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Product Info Summary
| SKU: | M13796 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-FBXO42 Mouse Monoclonal Antibody [Clone ID: OTI1H4]
SKU/Catalog Number
M13796
Size
100 µl
Description
Boster Bio FBXO42 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4). Catalog# M13796. Tested in FC, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-FBXO42 Mouse Monoclonal Antibody [Clone ID: OTI1H4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13796)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1H4
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 356-717 of human FBXO42 (NP_061867) produced in E.coli.
Reactive Species
M13796 is reactive to FBXO42 in Human, Mouse, Rat
Calculated molecular weight
77.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M13796 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
IHC 1:150
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXO42 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXO42.
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FBXO42 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FBXO42 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FBXO42 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FBXO42 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either FBXO42 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FBXO42 antibody (M13796)
Specific Publications For Anti-FBXO42 Mouse Monoclonal Antibody [Clone ID: OTI1H4] (M13796)
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