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- Table of Contents
FGFR1 antibody (PA1477) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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Anti-FGFR1 antibody, PA1477, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
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Anti-FGFR1 Antibody Picoband®
FGFR1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
FGFR1 antibody was positively detected by WB in:
Rat Liver Tissue, Rat Brain Tissue, SMMC Cell, HT1080 Cell, MM231 Cell,
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FGFR1 antibody was positively detected by IHC in:
Human Lung Cancer Tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Rat, Mouse
Western blot
0.1-0.5μg/ml
Human, Rat, Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Rat
Tested
Human
Tested
Recommend
Bovine
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-FGFR1 Antibody Picoband®
View all FGFR1 Antibodies
View all FGFR1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human FGFR1, different from the related mouse sequence by two amino acids and rat sequence by three amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
92 kDa
Calculated Molecular Weight
91.9 kDa
Background of FGFR1
FGFR1, Fibroblast growth factor receptor 1, also known as basic fibroblast growth factor receptor 1, fms-related tyrosine kinase-2/Pfeiffer syndrome, and CD331, is a receptor tyrosine kinase whose ligands are specific members of the fibroblast growth factor family. The FGFR1 gene is localized to 8p12-p11.2 by in situ hybridization. FGFR1 is essential for the normal formation of the organ of Corti and that phenotype severity observed in FGFR1 mutants is dependent on the dose of FGFR1.Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer and autosomal dominant Kallmann syndrome 2.
Cite This Product
Anti-FGFR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1477)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
94%
High Homology
Cow
94%
High Homology
Dog
94%
High Homology
Monkey
94%
High Homology
Mouse
88%
Moderate Homology
Rat
88%
Moderate Homology
Sheep
88%
Moderate Homology
Rabbit
82%
Moderate Homology
Zebrafish
35%
Low homology
Drosophila
12%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for FGFR1 antibody
Immunohistochemistry (IHC) protocol for FGFR1 antibody
Publications
Application:
Species:
Zhang JJ,Zhou XH,Zhou Y,Wang YG,Qian BZ,He AN,Shen Z,Hu HY,Yao Y.Bufalin suppresses the migration and invasion of prostate cancer cells through HOTAIR, the sponge of miR-520b.Acta Pharmacol Sin.2019 Sep;40(9):1228-1236.doi:10.1038/s41401-019-0234-8.Epub 2019 Apr 26.PMID:31028291;PMCID:PMC6786369.
Species: Human,Rat
PMID: 31028291
Chen Y,Zhang S,Bai C,Guan Z,Chen W.Circ_0000885 Enhances Osteosarcoma Progression by Increasing FGFR1 Expression via Sponging MiR-1294.Cancer Manag Res.2020 Jul 28;12:6441-6452.doi:10.2147/CMAR. S244382.PMID:32801884;PMCID:PMC7395693.
Species: Human, Mouse
PMID: 32801884
Regulation of 25-hydroxyvitamin D-1-hydroxylase and 24-hydroxylase in keratinocytes by PTH and FGF23
PMID: 30066343
Zhang H, Zhang S, Zhuang H, Lu F. Cancer Biother Radiopharm. 2006 Aug;21(4):321-32. Cytotoxicity Of A Novel Fibroblast Growth Factor Receptor Targeted Immunotoxin On A Human Ovarian Teratocarcinoma Cell Line.
PMID: 16999598
Lin J, Jia Y, Zeng W, Mi Y, Zhang C. Reprod Domest Anim. 2012 Feb;47(1):135-42. Doi: 10.1111/J.1439-0531.2011.01813.X. Epub 2011 May 26. Basic Fgf Promotes Proliferation Of Ovarian Granulosa Cells In The Laying Chickens Via Fgfr1 And Pkc Pathway.
PMID: 21615804
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Product Promise
We promise Anti-FGFR1 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
PA1477
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026