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Product Info Summary
| SKU: | A01438 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Eotaxin CCL11 Antibody
SKU/Catalog Number
A01438
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Eotaxin CCL11 Antibody (Catalog # A01438). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
Eotaxin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Eotaxin CCL11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01438)
Host
Rabbit
Contents
Eotaxin Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Eotaxin antibody was raised in rabbits against a peptide corresponding to amino acids near the carboxy terminus of human eotaxin. The immunogen is located within the last 50 amino acids of Eotaxin.
Reactive Species
A01438 is reactive to CCL11 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
10.7 kDa
Background of CCL11
Chemokines play a key role in inflammation. The CC chemokine eotaxin is a potent and specific eosinophil chemoattractant that is expressed by a variety of cell types in certain inflammatory conditions. Some G-protein coupled chemokine receptors are also utilized as virus coreceptors for fusion and infection of cells. The eotaxin receptor CCR3 is required for HIV-1 entry into target cells such as microglia and eotaxin inhibits the infection of HIV-1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01438 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Eotaxin antibody can be used for detection of eotaxin by Western blot at 1 μg/mL. The recombinant protein can be used as positive control and monomer and homodimer are detected at approximate 9 and 18 kDa. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of eotaxin in HeLa cell lysate containing 10, 2.5, or 1 ng of full length recombinant eotaxin with eotaxin antibody at 1 μg/mL.
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Immunocytochemistry of Eotaxin in 293 cells with Eotaxin antibody at 5 μg/mL.
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Immunofluorescence of Eotaxin in 293 cells with Eotaxin antibody at 10 μg/mL.
Specific Publications For Anti-Eotaxin CCL11 Antibody (A01438)
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