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Product Info Summary
| SKU: | M00388-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-ERCC1 Mouse Monoclonal Antibody [Clone ID: OTI1A3]
SKU/Catalog Number
M00388-1
Size
100 µl
Description
Boster Bio Anti-ERCC1 mouse monoclonal antibody, clone OTI1A3 (formerly 1A3). Catalog# M00388-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ERCC1 Mouse Monoclonal Antibody [Clone ID: OTI1A3] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00388-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1A3
Isotype
IgG1
Immunogen
Full-length protein expressed in 293T cell transfected with human ERCC1 expression vector
Reactive Species
M00388-1 is reactive to ERCC1 in Human, Mouse
Observed Molecular Weight
32.6 kDa
Calculated molecular weight
32.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00388-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC: 1:50
IF: 1:100
Flow cytometry: 1:100
Validation Images & Assay Conditions
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HEK293T cells transfected with either pCMV6-ENTRY ERCC1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-ERCC1 mouse monoclonal (M00388-1
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Anti-ERCC1 mouse monoclonal antibody (M00388-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1.
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Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded bladder tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded thyroid tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
Specific Publications For Anti-ERCC1 Mouse Monoclonal Antibody [Clone ID: OTI1A3] (M00388-1)
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