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Product Info Summary
| SKU: | M05418 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-ATP2B3 Antibody (C-term)
SKU/Catalog Number
M05418
Size
80 µl
Description
Boster Bio Anti-ATP2B3 Antibody (C-term) (Catalog # M05418). Tested in WB, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ATP2B3 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05418)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ATP2B3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1161-1194 amino acids from the C-terminal region of human ATP2B3.
Reactive Species
M05418 is reactive to ATP2B3 in Human
Calculated molecular weight
134.2 kDa
Background of ATP2B3
Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05418 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling ATP2B3 with M05418 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing membrane staining on Hela cell line.The nuclear counter stain is DAPI (blue).
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Anti-ATP2B3 Antibody (C-term)at 1:2000 dilution + U-87 MG whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 134 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-ATP2B3 Antibody (C-term) (M05418)
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