Product Info Summary
SKU: | A01993 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-ERCC4/Xpf Antibody
SKU/Catalog Number
A01993
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ERCC4/Xpf Antibody catalog # A01993. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ERCC4/Xpf Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01993)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the C-terminal region of human ERCC4. at AA rangle: 770-850
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01993 is reactive to ERCC4 in Human, Mouse
Applications
A01993 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
104.486kDa
Background of XPF
The PKR-like endoplasmic reticulum kinase (PERK, also know as Eukaryotic translation initiation factor 2-alpha kinase 3) is a type I transmembrane protein localized to the endoplasmic reticulum (ER). PERK consists of an N-terminal ER luminal domain, a membrane-spanning region, and a cytosolic C-terminal serine/threonine kinase domain (1). The luminal domain of PERK is bound to the ER chaperone GRP78 in unstressed cells (2). PERK activation occurs upon accumulation of misfolded proteins and the ER lumen, which triggers GRP78 dissociation from PERK thereby allowing PERK dimerization and autophosphorylation (3, 4). PERK phosphorylates two established targets: the eukaryotic translation initiation factor 2 alpha (eIF2, (1)) and the Nrf2 transcription factor (5). Phosphorylation of eIF2 results in attenuation of translation initiation (6). The translational block also contributes to cell cycle arrest due to loss of the G1 regulatory protein, cyclin D1 (7). PERK-dependent phosphorylation of Nrf2 promotes transcription of phase II detoxifying enzymes which is critically important for elimination of intracellular reactive oxygen species (8). Thus, while inhibiting new protein synthesis and thereby decreasing the ER protein load PERK simultaneously induces expression of genes that help restore cellular redox homeostasis and promote survival.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of 293 cells using ERCC4 Polyclonal antibody.
Click image to see more details
Western Blot (WB) analysis of specific cells using ERCC4 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For ERCC4 (Source: Uniprot.org, NCBI)
Gene Name
ERCC4
Full Name
DNA repair endonuclease XPF
Weight
104.486kDa
Superfamily
XPF family
Alternative Names
DNA excision repair protein ERCC-4; DNA repair protein complementing XP-F cells; EC 3.1; ERCC11; ERCC4; excision repair cross-complementing rodent repair deficiency, complementationgroup 4; xeroderma pigmentosum, complementation group F; XPF; XPFcomplementing defective, in Chinese hamster ERCC4 ERCC11, FANCQ, RAD1, XFEPS, XPF ERCC excision repair 4, endonuclease catalytic subunit DNA repair endonuclease XPF|DNA excision repair protein ERCC-4|DNA repair protein complementing XP-F cells|excision repair cross-complementation group 4|excision repair cross-complementing rodent repair deficiency, complementation group 4|excision-repair, complementing defective, in Chinese hamster|xeroderma pigmentosum group F-complementing protein|xeroderma pigmentosum, complementation group F
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERCC4, check out the ERCC4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERCC4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ERCC4/Xpf Antibody (A01993)
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No publications found for A01993
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-ERCC4/Xpf Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adrenal tissue using anti-ERCC4/Xpf antibody A01993. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-23
Question
We are currently using anti-ERCC4/Xpf antibody A01993 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
The anti-ERCC4/Xpf antibody (A01993) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-06
Question
Would A01993 anti-ERCC4/Xpf antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
It shows on the product datasheet, A01993 anti-ERCC4/Xpf antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-13
Question
Would anti-ERCC4/Xpf antibody A01993 work on goat WB with fibroblast?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
Our lab technicians have not tested anti-ERCC4/Xpf antibody A01993 on goat. You can run a BLAST between goat and the immunogen sequence of anti-ERCC4/Xpf antibody A01993 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat fibroblast in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-02