604-3 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 1:500-2000
Immunohistochemistry, 1:50-200

Validation Images & Assay Conditions

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See full target information NOS3

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