Anti-Esrp-1/2 Monoclonal Antibody

Product Name

Anti-Esrp-1/2 Monoclonal Antibody

View all ESRP1/ESRP2 Antibodies

SKU/Catalog Number

M06068

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Esrp-1/2 Monoclonal Antibody (Catalog # M06068). Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Esrp-1/2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06068)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 23A7.C9 IgG1

Isotype

IgG1

Immunogen

Anti-Esrp-1/2 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.

Reactive Species

M06068 is reactive to ESRP1/ESRP2 in Mouse

Calculated molecular weight

77.4 kDa

Background of ESRP1/ESRP2

Epithelial splicing regulatory protein-1 (Esrp-1) and Esrp-2 are mRNA splicing factors that regulate the formation of epithelial cell-specific isoforms. They specifically regulate the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-1 and -2 act by directly binding specific sequences in mRNAs. They bind the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M06068 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:750,000
WB: 1:1000
This protein-A purified antibody has been tested for use western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 75.5 kDa in size corresponding to Esrp-1 and 77.4 kDa in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract.

Validation Images & Assay Conditions

See full target information ESRP1/ESRP2

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