Anti-Esrp-1/2 Esrp2 Monoclonal Antibody

Product Name

Anti-Esrp-1/2 Esrp2 Monoclonal Antibody

View all ESRP1/ESRP2 Antibodies

SKU/Catalog Number

M06068-4

Size

25uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Esrp-1/2 Esrp2 Monoclonal Antibody (Catalog # M06068-4). Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. Expiration date is three (3) months from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Esrp-1/2 Esrp2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06068-4)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 23A7.C9

Isotype

IgG2b kappa

Immunogen

Anti-Esrp-1/2 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.

Reactive Species

M06068-4 is reactive to ESRP1/ESRP2 in Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

77.4 kDa

Background of ESRP1/ESRP2

Epithelial splicing regulatory protein 1 (Esrp-1) and Esrp-2 are mRNA splicing factors that regulate the formation of epithelial cell-specific isoforms. They specifically regulate the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-1 and -2 act by directly binding specific sequences in mRNAs. They bind the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M06068-4 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:750,000
WB: 1:1000
This protein-A purified antibody has been tested for use western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 75.5 kDa in size corresponding to Esrp-1 and 77.4 kDa in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract.

Validation Images & Assay Conditions

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See full target information ESRP1/ESRP2

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