Anti-Fascin/FSCN1 Antibody

Boster Bio Anti-Fascin/FSCN1 Antibody catalog # PA1575. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PA1575
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Fascin/FSCN1 Antibody

See all Fascin products

SKU/Catalog Number

PA1575

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Fascin/FSCN1 Antibody catalog # PA1575. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Fascin/FSCN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1575)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Fascin (97-113aa DDGRWSLQSEAHRRYFG), identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1575 is reactive to FSCN1 in Human, Mouse, Rat

Applications

PA1575 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For FSCN1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q16658

Gene ID

6624

Gene Name

FSCN1

Full Name

Fascin

Weight

54.53kDa

Superfamily

fascin family

Alternative Names

FAN1; FAN1,55 kDa actin-bundling protein; fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus); Fascin; FLJ38511; FSCN1; HSN; p55actin bundling protein; singed (Drosophila)-like (sea urchin fascin homolog like); singed-like (fascin homolog, sea urchin); Singed-like Protein; SNL; SNLfascin FSCN1 FAN1, HSN, SNL, p55 fascin actin-bundling protein 1 fascin|55 kDa actin-bundling protein|epididymis secretory sperm binding protein|fascin homolog 1, actin-bundling protein|singed-like (fascin homolog, sea urchin)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FSCN1, check out the FSCN1 Infographic

FSCN1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FSCN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1575 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Jiang L, Wang P, Chen H. Ups J Med Sci. 2014 Nov;119(4):324-32. Doi: 10.3109/03009734.2014.960053. Epub 2014 Sep 18. Overexpression Of Foxm1 Is Associated With Metastases Of Nasopharyngeal Carcinoma.

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4 Customer Q&As for Anti-Fascin/FSCN1 Antibody

Question

We were well pleased with the WB result of your anti-Fascin/FSCN1 antibody. However we have observed positive staining in cervix carcinoma cytosol using this antibody. Is that expected? Could you tell me where is FSCN1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-02-21

Answer

Based on literature, cervix carcinoma does express FSCN1. Generally FSCN1 expresses in cytoplasm, cytosol. Regarding which tissues have FSCN1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 3525578
Brain, Eye, Lung, and Muscle, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Teratocarcinoma, Pubmed ID: 8068206
Testis, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-02-21

Question

We are currently using anti-Fascin/FSCN1 antibody PA1575 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-13

Answer

The anti-Fascin/FSCN1 antibody (PA1575) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-13

Question

We have seen staining in rat cervix carcinoma. What should we do? Is anti-Fascin/FSCN1 antibody supposed to stain cervix carcinoma positively?

R. Zhang

Verified customer

Asked: 2018-06-15

Answer

According to literature cervix carcinoma does express FSCN1. According to Uniprot.org, FSCN1 is expressed in epithelium of bronchus, teratocarcinoma, testis, brain, eye, lung muscle, ovarian carcinoma, brain, cajal-retzius cell fetal brain cortex, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have FSCN1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 3525578
Brain, Eye, Lung, and Muscle, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Teratocarcinoma, Pubmed ID: 8068206
Testis, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-06-15

Question

Our lab want to know about using your anti-Fascin/FSCN1 antibody for cell motility studies. Has this antibody been tested with western blotting on a549 whole cell lysate? We would like to see some validation images before ordering.

S. Johnson

Verified customer

Asked: 2017-04-19

Answer

Thanks for your inquiry. This PA1575 anti-Fascin/FSCN1 antibody is tested on u87 whole cell lysate, a549 whole cell lysate, ht1080 whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-04-19

Size

Total: $315

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PA1575
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