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Product Info Summary
| SKU: | PA1477 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-FGFR1 Antibody Picoband®
SKU/Catalog Number
PA1477
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FGFR1 Antibody catalog # PA1477. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGFR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1477)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human FGFR1, different from the related mouse sequence by two amino acids and rat sequence by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1477 is reactive to FGFR1 in Human, Mouse, Rat
Observed Molecular Weight
92 kDa
Calculated molecular weight
91.9 kDa
Background of FGFR1
FGFR1, Fibroblast growth factor receptor 1, also known as basic fibroblast growth factor receptor 1, fms-related tyrosine kinase-2/Pfeiffer syndrome, and CD331, is a receptor tyrosine kinase whose ligands are specific members of the fibroblast growth factor family. The FGFR1 gene is localized to 8p12-p11.2 by in situ hybridization. FGFR1 is essential for the normal formation of the organ of Corti and that phenotype severity observed in FGFR1 mutants is dependent on the dose of FGFR1.Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer and autosomal dominant Kallmann syndrome 2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1477 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Liver Tissue, Rat Brain Tissue, SMMC Cell, HT1080 Cell, MM231 Cell,
IHC: Human Lung Cancer Tissue
Validation Images & Assay Conditions
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Anti-FGFR1 antibody, PA1477, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: SMMC Cell Lysate
Lane 4: HT1080 Cell Lysate
Lane 5: MM231 Cell Lysate
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Anti-FGFR1 antibody, PA1477, IHC(P)
IHC(P): Human Lung Cancer Tissue
Specific Publications For Anti-FGFR1 Antibody Picoband® (PA1477)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-FGFR1 Antibody Picoband®
Question
Can PA1477 work well on mouse brain sample?
Verified customer
Asked: 2019-12-11
Answer
The Anti-FGFR1 Antibody PA1477 works well on mouse brain sample. here is a link to access our IHC protocols, https://www.bosterbio.com/protocol-and-troubleshooting/ihc-protocol
Boster Scientific Support
Answered: 2019-12-11
Question
My lab would like using your anti-FGFR1 antibody for positive regulation of mitotic cell cycle dna replication studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.
T. Dhar
Verified customer
Asked: 2019-04-16
Answer
We appreciate your inquiry. This PA1477 anti-FGFR1 antibody is tested on rat liver tissue, tissue lysate, brain tissue, smmc cell lysate, ht1080 cell lysate, mm231 cell lysate, lung cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-04-16
Question
We are currently using anti-FGFR1 antibody PA1477 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-20
Answer
The anti-FGFR1 antibody (PA1477) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-20
Question
We have been able to see staining in mouse liver. Any tips? Is anti-FGFR1 antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2018-10-16
Answer
Based on literature liver does express FGFR1. Based on Uniprot.org, FGFR1 is expressed in body of pancreas, placenta, neonatal brain stem, teratocarcinoma, lung, liver, placenta testis, brain colon, pancreas, testis uterus, foreskin fibroblast umbilical vein, plasma, among other tissues. Regarding which tissues have FGFR1 expression, here are a few articles citing expression in various tissues:
Brain, and Colon, Pubmed ID: 16421571
Foreskin fibroblast, and Umbilical vein, Pubmed ID: 1652059
Liver, Pubmed ID: 1846977
Lung, Pubmed ID: 1650441
Neonatal brain stem, Pubmed ID: 1697263
Pancreas, Testis, and Uterus, Pubmed ID: 15489334
Placenta, Pubmed ID: 2159626, 2162671
Placenta, and Testis, Pubmed ID: 14702039
Plasma, Pubmed ID: 16335952
Teratocarcinoma, Pubmed ID: 1722683
Boster Scientific Support
Answered: 2018-10-16
Question
Our lab were satisfied with the WB result of your anti-FGFR1 antibody. However we have seen positive staining in placenta testis cell membrane using this antibody. Is that expected? Could you tell me where is FGFR1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-07-13
Answer
According to literature, placenta testis does express FGFR1. Generally FGFR1 expresses in cell membrane. Regarding which tissues have FGFR1 expression, here are a few articles citing expression in various tissues:
Brain, and Colon, Pubmed ID: 16421571
Foreskin fibroblast, and Umbilical vein, Pubmed ID: 1652059
Liver, Pubmed ID: 1846977
Lung, Pubmed ID: 1650441
Neonatal brain stem, Pubmed ID: 1697263
Pancreas, Testis, and Uterus, Pubmed ID: 15489334
Placenta, Pubmed ID: 2159626, 2162671
Placenta, and Testis, Pubmed ID: 14702039
Plasma, Pubmed ID: 16335952
Teratocarcinoma, Pubmed ID: 1722683
Boster Scientific Support
Answered: 2017-07-13
Question
We purchased anti-FGFR1 antibody for IHC on body of pancreas a few years ago. I am using human, and We are going to use the antibody for WB next. I am looking for examining body of pancreas as well as placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
N. Brown
Verified customer
Asked: 2013-02-19
Answer
I have checked the website and datasheets of our anti-FGFR1 antibody and it seems that PA1477 has been tested on human in both IHC and WB. Thus PA1477 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-02-19
