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Product Info Summary
| SKU: | M12412-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, WB |
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Product info
Product Name
Anti-FATE1 Mouse Monoclonal Antibody [Clone ID: OTI6F10]
SKU/Catalog Number
M12412-2
Size
100 µl
Description
Boster Bio FATE1 mouse monoclonal antibody, clone OTI6F10 (formerly 6F10). Catalog# M12412-2. Tested in IF, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-FATE1 Mouse Monoclonal Antibody [Clone ID: OTI6F10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12412-2)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI6F10
Isotype
IgG1
Immunogen
Full length human recombinant protein of human FATE1 (NP_001756) produced in HEK293T cell.
Reactive Species
M12412-2 is reactive to FATE1 in Dog, Human, Monkey, Mouse, Rat
Calculated molecular weight
20.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M12412-2 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:200~4000
IF 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FATE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FATE1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FATE1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Anti-FATE1 mouse monoclonal antibody (M12412-2) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FATE1.
Specific Publications For Anti-FATE1 Mouse Monoclonal Antibody [Clone ID: OTI6F10] (M12412-2)
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