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- Table of Contents
FGB antibody (M01204-1) performs well in IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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IHC analysis of Fibrinogen beta chain/FGB using anti-Fibrinogen beta chain/FGB antibody (M01204-1).
Fibrinogen beta chain/FGB was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Fibrinogen beta chain/FGB Antibody (M01204-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Fibrinogen beta chain/FGB was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Fibrinogen beta chain/FGB Antibody (M01204-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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Anti-Fibrinogen beta chain/FGB Antibody Picoband® (monoclonal, 6D12)
FGB antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | IHC, WB |
Tested Applications
FGB antibody was positively detected by WB in:
human HEPG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FGB antibody was positively detected by IHC in:
human liver cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Fibrinogen beta chain/FGB Antibody Picoband® (monoclonal, 6D12)
View all FGB Antibodies
View all FGB Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Monoclonal
Clone Number
6D12
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human FGB, different from the related mouse sequence by three amino acids, and from the related rat sequence by five amino acids.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
56 kDa
Calculated Molecular Weight
55.9 kDa
Background of FGB
Fibrinogen beta chain, mapped to 4q31.3, is also known asFGB. The protein encoded by this gene is the beta component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Cite This Product
Anti-Fibrinogen beta chain/FGB Antibody Picoband® (monoclonal, 6D12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01204-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Dog
97%
High Homology
Cow
94%
High Homology
Mouse
91%
High Homology
Rabbit
91%
High Homology
Sheep
91%
High Homology
Rat
85%
Moderate Homology
Zebrafish
36%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for FGB antibody
Immunohistochemistry (IHC) protocol for FGB antibody
Publications
Application:
Species:
Histological analysis of thrombus composition in acute ischemic stroke with large vessel occlusion: impact of r-tPA treatment
Published: 2025-11-21
Journal: Frontiers in Neuroscience
Application: IHC
Impact Factor: 3.20
Species: Human
PMID: 41357834
Glaesserella parasuis induces tissue transglutaminase-mediated fibrinogen crosslinking through NF-κB activation
Published: 2025-01-28
Journal: VETERINARY MICROBIOLOGY
Application: IF
Impact Factor: 2.70
Species: Pig
PMID: 39919498
Pleural thickening induced by Glaesserella parasuis infection was linked to increased collagen and elastin
Published: 2022-08-11
Journal: Frontiers in Cellular and Infection Microbiology
Application: IF
Impact Factor: 6.07
Species: Piglet
PMID: 36034702
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Product Promise
We promise Anti-Fibrinogen beta chain/FGB Antibody Picoband® (monoclonal, 6D12) performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01204-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026