Click image to see more details
Product Info Summary
| SKU: | A01993 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-ERCC4/Xpf Antibody
SKU/Catalog Number
A01993
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ERCC4/Xpf Antibody catalog # A01993. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ERCC4/Xpf Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01993)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human XPF. AA range:801-850
Reactive Species
A01993 is reactive to ERCC4 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
104.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01993 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of various cells using ERCC4 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
Click image to see more details
Western Blot analysis of 293 cells using ERCC4 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
Click image to see more details
Western blot analysis of lysates from 293 cells, using XPF Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-ERCC4/Xpf Antibody (A01993)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-ERCC4/Xpf Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-ERCC4/Xpf Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-ERCC4/Xpf Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adrenal tissue using anti-ERCC4/Xpf antibody A01993. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-23
Question
We are currently using anti-ERCC4/Xpf antibody A01993 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
The anti-ERCC4/Xpf antibody (A01993) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-06
Question
Would A01993 anti-ERCC4/Xpf antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
It shows on the product datasheet, A01993 anti-ERCC4/Xpf antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-13
Question
Would anti-ERCC4/Xpf antibody A01993 work on goat WB with fibroblast?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
Our lab technicians have not tested anti-ERCC4/Xpf antibody A01993 on goat. You can run a BLAST between goat and the immunogen sequence of anti-ERCC4/Xpf antibody A01993 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat fibroblast in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-02
