Anti-ERK1/MAPK3 Antibody

Boster Bio Anti-ERK1/MAPK3 Antibody catalog # PA1343. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PA1343
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, ICC, WB

Product Name

Anti-ERK1/MAPK3 Antibody

See all MAPK3 products

SKU/Catalog Number

PA1343

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ERK1/MAPK3 Antibody catalog # PA1343. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ERK1/MAPK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1343)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human ERK1 (287-301aa KTKVAWAKLFPKSDS), identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1343 is reactive to MAPK3 in Human, Mouse, Rat

Applications

PA1343 is guaranteed for IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For MAPK3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P27361

Gene ID

5595

Gene Name

MAPK3

Full Name

Mitogen-activated protein kinase 3

Weight

43.136kDa

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.11; ERK1; ERK-1; ERK1p44-MAPK; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK3; MGC20180; Microtubule-associated protein 2 kinase; Mitogen-activated protein kinase 1; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; p44mapk; PRKM3; PRKM3EC 2.7.11.24 MAPK3 ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK mitogen-activated protein kinase 3 mitogen-activated protein kinase 3|MAPK 1|extracellular signal-regulated kinase 1|extracellular signal-related kinase 1|insulin-stimulated MAP2 kinase|microtubule-associated protein 2 kinase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MAPK3, check out the MAPK3 Infographic

MAPK3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MAPK3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1343 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Inhibition of extracellular signal-regulated kinases ameliorates hypertension-induced renal vascular remodeling in rat models

Synthesis and biological evaluation of novel N, N%u2032-disubstituted urea and thiourea derivatives as potential anti-melanoma agents

Disulfiram inhibits TGF-?-induced epithelial-mesenchymal transition and stem-like features in breast cancer via ERK/NF-?B/Snail pathway

Fructus phyllanthi tannin fraction induces apoptosis and inhibits migration and invasion of human lung squamous carcinoma cells in vitro via MAPK/MMP pathways

Yang Sl, Han R, Liu Y, Hu Ly, Li Xl, Zhu Ly. Wound Repair Regen. 2014 Jul-Aug;22(4):548-54. Doi: 10.1111/Wrr.12195. Negative Pressure Wound Therapy Is Associated With Up-Regulation Of Bfgf And Erk1/2 In Human Diabetic Foot Wounds.

Ma Hr, Wang J, Chen Yf, Chen H, Wang Ws, Aisa Ha. Int J Mol Med. 2014 Jun;33(6):1627-34. Doi: 10.3892/Ijmm.2014.1722. Epub 2014 Apr 3. Icariin And Icaritin Stimulate The Proliferation Of Skbr3 Cells Through The Gper1-Mediated Modulation Of The Egf...

Hu Cp, Feng Jt, Tang Yl, Zhu Jq, Lin Mj, Yu Me. Mediators Inflamm. 2006;2006(5):84829. Lif Upregulates Expression Of Nk-1R In Nhbe Cells.

Liu Y, Zhu Z, Xiong Z, Zheng J, Hu Z, Qiu J. Int J Clin Exp Pathol. 2014 Aug 15;7(9):5750-61. Ecollection 2014. Knockdown Of Protein Tyrosine Phosphatase Receptor U Inhibits Growth And Motility Of Gastric Cancer Cells.

Tang Y, Zhang X, Qi F, Chen M, Li Y, Liu L, He W, Li Z, Zu X. Exp Ther Med. 2015 May;9(5):1851-1856. Epub 2015 Feb 25. Afatinib Inhibits Proliferation And Invasion And Promotes Apoptosis Of The T24 Bladder Cancer Cell Line.

Li K, Zhu Zc, Liu Yj, Liu Jw, Wang Ht, Xiong Zq, Shen X, Hu Zl, Zheng J. Int J Clin Exp Pathol. 2013 Sep 15;6(11):2460-7. Ecollection 2013. Zfx Knockdown Inhibits Growth And Migration Of Non-Small Cell Lung Carcinoma Cell Line H1299.

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16 Customer Q&As for Anti-ERK1/MAPK3 Antibody

Question

Is a blocking peptide available for product anti-ERK1/MAPK3 antibody (PA1343)?

Verified Customer

Verified customer

Asked: 2020-03-09

Answer

We do provide the blocking peptide for product anti-ERK1/MAPK3 antibody (PA1343). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-09

Question

We have observed staining in rat right frontal lobe. What should we do? Is anti-ERK1/MAPK3 antibody supposed to stain right frontal lobe positively?

Verified Customer

Verified customer

Asked: 2020-02-05

Answer

According to literature right frontal lobe does express MAPK3. According to Uniprot.org, MAPK3 is expressed in right frontal lobe, hepatoma, lymph, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MAPK3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Hepatoma, Pubmed ID: 1540184, 7687743
Leukemic T-cell, Pubmed ID: 19690332
Lymph, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-02-05

Question

We are currently using anti-ERK1/MAPK3 antibody PA1343 for mouse tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-15

Answer

The anti-ERK1/MAPK3 antibody (PA1343) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-15

Question

Will anti-ERK1/MAPK3 antibody PA1343 work on goat WB with lymph?

Verified Customer

Verified customer

Asked: 2019-11-22

Answer

Our lab technicians have not tested anti-ERK1/MAPK3 antibody PA1343 on goat. You can run a BLAST between goat and the immunogen sequence of anti-ERK1/MAPK3 antibody PA1343 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat lymph in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-22

Question

We need to test anti-ERK1/MAPK3 antibody PA1343 on rat cervix carcinoma for research purposes, then I may be interested in using anti-ERK1/MAPK3 antibody PA1343 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-30

Answer

The products we sell, including anti-ERK1/MAPK3 antibody PA1343, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-30

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-ERK1/MAPK3 antibody PA1343. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-27

Question

I have a question about product PA1343, anti-ERK1/MAPK3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1343 anti-ERK1/MAPK3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-27

Question

I was wanting to use your anti-ERK1/MAPK3 antibody for IHC for rat cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

As indicated on the product datasheet, PA1343 anti-ERK1/MAPK3 antibody has been validated for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-24

Question

I see that the anti-ERK1/MAPK3 antibody PA1343 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-05-16

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-05-16

Question

Would anti-ERK1/MAPK3 antibody PA1343 work for IHC with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2019-02-15

Answer

According to the expression profile of cervix carcinoma, MAPK3 is highly expressed in cervix carcinoma. So, it is likely that anti-ERK1/MAPK3 antibody PA1343 will work for IHC with cervix carcinoma.

Boster Scientific Support

Answered: 2019-02-15

Question

We ordered your anti-ERK1/MAPK3 antibody for IHC on cervix carcinoma in a previous project. I am using rat, and I plan to use the antibody for ICC next. My question regards examining cervix carcinoma as well as hepatoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?

D. Johnson

Verified customer

Asked: 2019-02-08

Answer

I looked at the website and datasheets of our anti-ERK1/MAPK3 antibody and it appears that PA1343 has been validated on rat in both IHC and ICC. Thus PA1343 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-02-08

Question

My colleagues were well pleased with the WB result of your anti-ERK1/MAPK3 antibody. However we have seen positive staining in cervix carcinoma erythroleukemia caveola using this antibody. Is that expected? Could you tell me where is MAPK3 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-04-26

Answer

From literature, cervix carcinoma erythroleukemia does express MAPK3. Generally MAPK3 expresses in cytoplasm. nucleus. membrane, caveola. Regarding which tissues have MAPK3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Hepatoma, Pubmed ID: 1540184, 7687743
Leukemic T-cell, Pubmed ID: 19690332
Lymph, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-04-26

Question

Please see the WB image, lot number and protocol we used for cervix carcinoma using anti-ERK1/MAPK3 antibody PA1343. Please let me know if you require anything else.

R. Huang

Verified customer

Asked: 2017-01-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-01-13

Question

Will PA1343 anti-ERK1/MAPK3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

P. Krishna

Verified customer

Asked: 2014-04-18

Answer

You can see on the product datasheet, PA1343 anti-ERK1/MAPK3 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-04-18

Question

Is this PA1343 anti-ERK1/MAPK3 antibody reactive to the isotypes of MAPK3?

J. Johnson

Verified customer

Asked: 2014-01-06

Answer

The immunogen of PA1343 anti-ERK1/MAPK3 antibody is A synthetic peptide corresponding to a sequence in the middle region of human ERK1(287-301aa KTKVAWAKLFPKSDS), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-01-06

Question

Is there a BSA free version of anti-ERK1/MAPK3 antibody PA1343 available?

B. Parker

Verified customer

Asked: 2013-03-06

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ERK1/MAPK3 antibody PA1343 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-03-06

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