|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-KIN/Kin17 Antibody|
|Description||Rabbit IgG polyclonal antibody for DNA/RNA-binding protein KIN17(KIN) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-KIN/Kin17 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2238)|
|Replacement Item||This antibody may replace the following items: sc-240731|sc-240733|sc-26955|sc-26955-X|sc-271753|sc-27970|sc-292595|sc-32768|sc-32768-X|sc-32769|sc-32769-X|sc-33136|sc-373787|sc-514471|sc-515104|sc-70195|sc-70196|sc-70197|sc-81795|sc-98851 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human KIN(48-64aa ESHQRQLLLASENPQQF), identical to the related mouse and rat sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||DNA/RNA-binding protein KIN17|
|Molecular Weight||45374 MW|
|Protein Function||Involved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo (By similarity). Binds via its C-terminal domain to RNA in vitro. .|
|Tissue Specificity||Ubiquitously expressed in all tissues examined, with highest levels in skeletal muscle, heart and testis. Differentially expressed in non-tumorigenic and tumorigenic cell lines. Highly expressed in proliferating epithelial keratinocyte cells in vitro (at protein level). .|
|Sequence Similarities||Belongs to the KIN17 family.|
|Subcellular Localization||Nucleus . Cytoplasm . During S phase, strongly associated with the nuclear matrix, and to chromosomal DNA in the presence of DNA damage. Also shows cytoplasmic localization in elongated spermatids. .|
|Alternative Names||DNA/RNA-binding protein KIN17;Binding to curved DNA;KIN, antigenic determinant of recA protein homolog;KIN ;BTCD, KIN17;|
|Research Areas|||epigenetics and nuclear signaling|dna / rna|dna damage & repair|dna damage response|dna damage recognition||
Background for DNA/RNA-binding protein KIN17
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-KIN/Kin17 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-KIN/Kin17 Antibody Images
Click the images to enlarge.
Lane 1: Rat Skeletal Muscle Tissue Lysate
Lane 2: Human Placenta Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,