MAPK3 antibody (PA1343) performs well in IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
  • Over 2 Publications
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Anti-ERK1/MAPK3 Antibody Picoband®

MAPK3 antibody

Reactive SpeciesHostApplication
Human, Mouse, RatRabbitIHC, ICC, WB

SKU: PA1343

Size: 100 μg

Bundle to save: I do not want free secondary ab
Price:
$370/100 μg

3+ in stock in USA, Ships Next Business Day.

Quantity: 1

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Tested Applications

MAPK3 antibody was positively detected by WB in:

Rat Spleen Tissue, Rat Thymus Tissue, Rat Skeletal Muscle Tissue, Rat Kidney Tissue, HELA Cell, JURKAT Cell, RAJI Cell, COLO320 Cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

MAPK3 antibody was positively detected by IHC in:

Human Mammary Cancer Tissue, rat intestine tissue

Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

MAPK3 antibody was positively detected by ICC in:

HELA Cell

Recommend Dilution

Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Rat, Mouse
Immunocytochemistry
0.5-1μg/ml
Human, Mouse, Rat
Western blot
0.1-0.5μg/ml
Human, Rat, Mouse

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Reactive Data

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WB
IHC
IF
Rat
Tested
Tested
Recommend
Human
Tested
Recommend
Mouse
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted

Product Information

Product Name
Anti-ERK1/MAPK3 Antibody Picoband®
View all MAPK3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human ERK1, identical to the related mouse and rat sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated Molecular Weight

43.1 kDa

Background of MAPK3
Mitogen-activated protein kinase 3 is an enzyme that in humans is encoded by the MAPK3 gene.The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. MAPK3 gene is mapped to human chromosome 16 by hybrid cell panel analysis.
Cite This Product
Anti-ERK1/MAPK3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1343)

Protocols

Western Blot protocol for MAPK3 antibody
Immunohistochemistry (IHC) protocol for MAPK3 antibody
Immunocytochemistry (ICC) protocol for MAPK3 antibody

Publications

Application:
Species:
Effects of microRNA-133b on retinal vascular endothelial cell proliferation and apoptosis through angiotensinogen-mediated angiotensin II- extracellular signal-regulated kinase 1/2 signalling pathway in rats with diabetic retinopathy
Published: 2018-02-28
Journal: ACTA OPHTHALMOLOGICA
Application: WB
Impact Factor: 3.32
Species: Rat
PMID: 29488353
Allicin inhibits tubular epithelial‑myofibroblast transdifferentiation under high glucose conditions in vitro
Published: 2016-11-18
Journal: Experimental and Therapeutic Medicine
Application: WB
Impact Factor: 1.28
Species: Human
PMID: 28123498

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Product Promise

We promise Anti-ERK1/MAPK3 Antibody Picoband® performs excellently in relevant applications for IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more

In stock
Order Product
PA1343
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Oct 16, 2026